LOCUS       .                       5265 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2244..2960
                     /label="EYFP"
                     /note="enhanced YFP"
     primer_bind     complement(3000..3016)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3180..4010)
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      4024..4375
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2281 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2341 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2401 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg
     2461 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2521 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2581 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2641 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2701 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2761 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2821 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2881 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2941 gcatggacga gctgtacaag ggatcctcta gagtcgacct gcaggcatgc aagcttggca
     3001 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3061 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3121 ccttcccaac agttgcgcag cctgaaaggc aggccgggcc gtggtggcca cggcctctag
     3181 gccagatcca gcggcatctg ggttagtcga gcgcgggccg cttcccatgt ctcaccaggg
     3241 cgagcctgtt tcgcgatctc agcatctgaa atcttcccgg ccttgcgctt cgctggggcc
     3301 ttacccaccg ccttggcggg cttcttcggt ccaaaactga acaacagatg tgtgaccttg
     3361 cgcccggtct ttcgctgcgc ccactccacc tgtagcgggc tgtgctcgtt gatctgcgtc
     3421 acggctggat caagcactcg caacttgaag tccttgatcg agggataccg gccttccagt
     3481 tgaaaccact ttcgcagctg gtcaatttct atttcgcgct ggccgatgct gtcccattgc
     3541 atgagcagct cgtaaagcct gatcgcgtgg gtgctgtcca tcttggccac gtcagccaag
     3601 gcgtatttgg tgaactgttt ggtgagttcc gtcaggtacg gcagcatgtc tttggtgaac
     3661 ctgagttcta cacggccctc accctcccgg tagatgattg tttgcaccca gccggtaatc
     3721 atcacactcg gtcttttccc cttgccattg ggctcttggg ttaaccggac ttcccgccgt
     3781 ttcaggcgca gggccgcttc tttgagctgg ttgtaggaag attcgatagg gacacccgcc
     3841 atcgtcgcta tgtcctccgc cgtcactgaa tacatcactt catcggtgac aggctcgctc
     3901 ctcttcacct ggctaataca ggccagaacg atccgctgtt cctgaacact gaggcgatac
     3961 gcggcctcga ccagggcatt gcttttgtaa accattgggg gtgaggccac gttcgacatt
     4021 ccttgtgtat aaggggacac tgtatctgcg tcccacaata caacaaatcc gtccctttac
     4081 aacaacaaat ccgtcccttc ttaacaacaa atccgtccct taatggcaac aaatccgtcc
     4141 ctttttaaac tctacaggcc acggattacg tggcctgtag acgtcctaaa aggtttaaaa
     4201 gggaaaagga agaaaagggt ggaaacgcaa aaaacgcacc actacgtggc cccgttgggg
     4261 ccgcatttgt gcccctgaag gggcggggga ggcgtctggg caatccccgt tttaccagtc
     4321 ccctatcgcc gcctgagagg gcgcaggaag cgagtaatca gggtatcgag gcggattcac
     4381 ccttggcgtc caaccagcgg caccagcggc gcctgagagg cctacagagc ggttggacac
     4441 caaggggagg ggctaagacc ggtttatcag tcccctttcc ctcgtttctt tccaacgcga
     4501 tagcccagca aggccgccac cgttgccacc gtcaccccag caagcacagc cagtggcgtg
     4561 taattggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca
     4861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4921 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4981 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgccgc ctgatgcggt
     5041 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     5101 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5221 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
