LOCUS       62056_21440       10093 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..92)
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1382..1731
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     CDS             6382..7098
                     /codon_start=1
                     /label=EmGFP
                     /note="Emerald GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    7154..7275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8120..8150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8165..8186)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8474..9062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9236..10093)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtcccttt cgtcttcact
     1381 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     1441 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     1501 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     1561 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     1621 gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact tggatccatg
     1741 accgtcttaa cagacaaaag gaaaaaaggc agtggagctt ttatacaaga tgacgagaca
     1801 aaagaggttc tttcaagagc gctgagctat ttaaaatccg gctattccat tcattttaca
     1861 ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc ttgctaaaaa gagaaagcgt
     1921 cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca aagatttaat tggcgatttt
     1981 acgggataca cgagcaaaaa agtaatcgac cagtacgttc gttctgtcta taaaaaagat
     2041 gaacaggtga gtgaaaactg gcaggatggc cgattgcttg aagctgtaaa aaatggctat
     2101 acgctgattt acgacgaatt tactcgttct aagcctgcga cgaataatat ctttctatcg
     2161 atattagaag aaggcgtgct gccgctgtat ggagtaaaaa tgaccgatcc ttttgtgcgc
     2221 gtgcatcccg atttccgcgt catcttcaca agcaatccag ctgagtatgc cggcgtatat
     2281 gatacgcaag atgcgcttct cgacaggtta attaccatgt ttattgatta taaagacatc
     2341 gacagagaga cagcgatttt aacggagaaa acggacgtag aagaagatga agcgcgcaca
     2401 attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag acgaaaacag cagcggactt
     2461 agcctgcggg cttcgcttat gatcgctacc cttgccacgc agcaagacat tcctatcgat
     2521 ggaagtgacg aagattttca aacgttatgt atcgatattt tgcatcatcc gcttaccaaa
     2581 tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa tcattttaga agaatgtaag
     2641 aatatagaca ctgaagaaaa gaggagcttg aaaactgcaa caaattttag tcttttaaag
     2701 caggcaggag acgtcgagga aaaccctgga cccgtgatga gtgaaacaaa cgaaacaggt
     2761 atttatattt ttagcgccat tcaaacggat aaagacgaag aatttggcgc cgtggaagta
     2821 gaaggaacaa aagctgaaac atttttgatt cgctacaaag acgcggctat ggtagcagct
     2881 gaagtaccga tgaaaattta tcatcctaat cgccaaaatt tattaatgca tcaaaacgca
     2941 gtagcagcga ttatggacaa gaacgatacg gttattccaa tcagctttgg gaatgtattc
     3001 aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt atccgcagtt tgaaaagctg
     3061 tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag taattgggaa aaaagaatgg
     3121 cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag tatcagcatc cgtaaaagga
     3181 aaatcagaag cagccggtta ttatgagcgt attcaacttg gaggaatggc tcaaaagatg
     3241 tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt ctccgcttga agaagcagcg
     3301 gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc ttttaaacgc gtctttctta
     3361 attaaccgag aagatgaagc gaagtttgat gaaaaagtaa atgaagcgca tgaaaactgg
     3421 aaagacaaag ccgattttca ttacagcggt ccttggcctg cttataattt tgtgaacatt
     3481 cgcctaaaag tagaagagaa agcacctggc tcgggagcga ccaacttctc attactcaaa
     3541 caagccggag acgttgagga gaatccaggc cctgtgctgc acaagctcgt gaccgccccc
     3601 atcaacctgg tcgtgaagat cggcgagaag gtgcaggaag aggccgacaa gcagctgtac
     3661 gacctgccca ccatccagca gaagctgatc cagctgcaga tgatgttcga gctgggcgag
     3721 atccccgagg aagccttcca ggaaaaagag gacgaactgc tgatgagata cgagatcgcc
     3781 aagcggcgcg agattgagca gtgggaagaa ctgacccaga agcggaatga ggaaagcaac
     3841 gcagagaaat tgaacgccac taacttctcc ctgttgaaac aagcagggga tgtcgaagag
     3901 aatcccgggc caccgatggg agaattactg tatttatacg gtttaattcc aacaaaagaa
     3961 gcagcagcca tagagccgtt tccatcttat aaggggtttg acggagaaca ttcactgtac
     4021 ccaattgcgt ttgatcaggt gacggctgta gtttctaagc tggatgctga cacctattca
     4081 gaaaaagtga ttcaagaaaa aatggagcag gatatgagct ggctgcagga aaaagcattt
     4141 catcatcacg aaacggtagc cgctttgtac gaagaattta cgatcattcc attaaaattt
     4201 tgcaccattt ataaaggtga agaaagtctg caggcagcta ttgagattaa caaagaaaag
     4261 atagagaatt cactgacgct gcttcaagga aatgaagagt ggaatgtgaa aatttactgt
     4321 gatgatacag agcttaaaaa aggaatcagc gaaacgaatg aaagcgtgaa agcgaaaaaa
     4381 caagaaatta gtcacttatc accaggaaga cagttttttg aaaagaaaaa aatagatcag
     4441 ctgattgaaa aagaattaga gcttcacaaa aacaaagtgt gtgaagagat acatgacaag
     4501 ctaaaagaat tatcgcttta tgactctgtt aaaaagaatt ggagcaaaga cgtaactggc
     4561 gcagctgaac agatggcgtg gaacagcgtg tttcttctcc cgtctctgca gattactaag
     4621 ttcgtaaacg aaatagaaga gcttcagcaa aggcttgaaa ataaaggctg gaagtttgaa
     4681 gtgacgggac catggccgcc ctatcatttc tcgagctttg cgcgtgcttc acaaattagt
     4741 gcgaccaact tctcattact caaacaagcc ggagacgttg aggagaatcc aggccctgtg
     4801 agcctgaagc agagcatgga gaataaggat atcgccctga tcgacatcct cgacgtgatc
     4861 ctggacaagg gagtggccat caagggcgac ctgatcatct ctatcgccgg cgtggacctg
     4921 gtgtacctgg atctgagagt gctgatctcc agcgtggaaa ccctggtgca ggccaaagag
     4981 ggcaaccaca agcccatcac cagcgagcag ttcgacaagc agaaagagga gctgatggac
     5041 gccaccggcc agcccagcaa gtggacaaat cctctgggca gcggagcacc tggtagcggc
     5101 gccacgaatt tttcgttgtt gaagcaagct ggggatgttg aagagaaccc agggcctgtg
     5161 cagcccgtgt cccaggccaa cggcagaatc cacctggatc ccgatcaggc cgaacaggga
     5221 ctggcccagc tcgtgatgac cgtgatcgag ctgctgcggc agatcgtgga acggcacgcc
     5281 atgagaagag tggaaggcgg caccctgacc gacgagcaga tcgagaatct gggaatcgcc
     5341 ctgatgaacc tggaagagaa gatggacgag ctgaaagagg tgttcggact ggacgccgag
     5401 gacctgaaca tcgacctggg ccctctgggc agcctgctga cgataagatg gcaggaggca
     5461 acaaatttta gtcttttaaa gcaggcagga gacgtcgagg aaaaccctgg acccgtggcc
     5521 gtggaacaca acatgcagag cagcaccatc gtggacgtgc tggaaaagat cctggacaag
     5581 ggcgtcgtga tcgccgggga catcacagtg ggaatcgccg acgtggaact gctgaccatc
     5641 aagatccggc tgatcgtggc cagcgtggac aaggccaaag aaatcggcat ggattggtgg
     5701 gagaacgacc cctacctgag cagcaagggc gccaacaaca aggctctgga agaggaaaac
     5761 aagatgctgc acgagcggct gaaaacactg gaagagaaga tcgagacaaa gcgctataga
     5821 agcgccattg gcgcgaccaa cttctcatta ctcaaacaag ccggagacgt tgaggagaat
     5881 ccaggccctg tgagcaccgg ccccagcttc agcaccaagg acaacaccct ggaatacttc
     5941 gtgaaggcca gcaacaagca cggctttagc ctcgacatca gcctgaacgt gaatggggcc
     6001 gtgattagcg gcaccatgat cagcgccaaa gagtacttcg actacctgag cgagacattc
     6061 gaagagggca gcgaagtggc ccaggccctg tctgagcagt ttagcctggc tagcgaggcc
     6121 tccgagtcta atggcgaagc cgaggcccac ttcatccacc tgaagaacac caagatctac
     6181 tgcggcgaca gcaagagcac ccccagcaag ggcaagatct tctggcgcgg caagatcgcc
     6241 gaggtggacg gattcttcct gggaaaaatc agcgacgcca agtccaccag caagaagtcc
     6301 agctcattca aacgactcga cgccacgaat ttttcgttgt tgaagcaagc tggggatgtt
     6361 gaagagaacc cagggcctgt gatggtgagc aagggcgagg agctgttcac cggggtggtg
     6421 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6481 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6541 ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc
     6601 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6661 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6721 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6781 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
     6841 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
     6901 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6961 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     7021 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     7081 atggacgagc tgtacaagta agaattctac ctacgctatg tggatacgct gctttaatgc
     7141 ctttgtatca gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7261 catcaatgta tcttaatcta taacaagaaa atatatatat aataagttat cacgtaagta
     7321 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     7381 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     7441 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     7501 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     7561 acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt
     7621 ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt
     7681 gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc
     7741 attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac
     7801 gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc
     7861 cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac
     7921 agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac
     7981 gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag
     8041 ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8101 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8161 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8221 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8281 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10081 gttgaatact cat
//
