LOCUS       .                       4252 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      18..659
                     /label="E9 terminator"
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
     primer_bind     complement(676..692)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(700..716)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(724..754)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(769..790)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1078..1666)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1840..2697)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(2698..2802)
                     /label="AmpR promoter"
     primer_bind     3276..3292
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3793..4138
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4183..4248
                     /label="3xFLAG"
                     /note="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
ORIGIN
        1 ctagtgtcga cctgcagagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt
       61 caatgcatca gtttcattgc gcacacacca gaatcctact gagttcgagt attatggcat
      121 tgggaaacat gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
      181 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
      241 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
      301 atttgaaaat ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg
      361 gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt
      421 attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca
      481 agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc
      541 cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag
      601 tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac atgcatcaag
      661 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1501 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2821 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2881 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     2941 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     3001 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     3061 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3121 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3181 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3241 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcgg
     3301 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
     3361 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
     3421 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
     3481 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
     3541 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
     3601 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
     3661 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
     3721 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     3781 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
     3841 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
     3901 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
     3961 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     4021 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     4081 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
     4141 ggggacgagc tcggtaccct cgagccaggg ccctggttcg aagactacaa agaccatgat
     4201 ggagactata aggatcacga catcgattac aaggacgatg acgataagtg aa
//
