LOCUS       40924_45758        9287 bp DNA     circular SYN 18-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(117..705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1249..2061)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     enhancer        2686..3065
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3066..3269
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3405..3674
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     CDS             3728..4144
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    8504..8878
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     polyA_signal    9085..9140
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
       61 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcgg gggggggggc
      961 gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat
     1021 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt
     1081 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
     1141 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
     1201 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
     1261 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     1321 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     1381 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     1441 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     1501 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     1561 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     1621 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     1681 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     1741 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     1801 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     1861 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     1921 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     1981 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     2041 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     2101 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     2161 aacgtggctt tccccccccc ccattattga agcatttatc agggttattg tctcatgagc
     2221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2281 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2341 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2401 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2461 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2521 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     2581 aggagaaaat accgcatcag attggctatt ggccattgca tacgttgtat ccatatcata
     2641 atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga
     2701 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2761 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2821 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2881 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2941 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3001 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3061 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3121 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3181 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3241 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     3301 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
     3361 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
     3421 gactctatag gcacacccct ttggctctta tgcatgctat actgtttttg gcttggggcc
     3481 tatacacccc cgcttcctta tgctataggt gatggtatag cttagcctat aggtgtgggt
     3541 tattgaccat tattgaccac tccaacggtg gagggcagtg tagtctgagc agtactcgtt
     3601 gctgccgcgc gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg
     3661 ggtcttttct gcagtcaccg tcgtcgacgg tatcgataag cttgatatcg aattcgccgc
     3721 caccatgggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
     3781 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     3841 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     3901 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     3961 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     4021 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     4081 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     4141 cgccaaacct aaacctcaag ttctttctga cagtgggggg ccgctcatcg acctacttac
     4201 agaagacccc ccgccttata gggacccaag accaccccct tccgacaggg acggaaatgg
     4261 tggagaagcg acccctgcgg gagaggcacc ggacccctcc ccaatggcat ctcgcctacg
     4321 tgggagacgg gagccccctg tggccgactc cactacctcg caggcattcc ccctccgcgc
     4381 aggaggaaac ggacagcttc aatactggcc gttctcctct tctgaccttt acaactggaa
     4441 aaataataac ccttcttttt ctgaagatcc aggtaaactg acagctctga tcgagtctgt
     4501 cctcatcacc catcagccca cctgggacga ctgtcagcag ctgttgggga ctctgctgac
     4561 cggagaagaa aaacaacggg tgctcttaga ggctagaaag gcggtgcggg gcgatgatgg
     4621 gcgccccact caactgccca atgaagtcga tgccgctttt cccctcgagc gcccagactg
     4681 ggattacacc acccaggcag gtaggaacca cctagtccac tatcgccagt tgctcctagc
     4741 gggtctccaa aacgcgggca gaagccccac caatttggcc aaggtaaaag gaataacaca
     4801 agggcccaat gagtctccct cggccttcct agagagactt aaggaagcct atcgcaggta
     4861 cactccttat gaccctgagg acccagggca agaaactaat gtgtctatgt ctttcatttg
     4921 gcagtctgcc ccagacattg ggagaaagtt agagaggtta gaagatttaa aaaacaagac
     4981 gcttggagat ttggttagag aggcagaaaa gatctttaat aaacgagaaa ccccggaaga
     5041 aagagaggaa cgtatcagga gagaaacaga ggaaaaagaa gaacgccgta ggacagagga
     5101 tgagcagaaa gagaaagaaa gagatcgtag gagacataga gagatgagca agctattggc
     5161 cactgtcgtt agtggacaga aacaggatag acagggagga gaacgaagga ggtcccaact
     5221 cgatcgcgac cagtgtgcct actgcaaaga aaaggggcac tgggctaaag attgtcccaa
     5281 gaaaccacga ggacctcggg gaccaagacc ccagacctcc ctcctgaccc tagatgacta
     5341 gggaggtcag ggtcaggagc ccccccctga acccaggata accctcaaag tcggggggca
     5401 acccgtcacc ttcctggtag atactggggc ccaacactcc gtgctgaccc aaaatcctgg
     5461 acccctaagt gataagtctg cctgggtcca aggggctact ggaggaaagc ggtatcgctg
     5521 gaccacggat cgcaaagtac atctagctac cggtaaggtc acccactctt tcctccatgt
     5581 accagactgt ccctatcctc tgttaggaag agatttgctg actaaactaa aagcccaaat
     5641 ccactttgag ggatcaggag ctcaggttat gggaccaatg gggcagcccc tgcaagtgtt
     5701 gaccctaaat atagaagatg agtatcggct acatgagacc tcaaaagagc cagatgtttc
     5761 tctagggtcc acatggctgt ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg
     5821 actggcagtt cgccaagctc ctctgatcat acctctgaaa gcaacctcta cccccgtgtc
     5881 cataaaacaa taccccatgt cacaagaagc cagactgggg atcaagcccc acatacagag
     5941 actgttggac cagggaatac tggtaccctg ccagtccccc tggaacacgc ccctgctacc
     6001 cgttaagaaa ccagggacta atgattatag gcctgtccag gatctgagag aagtcaacaa
     6061 gcgggtggaa gacatccacc ccaccgtgcc caacccttac aacctcttga gcgggctccc
     6121 accgtcccac cagtggtaca ctgtgcttga tttaaaggat gcctttttct gcctgagact
     6181 ccaccccacc agtcagcctc tcttcgcctt tgagtggaga gatccagaga tgggaatctc
     6241 aggacaattg acctggacca gactcccaca gggtttcaaa aacagtccca ccctgtttga
     6301 tgaggcactg cacagagacc tagcagactt ccggatccag cacccagact tgatcctgct
     6361 acagtacgtg gatgacttac tgctggccgc cacttctgag ctagactgcc aacaaggtac
     6421 tcgggccctg ttacaaaccc tagggaacct cgggtatcgg gcctcggcca agaaagccca
     6481 aatttgccag aaacaggtca agtatctggg gtatcttcta aaagagggtc agagatggct
     6541 gactgaggcc agaaaagaga ctgtgatggg gcagcctact ccgaagaccc ctcgacaact
     6601 aagggagttc ctagggacgg caggcttctg tcgcctctgg atccctgggt ttgcagaaat
     6661 ggcagccccc ttgtaccctc tcaccaaaac ggggactctg tttaattggg gcccagacca
     6721 acaaaaggcc tatcaagaaa tcaagcaagc tcttctaact gccccagccc tggggttgcc
     6781 agatttgact aagccctttg aactctttgt cgacgagaag cagggctacg ccaaaggtgt
     6841 cctaacgcaa aaactgggac cttggcgtcg gccggtggcc tacctgtcca aaaagctaga
     6901 cccagtagca gctgggtggc ccccttgcct acggatggta gcagccattg ccgtactgac
     6961 aaaggatgca ggcaagctaa ccatgggaca gccactagtc attctggccc cccatgcagt
     7021 agaggcacta gtcaaacaac cccccgaccg ctggctttcc aacgcccgga tgactcacta
     7081 tcaggccttg cttttggaca cggaccgggt ccagttcgga ccggtggtag ccctgaaccc
     7141 ggctacgctg ctcccactgc ctgaggaagg gctgcaacac aactgccttg atatcctggc
     7201 cgaagcccac ggaacccgac ccgacctaac ggaccagccg ctcccagacg ccgaccacac
     7261 ctggtacacg gatggaagca gtctcttaca agagggacag cgtaaggcgg gagctgcggt
     7321 gaccaccgag accgaggtaa tctgggctaa agccctgcca gccgggacat ccgctcagcg
     7381 ggctgaactg atagcactca cccaggccct aaagatggca gaaggtaaga agctaaatgt
     7441 ttatactgat agccgttatg cttttgctac tgcccatatc catggagaaa tatacagaag
     7501 gcgtgggttg ctcacatcag aaggcaaaga gatcaaaaat aaagacgaga tcttggccct
     7561 actaaaagcc ctctttctgc ccaaaagact tagcataatc cattgtccag gacatcaaaa
     7621 gggacacagc gccgaggcta gaggcaaccg gatggctgac caagcggccc gaaaggcagc
     7681 catcacagag actccagaca cctctaccct cctcatagaa aattcatcac cctacacctc
     7741 agaacatttt cattacacag tgactgatat aaaggaccta accaagttgg gggccattta
     7801 tgataaaaca aagaagtatt gggtctacca aggaaaacct gtgatgcctg accagtttac
     7861 ttttgaatta ttagactttc ttcatcagct gactcacctc agcttctcaa aaatgaaggc
     7921 tctcctagag agaagccaca gtccctacta catgctgaac cgggatcgaa cactcaaaaa
     7981 tatcactgag acctgcaaag cttgtgcaca agtcaacgcc agcaagtctg ccgttaaaca
     8041 gggaactagg gtccgcgggc atcggcccgg cactcattgg gagatcgatt tcaccgagat
     8101 aaagcccgga ttgtatggct ataaatatct tctagttttt atagatacct tttctggctg
     8161 gatagaagcc ttcccaacca agaaagaaac cgccaaggtc gtaaccaaga agctactaga
     8221 ggagatcttc cccaggttcg gcatgcctca ggtattggga actgacaatg ggcctgcctt
     8281 cgtctccaag gtgagtcaga cagtggccga tctgttgggg attgattgga aattacattg
     8341 tgcatacaga ccccaaagct caggccaggt agaaagaatg aatagaacca tcaaggagac
     8401 tttaactaaa ttaacgcttg caactggctc tagagactgg gtgctcctac tccccttagc
     8461 cctgtaccga gcccgcaaca cgccgggccc ccatggcctc accccatatg agatcttata
     8521 tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag ttactaacag
     8581 cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag tctggagacc
     8641 tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc cttaccgagt
     8701 cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc gctggaaagg
     8761 accttaccca gtcctgctga ccacccccac cgccctcaaa gtagacggca tcgcagcttg
     8821 gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct ctagactgac
     8881 atggcgcgtt caacgctctc aaaacccctt aaaaataagg ttaacccgcg aggcccccta
     8941 atccccttaa ttcttctgat gctcagaggg gtcagtgtgg gcccggtacc gtatactcta
     9001 gagcggccgc ggatccagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     9061 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     9121 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     9181 agaatgagta tttggtttag agtttggcaa catatgccca ttcttccgct tcctcgctca
     9241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagct
//
