LOCUS       .                       6284 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             309..1310
                     /label="repB"
                     /note="RepB replication protein"
     promoter        2309..2400
                     /label="AmpR promoter"
     CDS             2401..3258
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3336..3881
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             6208..6225
                     /label="6xHis"
                     /note="6xHis affinity tag"
ORIGIN
        1 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
       61 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
      121 atggttttga acttgttctt tcttatcttg atacatatag aaataacgtc atttttattt
      181 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
      241 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgact aaacaaataa
      301 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
      361 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
      421 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
      481 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
      541 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
      601 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
      661 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
      721 taaataagag tttgtcagat atggctcaag gatttcgccg aatgatgcaa tataaaaaaa
      781 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
      841 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
      901 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
      961 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
     1021 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     1081 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     1141 ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca
     1201 cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt
     1261 ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca
     1321 aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc
     1381 ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta
     1441 gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa
     1501 tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaat
     1561 atggggatga tgttaaggct attggtgttt atggctctct tggtcgtcag actgatgggc
     1621 cctattcgga tattgagatg atgtgtgtca tgtcaacaga ggaagcagag ttcagccatg
     1681 aatggacaac cggtgagtgg aaggtggaag tgaattttga tagcgaagag attctactag
     1741 attatgcatc tcaggtggaa tcagattggc cgcttacaca tggtcaattt ttctctattt
     1801 tgccgattta tgattcaggt ggatacttag agaaagtgta tcaaactgct aaatcggtag
     1861 aagcccaaac gttccacgat gcgatttgtg cccttatcgt agaagagctg tttgaatatg
     1921 caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac atttctacca tccttgactg
     1981 tacaggtagc aatggcaggt gccatgttga ttggtctgca tcatcgcatc tgttatacga
     2041 cgagcgcttc ggtcttaact gaagcagtta agcaatcaga tcttccttca ggttatgacc
     2101 atctgtgcca gttcgtaatg tctggtcaac tttccgactc tgagaaactt ctggaatcgc
     2161 tagagaattt ctggaatggg attcaggagt ggacagaacg acacggatat atagtggatg
     2221 tgtcaaaacg cataccattt tgaacgatga cctctaataa ttgttaatca tgttggttac
     2281 gtatttatta acttctccta gtattagttt tgtttatttt tctaaataca ttcaaatatg
     2341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2641 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2941 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3121 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3241 tcactgatta agcattggta atgcgtttct acaaactctt gggcctagtg ttttagatcc
     3301 tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta
     3361 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
     3421 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
     3481 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
     3541 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
     3601 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     3661 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     3721 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     3841 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaattatcat ggctgtcatg
     3901 gcgcattaac ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat aagaaaaagg
     3961 attaattatg agcgaattga attaataata aggtaataga tttacattag aaaatgaaag
     4021 gggattttat gcgtgagaat gttacagtct atcccggcat tgccagtcgg ggatattaaa
     4081 aagagtatag gtttttattg cgataaacta ggtttcactt tggttcacca tgaagatgga
     4141 ttcgcagttc taatgtgtaa tgaggttcgg attcatctat gggaggcaag tgatgaaggc
     4201 tggcgctctc gtagtaatga ttcaccggtt tgtacaggtg cggagtcgtt tattgctggt
     4261 actgctagtt gccgcattga agtagaggga attgatgaat tatatcaaca tattaagcct
     4321 ttgggcattt tgcaccccaa tacatcatta aaagatcagt ggtgggatga acgagacttt
     4381 gcagtaattg atcccgacaa caatttgatt agcttttttc aacaaataaa aagctaaaat
     4441 ctattattaa tctgttcagc aatcgggcgc gattgctgaa taaaagatac gagagacctc
     4501 tcttgtatct tttttatttt gagtggtttt gtccgttaca ctagaaaacc gaaagacaat
     4561 aaaaatttta ttcttgctga gtctggcttt cggtaagcta gacaaaacgg acaaaataaa
     4621 aattggcaag ggtttaaagg tggagatttt ttgagtgatc ttctcaaaaa atactacctg
     4681 tcccttgctg atttttaaac gagcacgaga gcaaaacccc cctttgctga ggtggcagag
     4741 ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg
     4801 ttttggtcgg cactgaattc gagctcagca ttattgagtg gatgattata ttccttttga
     4861 taggtggtat gttttcgctt gaacttttaa atacagccat tgaacatacg gttgatttaa
     4921 taactgacaa acatcaccct cttgctaaag cggccaagga cgctgccgcc ggggctgttt
     4981 gcgtttttgc cgtgatttcg tgtatcattg gtttacttat ttttttgcca aagctgtaat
     5041 ggctgaaaat tcttacattt attttacatt tttagaaatg ggcgtgaaaa aaagcgcgcg
     5101 attatgtaaa atataaagtg atagcggtac caggagggct ggaagaagca gaccgctaac
     5161 acagtacata aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac
     5221 agtattaacc tttactaccg cactgctggc aggaggcgca actcaagctt aatgaaaaaa
     5281 catgctattg cagtaatgat gatcgccgta ttttctgagt cggtttatgc ggagtctgcc
     5341 ttatttattc cggacgtctc tcctgatagc gtcacgacat ccctttccgt gggtgtgtta
     5401 aatggtaaat ccagggagct ggtttatgat accgacaccg ggcggaagct gagtcaactg
     5461 gattggaaaa taaaaaatgt cgccacgttg cagggggatt tatcatggga accctattcg
     5521 ttcatgacgc tggacgcccg cggctggacg tctttggcgt cgggatcggg tcatatggtt
     5581 gaccatgact ggatgagcag tgagcagcca ggctggaccg atcgttcaat tcatccggac
     5641 accagcgtca actatgctaa tgaatacgat ttgaacgtga aaggttggtt attgcagggc
     5701 gataactaca aggcgggcgt aacagcgggc tatcaggaaa cccgttttag ctggacggca
     5761 agaggcgggt cttatattta tgataatggt cgatatattg gtaattttcc tcatggcgtg
     5821 cgcggcatag gttatagcca gcgtttcgaa atgccctata tcgggctggc gggtgattat
     5881 cgtattaatg actttgagtg taatgtactg tttaaataca gcgactgggt aaatgcgcat
     5941 gataatgacg aacactacat gcgcaaactt accttccgtg aaaaaacgga aaattcacga
     6001 tattatggcg cttctattga cgccggatat tatattacca gtaatgcaaa aatctttgct
     6061 gagtttgctt acagtaaata tgaagaaggt aagggcggta cgcaaatcat agataaaacc
     6121 agcggtgata cggcgtattt tggtggcgat gccgcaggta tagctaataa taactatacg
     6181 gttaccgcgg ggttgcagta tcgcttccac caccaccacc accactaggt cgacctgcag
     6241 gcatgcaagc tagcttcagc acaattccaa gaaagacacg attt
//
