LOCUS       V012891                 8328 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012891
VERSION     V012891
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2819..2835)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3309..3413
                     /label="AmpR promoter"
     CDS             3414..4271
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4445..5033
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5321..5342
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5357..5387
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5395..5411
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5419..5435
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5456..5474
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5879..6319
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     terminator      6358..6444
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6463..6490
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             7104..7838
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga accagaaaca
       61 gaatcagaac aagtaacatt ggatttagaa atggaagaag aaaaaagcaa tgacttcgtg
      121 tgaataatgc acgaaatcgt tgcttatttt tttttaaaag cggtatacta gatataacga
      181 aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac atttataaaa tgtttgacga
      241 cattttataa atgcatagcc cgataagatt gccaaaccaa cgcttatcag ttagtcagat
      301 gaactcttcc ctcgtaagaa gttatttaat taactttgtt tgaagacggt atataaccgt
      361 actatcatta tatagggaaa tcagagagtt ttcaagtatc taagctactg aatttaagaa
      421 ttgttaagca atcaatcgga aatcgtttga ttgctttttt tgtattcatt tatagaaggt
      481 ggagtttgta tgaatcatga tgaatgtaaa acttatataa aaaatagttt attggagata
      541 agaaaattag caaatatcta tacactagaa acgtttaaga aagagttaga aaagagaaat
      601 atctacttag aaacaaaatc agataagtat ttttcttcgg agggggaaga ttatatatat
      661 aagttaatag aaaataacaa aataatttat tcgattagtg gaaaaaaatt gacttataaa
      721 ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa
      781 gttaattaaa caacctattt tataggattt ataggaaagg agaacagctg aatgaatatc
      841 ccttttgttg tagaaactgt gcttcatgac ggcttgttaa agtacaaatt taaaaatagt
      901 aaaattcgct caatcactac caagccaggt aaaagcaaag gggctatttt tgcgtatcgc
      961 tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga cttccgagga agcgattcaa
     1021 gaaaatcaag atacatttac acattggaca cccaacgttt atcgttatgg aacgtatgca
     1081 gacgaaaacc gttcatacac gaaaggacat tctgaaaaca atttaagaca aatcaatacc
     1141 ttctttattg attttgatat tcacacggca aaagaaacta tttcagcaag cgatatttta
     1201 acaaccgcta ttgatttagg ttttatgcct actatgatta tcaaatctga taaaggttat
     1261 caagcatatt ttgttttaga aacgccagtc tatgtgactt caaaatcaga atttaaatct
     1321 gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat attttggaaa gtctttgcca
     1381 gttgatctaa cgtgtaatca ttttggtatt gctcgcatac caagaacgga caatgtagaa
     1441 ttttttgatc ctaattaccg ttattctttc aaagaatggc aagattggtc tttcaaacaa
     1501 acagataata agggctttac tcgttcaagt ctaacggttt taagcggtac agaaggcaaa
     1561 aaacaagtag atgaaccctg gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa
     1621 aagggtttaa tagggcgtaa taacgtcatg tttaccctct ctttagccta ctttagttca
     1681 ggctattcaa tcgaaacgtg cgaatataat atgtttgagt ttaataatcg attagatcaa
     1741 cccttagaag aaaaagaagt aatcaaaatt gttagaagtg cctattcaga aaactatcaa
     1801 ggggctaata gggaatacat taccattctt tgcaaagctt gggtatcaag tgatttaacc
     1861 agtaaagatt tatttgtccg tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt
     1921 caacgtgttc atttgtcaga atggaaagaa gatttaatgg cttatattag cgaaaaaagc
     1981 gatgtataca agccttattt agtgacgacc aaaaaagaga ttagagaagt gctaggcatt
     2041 cctgaacgga cattagataa attgctgaag gtactgaagg cgaatcagga aattttcttt
     2101 aagattaaac caggaagaaa tggtggcatt caacttgcta gtgttaaatc attgttgcta
     2161 tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct gacaaattct
     2221 tttgacttag agcatacatt cattcaagag actttaaaca agctagcaga acgccctaaa
     2281 acggacacac aactcgattt gtttagctat gatacaggct gaaaataaaa cccgcactat
     2341 gccattacat ttatatctat gatacgtgtt tgttttttct ttgctgttta gcgaatgatt
     2401 agcagaaata tacagagtaa gattttaatt aattattagg gggagaagga gagagtagcc
     2461 cgaaaacttt tagttggctt ggactgaacg aagtgaggga aaggctacta aaacgtcgag
     2521 gggcagtgag agcgaagcga acacttgatt ttttaatttt ctatctttta taggtcatta
     2581 gagtatactt atttgtccta taaactattt agcagcataa tagatttatt gaataggtca
     2641 tttaagttga gcatattaga ggaggaaaat cttggagaaa tatttgaaga acccgattac
     2701 atggattgga ttagttcttg tggttacgtg gtttttaact aaaagtagtg aatttttgat
     2761 ttttggtgtg tgtgtcttgt tgttagtatt tgctagtcaa agtgattaaa tagaattcac
     2821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2941 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     3001 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3061 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3181 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4561 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     5461 accctcacta aagggaacaa aagctgggta ccgggccccc cctcgacggt atcgatagcc
     5521 cgcctaatga gcgggctttt ttttgatccc ccaaagaagc gcgtaatatc aggtttaaga
     5581 gacaataaga aaaaaagatt gaaaaaagtg acattaaatt cttgacaggg agagataggt
     5641 ttgatagaat ataataggtt tgatagaata taatagggta ccgagctcga attcctgcag
     5701 cccaaatcgc gaaaccgaac ttaatgggag gaaaaattaa aaaagaacag ttatgaaaaa
     5761 aaagattatc tcagctattt taatgtctac agtgatactt tctgctgcag ccccgttgtc
     5821 aggtgtttac gctgacacaa actcagatat tgctaaacaa gatgcggtcg acccattgtc
     5881 aactaaaaaa ttacataaag aacctgcgac tttaattaaa gcgattgatg gtgatacggt
     5941 taaattaatg tacaaaggtc aaccaatgac attcagacta ttattggttg atacacctga
     6001 aacaaagcat cctaaaaaag gtgtagagaa atatggtcct gaagcaagtg catttacgaa
     6061 aaaaatggta gaaaatgcaa agaaaattga agtcgagttt gacaaaggtc aaagaactga
     6121 taaatatgga cgtggcttag cgtatattta tgctgatgga aaaatggtaa acgaagcttt
     6181 agttcgtcaa ggcttggcta aagttgctta tgtttacaaa cctaacaata cacatgaaca
     6241 acatttaaga aaaagtgaag cacaagcgaa aaaagagaaa ttaaatattt ggagcgaaga
     6301 caacgctgat tcaggtcaaa tcgatctcga gatatcgcta gttctagatt gaggcatcaa
     6361 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     6421 aacgctctcc tgagtaggac aaatccgccg ctctagctaa gcagaaggcc atcctgacgg
     6481 atggcctttt tgcgtttcta caaactcttg ttggggatcc gcctgcagcc aagcttatcg
     6541 attctagact cgagagctct cgagtctaga atcgatacga ttttgaagtg gcaacagata
     6601 aaaaaaagca gtttaaaatt gttgctgaac ttttaaaaca agcaaataca atcattgtcg
     6661 caacagatag cgacagagaa ggcgaaaaca ttgcctggtc gatcattcat aaagcaaatg
     6721 ccttttctaa agataaaacg tataaaagac tatggatcaa tagtttagaa aaagatgtga
     6781 tccgtagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt tatcaagaag
     6841 cgcaaaagaa aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa
     6901 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg
     6961 cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat
     7021 agtgcagtat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
     7081 tgtaattaag aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa
     7141 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg
     7201 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac
     7261 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac
     7321 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac
     7381 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag
     7441 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc
     7501 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc
     7561 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa
     7621 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact
     7681 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc
     7741 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt
     7801 ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt
     7861 aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg tatactactg
     7921 acagcttcca aggagctaaa gaggtcccta gcgcttagaa tcgctttagg aaacacgatc
     7981 cagtccaata atcgtcgata aaaacttttg aaaaaggttg gtgaaattac ctacttttgg
     8041 aataatcaca aatcacaagt gattaatcac aaatcacaag tgattaatca cttgtttatt
     8101 aagatattaa aagctataat ttaaataaag cgtgaatttt attacacaaa aagagggggg
     8161 agaaacttgg aactagcatt tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa
     8221 gaaaaattct cccaagaatt agaaatgagt agatcaaatt attcacgaat agaatcagga
     8281 aaatcagatc caaccataaa aacactagaa caaattgcaa agttaact
//
