LOCUS       .                       8639 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4550..5266
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             6053..6073
                     /label="7xHis"
                     /note="6xHis affinity tag"
     polyA_signal    complement(6299..6420)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7092..7108)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(7145..7163)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7184..7200)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7208..7224)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7232..7262)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7277..7298)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7586..8174)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag accagcaaag ttctagaatt
     1081 tgtcgaaaca tttatgttat atatttcctg aaaaaaattc tgagtaagtt cttaagtgtt
     1141 attgccagca acataaacaa cagacggcaa aatgaataaa tgataacaaa gcagtaggct
     1201 taaataaacc taatttttat aggctgttct ctacaaccct caaacagtga ttagttttgt
     1261 acttataaac ttgccctttc attcatattt caagaaaatt ggttcagaag atctggatat
     1321 tctagcagtt gttcaagctc atggagggat cagtgacctg attccacaat gactaggcct
     1381 aatccagaaa ttagatgact gtcaacataa aaaggcacag cactcactag ctgccctata
     1441 tattttatta tattttacat atattatttt atttatttag ctctgagtgc tgtactttct
     1501 ggttaaagaa aactgcttac aacagctaac ctgtactacc tcaggctcag ggaatttgga
     1561 acaggtttgt ctggtttgtt tctttaacca tgcatgcttg ttttcaacta tggcaacaca
     1621 gtcacatggg acattacaga aatgatttgt cgatgacatg cgacttttct ttaataaagc
     1681 gcaaagatcc caaaaagcaa acttttaaca aaaatcatat aattatattt tcaatccagc
     1741 tttgtagcaa ctttgtgctg ctgttcactc agcaacagat agtcagtata aggtcagtgt
     1801 gtctcaaagc agtgccatct gtttcacaca ttgcgttcta tatataagtg tgctggttga
     1861 cacgacactg tataaggcct aggctaaaac acaaacaatg tagaatgaca ctgtgttttt
     1921 tttgtaaaca aatgttgttt ttggttaaac atctttgtga aaacatcctc ctgtcatgta
     1981 tttgctatat tcaaatgtta aacccgtgca gaatagaaca tatacaaaaa aaaacaacac
     2041 aacacatttt taaacattat taaatatcaa gtattgctgg cagttctgtt tctgttttac
     2101 agtacccttt gccacagttc tccgcttttc ctggtccaga ttccacaagt ctgattcacc
     2161 aatagcaaag cgaataaaca accaaagcag ccaatcactg cttgtagact gtcctgcgag
     2221 accggcccat tccagcacat tctggaaact tcctttatat gataattata aatacattta
     2281 aattattgat acaaaacatg taattcctag aacataacca tagcaatcat tagttttcag
     2341 ggtaattatg tatttttagg atttgactgc ggaaagatct ggtcatgtga cgtctcatga
     2401 acgtcacggc cctgggtttc tataaataca gtaggactct cgaccatcgg cagatttttc
     2461 gaagaagaag atcagtttca ggagccgtac tgttccgttt caacgcaaat attaacggta
     2521 agagcgaatt tcctagtttg ttttcatgcc attctttaaa accatagcgt attactttaa
     2581 ttatagtaaa ctttcgcttt ctttattaca agagacgttt tgtgttgatt ctccgcggac
     2641 attttcggtc agacaatcag aaaatgaccg cggaggacca gtaacttgca ttacacgtaa
     2701 gttaaatctt cgtgtattaa aatggttagg ttgttaacgt caaataggtt accgtgtttg
     2761 cgtgtgatca ggttggtttt gttagatttt tgtcagtatt tttaatttat ttgttttagt
     2821 ttatttattt tttttgctga atcatagttt gtgaacaaag aacccggatg ttacatacag
     2881 tacagccgcc atgttacaga gagttataac ttaatcattt taaaaataat tttgccttac
     2941 ttttagtttg tcatgttgag aaatgaggaa atgttaaaat gaggaaatat ccaattaatt
     3001 taatatatca aaataatcca tgattacaat gcactgaact ggagaaaatt aagatgtttt
     3061 ctagtgtcat gaaacaaatg taagagatgt acattgtaga tgttttatgt caagaattgg
     3121 ctagttgatg cagcatactg gcgatactca gttgtaataa cagtaacgtt acatgttaat
     3181 agactactga gtatgctgtt ctgtctatgt atgctctgta agctacgaga aggacttttt
     3241 taaacagtaa agggtgcaat atttttacaa attgaattaa ataaaggctg tctattaagt
     3301 aatatgcttg atatttttct tacttgatcg gaaataagaa aaaatataaa cgttgttgct
     3361 ctaaaaatcc tagttcagtt tagccaacca caaatacctt tttgttcctc caacagtttt
     3421 tttttcttct ctataatatt tggcagtcta tagtactcca aatgtttccc cacagtctaa
     3481 ctaattggta cagccaaaat catgacactt attgcaataa taattttggt tcattggcat
     3541 tgttgatagc ctgtgccact aatatggtcg attgatcatg cttcaggaag aaaactatat
     3601 tgtttgatgt aagattatta aatcttcacc tgcttccatt acaaactatt cccatcttat
     3661 tgaattctgg tatgtcttaa aggattagtt cacttcccaa atcaaaattt acttagcctt
     3721 ttttcatcca tgatcccttt ttttcatcat taatgaagaa attgtttttg aaaaagtttc
     3781 aagatttttt tctctatatt gtggagcttg ttagtttaaa attccaaaat gcaatatgtg
     3841 gcttcaaatg gttctaaatg atcccagtca aggaataaca gtcttatcta atgaaaccat
     3901 tagaccttta aaaataaaaa aaataaaagt atttattttt aaatgactga gtgattaagt
     3961 tgaatttcag cgtttcctta ctgtgtagaa gtccttcctt actggcccca ccctttggtt
     4021 ctctgccaat ctgctaccta atgtaatgtt gtggaacatt attattcttt atttcttaat
     4081 tttttatttt ttattttaaa acaatgtaaa ctgcacagat gtgcagtttg tttaaaatgg
     4141 ccaatgcttt ggaaatgcat gacataatta gatttcatga tgcacaaagc caaatctcag
     4201 agcttgtgca aaatgagcta tcatttcact aggtaagacc ctaaattttc atataggatc
     4261 atttggacaa ttttgctgca ggtaaaatgc attctatagt ccactgtcag ccattgtttt
     4321 ggatagtatt tatttttctc tacaagtata gtcaatagtt ttctattatt ttaaaggttt
     4381 gtaacattta agggtgacca aatgcaaagt aaaatttcat tttcgggtga actatctcgt
     4441 ttaacatggg agaagtgcaa aacatacatt attggctaga acattgtagt attttttaaa
     4501 tggaaatgtg tgattgctaa tcttactttg aatttgttta cagggatcca tggtgagcaa
     4561 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4621 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4681 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4741 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4801 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4861 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4921 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4981 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5041 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5101 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5161 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5221 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggaa gcggagctac
     5281 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctcccgggat
     5341 gtcgacgatg atggccacct acccaggcca tgaggataac ggcatgatac tcatggacac
     5401 aacctcgagt tcagcggaga aagaccgaac aaaagacgaa gctcctccag agaaggggcc
     5461 ggacaaatcc gacccgaccc agaaaccacc gtactcatac gtggcgttaa tcgcgatggc
     5521 aatccgcgag agctcggaga agcgtcttac gctgtccggt atataccagt acatcatcag
     5581 taagtttcca ttctacgaga agaacaagaa gggatggcag aacagcatcc gacacaacct
     5641 gtcactcaac gagtgcttca tcaaggtgcc tcgggagggc ggcggtgagc gaaagggcaa
     5701 ctactggacc ctggatcctg catgcgagga tatgtttgaa aagggcaact accggcgaag
     5761 acgacgaatg aaaagaccgt ttaggcctcc accgacccat ttccagccag gcaaatctct
     5821 ctttggcggt gaagggtacg gttacctctc cccgcccaaa tacctacagt ctggttttat
     5881 caataactca tggtcgcctg cacccatgtc atatacctcc tgtcaggtga gcagtgggag
     5941 tgtgagtccc gttaatatga aaggcttatc cgcaccatcg tcgtacaatc catactcgcg
     6001 cgtgcaaagt ataggtcttc ccagcatggt gaactcttac aacggcataa gccatcatca
     6061 tcaccaccac cacacgcatc ctcatgcgct cccgcacgcc cagcaactca gtcccgccac
     6121 ggctgcagca cctccggtga ccactggtaa cggaacagga ctgcagttcg cttgctcgcg
     6181 ccagcctgca gaactctcaa tgatgcactg ctcatattgg gatcacgaga gcaaacactc
     6241 tgcactacac gcgcggattg atatatagca tatgtaagta tacttgatat cgacatgata
     6301 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6361 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6421 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     6481 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
     6541 tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg
     6601 cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt
     6661 ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt
     6721 ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg
     6781 cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata
     6841 gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
     6901 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
     6961 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
     7021 gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag
     7081 caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
     7141 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7261 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7321 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttg
//
