LOCUS       .                       4273 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      1..642
                     /label="E9 terminator"
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
     primer_bind     complement(659..675)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(683..699)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(707..737)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(752..773)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1061..1649)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1823..2680)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(2681..2785)
                     /label="AmpR promoter"
     primer_bind     3259..3275
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3776..4121
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4166..4192
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4196..4222
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4226..4252
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
ORIGIN
        1 agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat
       61 tgcgcacaca ccagaatcct actgagttcg agtattatgg cattgggaaa catgtttttc
      121 ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac
      181 tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc
      241 aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa aatataagag
      301 atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag
      361 ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa
      421 tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt
      481 tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc
      541 attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta
      601 atgcatttta tgacttgcca attgattgac aacatgcatc aagcttggcg taatcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1501 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2821 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2881 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2941 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3001 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3061 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     3121 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3181 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     3241 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggtccccag attagccttt
     3301 tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca
     3361 tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta
     3421 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
     3481 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc
     3541 aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt
     3601 caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac
     3661 agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
     3721 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
     3781 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
     3841 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
     3901 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     3961 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     4021 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     4081 cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggacg agctcggtac
     4141 cctcgagcca gggccctggt tcgaataccc atacgacgta cctgattatg caacttatcc
     4201 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catgaactag
     4261 tgtcgacctg cag
//
