LOCUS       Exported                4036 bp DNA     circular SYN 03-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      46..397
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             411..1244
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(1241..1495)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLKGRPGRGGHGL"
     primer_bind     1405..1421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1425..1481
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1491..1507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1515..1531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1539..1569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1584..1605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1893..2481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3282..3815)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4004..4032)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cctctcaggc gccgctggtg ccgctggttg gacgccaagg gtgaatccgc ctcgataccc
       61 tgattactcg cttcctgcgc cctctcaggc ggcgataggg gactggtaaa acggggattg
      121 cccagacgcc tcccccgccc cttcaggggc acaaatgcgg ccccaacggg gccacgtagt
      181 ggtgcgtttt ttgcgtttcc acccttttct tccttttccc ttttaaacct tttaggacgt
      241 ctacaggcca cgtaatccgt ggcctgtaga gtttaaaaag ggacggattt gttgccatta
      301 agggacggat ttgttgttaa gaagggacgg atttgttgtt gtaaagggac ggatttgttg
      361 tattgtggga cgcagataca gtgtcccctt atacacaagg aatgtcgaac gtggcctcac
      421 ccccaatggt ttacaaaagc aatgccctgg tcgaggccgc gtatcgcctc agtgttcagg
      481 aacagcggat cgttctggcc tgtattagcc aggtgaagag gagcgagcct gtcaccgatg
      541 aagtgatgta ttcagtgacg gcggaggaca tagcgacgat ggcgggtgtc cctatcgaat
      601 cttcctacaa ccagctcaaa gaagcggccc tgcgcctgaa acggcgggaa gtccggttaa
      661 cccaagagcc caatggcaag gggaaaagac cgagtgtgat gattaccggc tgggtgcaaa
      721 caatcatcta ccgggagggt gagggccgtg tagaactcag gttcaccaaa gacatgctgc
      781 cgtacctgac ggaactcacc aaacagttca ccaaatacgc cttggctgac gtggccaaga
      841 tggacagcac ccacgcgatc aggctttacg agctgctcat gcaatgggac agcatcggcc
      901 agcgcgaaat agaaattgac cagctgcgaa agtggtttca actggaaggc cggtatccct
      961 cgatcaagga cttcaagttg cgagtgcttg atccagccgt gacgcagatc aacgagcaca
     1021 gcccgctaca ggtggagtgg gcgcagcgaa agaccgggcg caaggtcaca catctgttgt
     1081 tcagttttgg accgaagaag cccgccaagg cggtgggtaa ggccccagcg aagcgcaagg
     1141 ccgggaagat ttcagatgct gagatcgcga aacaggctcg ccctggtgag acatgggaag
     1201 cggcccgcgc tcgactaacc cagatgccgc tggatctggc ctagaggccg tggccaccac
     1261 ggcccggcct gcctttcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1321 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1381 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
     1441 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg
     1501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3181 agatgctttt ctgtgactgg tgagtcgaat tggccgcggc gttgtgacaa tttaccgaac
     3241 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     3301 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     3361 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     3421 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3481 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3541 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3601 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3661 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3721 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3781 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3841 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3901 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3961 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     4021 ggcttatgtc aattcg
//
