LOCUS       62056_22835        8726 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2160..2177
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2394..2480
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2572..2599
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2618..2709
                     /label=AmpR promoter
     rep_origin      complement(3096..3684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6538..7194)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(8346..8423)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        8653..8681
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    8689..8705
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattaagctt aaaggaggac aaccatgcca ctgttcggat ccttctccga ccgcgtcaac
       61 cgtgcagtgc tctacaggat ctgtgcaaag gaggacaact aatgtttaac aaccgtatcc
      121 gcactgcagc tcttgctggt gcaatcgcaa tctccaccgc agcttccggc gttgctatcc
      181 cagcattcgc tctcccggcg aaccaccgat cggggtcgat cgaggacgtc gagcacatcg
      241 tcgtcctgat gcaggagaac cgttccttcg accactactt cggcacactg cgcggggtcc
      301 gcggcttcgg cgacccgcat ccggtgcgac tggacgacgg caggtcggtg tggcaccagc
      361 gcaagggcga cggcaccgag gtactaccct tccacccgga ggccgacgac ctcggtatgc
      421 agttcctcga agggctcccg cacggctggt ccgacggcca ggacgcctac cacgacgcca
      481 agtacgaccg ctggctgccc gccaagggca ccaccaccat ggcgtacctg acccgcgagg
      541 acatcccctt ccactacgcg ctcgccgaca ccttcaccgt ctgcgacgcc taccactgct
      601 ccttcatcgg ctccaccgac cccaaccgct actacctgtg gtcggggcac acgggcaacg
      661 acggcgcggg cggcggcccg gtcctcggca acgacgaact cggctacgac tggacgacgt
      721 accccgagcg cctggaggcg gccggggtct cctggaagat ctaccaggac gtcggcgacg
      781 gcctcgacgc ggccggccac tggggctgga tcgacgacgc ctaccggggc aactacggcg
      841 acaactccct gctgtacttc aacaagtacc ggaacgcgaa gcccggcgac ccgctgtacg
      901 acaaggcccg caccggcacc gacgcgaagc gcggcgaggg ctacttcgac cggctccgtg
      961 ccgacgtcaa ggcgggcaag ctgccggaga tctcctggat agccgccccg gaggcattct
     1021 ccgagcactc caactggccg tcgaactacg gcgcctggta catctcccag gtcctggacg
     1081 cgctcacctc caacccgaag gtgtgggcga agaccgccct gttcatcacg tacgacgaga
     1141 acgacggctt cttcgaccac gtggtgccgc cgctgccgcc gaagtccgcc gcgcagggcc
     1201 ggtccaccgt cgacgtctcc ctggacgtct tcgagggcag cgccaagcac cgcgagggct
     1261 tctacgggct cggcccgcgc gtgcccatgc tggtcgtctc gccgtggagc aagggcggct
     1321 tcgtctgctc cgagaccttc gaccacacct cggtcatccg cttcatggaa cgccgcttcg
     1381 gcgtccacga gccgaacatc tcaccctggc ggcgcgccgt ctgcggcgac ctgacctccg
     1441 ccttcgactt ctcccgcacg gacagccgcc cggcccggct gccggacacc gacgcctacg
     1501 agccgccgga ccgcgaacgc caccccgact accgtccgac gccgcccgcg gaccccggca
     1561 tgccccggca ggaacgcggg acacgcccca cccggccgct gcgctacgcc ccgcacgtcg
     1621 acggcgccgt ggacacggcg gccgggaagt tcacgctggc cttcgcctcc gggccccggg
     1681 ccggtgccgc cttcctcgtc acctccggca accgcaccga cggcccctgg acgtacacca
     1741 ccgaggccgg caaggacatc gcggacacct ggaactcggc ctactccggc ggctcctacg
     1801 acctgaccgt gcacggcccg gccggcttcc tgcgggtctt ccggggtgcc aacgaggtcg
     1861 ccggacccgc cgggcccgag gtcaccgcac ggcaccgcgg cgacgacctc cggctgaccc
     1921 tcacccacac cgggaccggc accgtccggc tccggatcgc gaacgcccac gacggccgca
     1981 cccggtccgt caccgtccgc cccggcgcca ccgtgcacca caccgtcggc ctcgcgcgca
     2041 gccggcgctg gtacgacgtg acggtcacct ccgagaccgc cccggccttc ctgcgccgct
     2101 tcgccgggca cgtggagaac ggccggccgg ggaccagcga cccggcgatc gtcacggacc
     2161 atcatcacca tcaccattga gaattcagct tggctgtttt ggcggatgag agaagatttt
     2221 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     2281 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     2341 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     2401 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2461 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     2521 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
     2581 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
     2641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2881 tttcgccccg aagaacgttt tccaatgatg agcacttttg cttcctcgct cactgactcg
     2941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     3301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3781 acctagatcc ttttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     3841 atccagccat tcggggtcgt tcactggttc ccctttctga tttctggcat agaagaaccc
     3901 ccgtgaactg tgtggttccg ggggttgctg atttttgcga gacttctcgc gcaattccct
     3961 agcttaggtg aaaacaccat gaaacactag ggaaacaccc atgaaacacc cattagggca
     4021 gtagggcggc ttcttcgtct agggcttgca tttgggcggt gatctggtct ttagcgtgtg
     4081 aaagtgtgtc gtaggtggcg tgctcaatgc actcgaacgt cacgtcattt accgggtcac
     4141 ggtgggcaaa gagaactagt gggttagaca ttgttttcct cgttgtcggt ggtggtgagc
     4201 ttttctagcc gctcggtaaa cgcggcgatc atgaactctt ggaggttttc accgttctgc
     4261 atgcctgcgc gcttcatgtc ctcacgtagt gccaaaggaa cgcgtgcggt gaccacgacg
     4321 ggcttagcct ttgcctgcgc ttctagtgct tcgatggtgg cttgtgcctg cgcttgctgc
     4381 gcctgtagtg cctgttgagc ttcttgtagt tgctgttcta gctgtgcctt ggttgccatg
     4441 ctttaagact ctagtagctt tcctgcgata tgtcatgcgc atgcgtagca aacattgtcc
     4501 tgcaactcat tcattatgtg cagtgctcct gttactagtc gtacatactc atatttacct
     4561 agtctgcatg cagtgcatgc acatgcagtc atgtcgtgct aatgtgtaaa acatgtacat
     4621 gcagattgct gggggtgcag ggggcggagc caccctgtcc atgcggggtg tggggcttgc
     4681 cccgccggta cagacagtga gcaccggggc acctagtcgc ggataccccc cctaggtatc
     4741 ggacacgtaa ccctcccatg tcgatgcaaa tctttaacat tgagtacggg taagctggca
     4801 cgcatagcca agctaggcgg ccaccaaaca ccactaaaaa ttaatagtcc ctagacaaga
     4861 caaacccccg tgcgagctac caactcatat gcacgggggc cacataaccc gaaggggttt
     4921 caattgacaa ccatagcact agctaagaca acgggcacaa cacccgcaca aactcgcact
     4981 gcgcaacccc gcacaacatc gggtctaggt aacactgagt aacactgaaa tagaagtgaa
     5041 cacctctaag gaaccgcagg tcaatgaggg ttctaaggtc actcgcgcta gggcgtggcg
     5101 taggcaaaac gtcatgtaca agatcaccaa tagtaaggct ctggcggggt gccataggtg
     5161 gcgcagggac gaagctgttg cggtgtcctg gtcgtctaac ggtgcttcgc agtttgaggg
     5221 tctgcaaaac tctcactctc gctgggggtc acctctggct gaattggaag tcatgggcga
     5281 acgccgcatt gagctggcta ttgctactaa gaatcacttg gcggcgggtg gcgcgctcat
     5341 gatgtttgtg ggcactgttc gacacaaccg ctcacagtca tttgcgcagg ttgaagcggg
     5401 tattaagact gcgtactctt cgatggtgaa aacatctcag tggaagaaag aacgtgcacg
     5461 gtacggggtg gagcacacct atagtgacta tgaggtcaca gactcttggg cgaacggttg
     5521 gcacttgcac cgcaacatgc tgttgttctt ggatcgtcca ctgtctgacg atgaactcaa
     5581 ggcgtttgag gattccatgt tttcccgctg gtctgctggt gtggttaagg ccggtatgga
     5641 cgcgccactg cgtgagcacg gggtcaaact tgatcaggtg tctacctggg gtggagacgc
     5701 tgcgaaaatg gcaacctacc tcgctaaggg catgtctcag gaactgactg gctccgctac
     5761 taaaaccgcg tctaaggggt cgtacacgcc gtttcagatg ttggatatgt tggccgatca
     5821 aagcgacgcc ggcgaggata tggacgctgt tttggtggct cggtggcgtg agtatgaggt
     5881 tggttctaaa aacctgcgtt cgtcctggtc acgtggggct aagcgtgctt tgggcattga
     5941 ttacatagac gctgatgtac gtcgtgaaat ggaagaagaa ctgtacaagc tcgccggtct
     6001 ggaagcaccg gaacgggtcg aatcaacccg cgttgctgtt gctttggtga agcccgatga
     6061 ttggaaactg attcagtctg atttcgcggt taggcagtac gttctcgatt gcgtggataa
     6121 ggctaaggac gtggccgctg cgcaacgtgt cgctaatgag gtgctggcaa gtctgggtgt
     6181 ggattccacc ccgtgcatga tcgttatgga tgatgtggac ttggacgcgg ttctgcctac
     6241 tcatggggac gctactaagc gtgatctgaa tgcggcggtg ttcgcgggta atgagcagac
     6301 tattcttcgc acccactaaa agcggcataa accccgttcg atattttgtg cgatgaattt
     6361 atggtcaatg tcgcgggggc aaactatgat gggtcttgtt gttggcgtcc cggaaaacga
     6421 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
     6481 tgggcgtcgc ttggtcggtc atttcgaagg gcaccaataa ctgccttaaa aaaattacgc
     6541 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     6601 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     6661 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     6721 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     6781 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     6841 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     6901 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     6961 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     7021 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     7081 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     7141 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     7201 ttccttagct cctgaaaatc tcgtcgaagc tcggcggatt tgtcctactc aagctgatcc
     7261 gacaaaatcc acacattatc ccaggtgtcc ggatcggtca aatacgctgc cagctcatag
     7321 accgtatcca aagcatccgg ggctgatccc cggcgccagg gtggtttttc ttttcaccag
     7381 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
     7441 gtccacgtgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     7501 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
     7561 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     7621 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     7681 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     7741 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     7801 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
     7861 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     7921 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     7981 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     8041 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     8101 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     8161 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     8221 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     8281 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     8341 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     8401 gttttgcacc attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca
     8461 agctgatccg ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     8521 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     8581 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     8641 tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
     8701 aacaatttca cacaggaaac agaatt
//
