LOCUS       .                       4675 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             complement(772..783)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     terminator      911..1005
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             1064..1876
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      2098..2184
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2243..2264)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2280..3359)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3360..3437)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3629..3769
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3955..4543)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaggagaaat taaccatgag caagcatcac
      121 catcattcag gccatcacca taccggacac caccatcatt caggcagtca tcaccatacc
      181 ggcggatcat ccggaggtat gagctcagga tctaacggat ctacgggttc tggtactggt
      241 aatagcggga ccggaaccat gattaaagta aagactctga ccggcaagga aattgagatc
      301 gacatcgaac cgaccgacac catcgatcgt atcaaagaac gtgtggagga aaaagaaggc
      361 attcctccgg ttcagcagcg tctcatttac gcgggtaaac agcttgcaga tgacaaaacc
      421 gccaaagatt acaatatcga gggcggtagc gtactgcacc ttgttctggc cctgcgtggt
      481 ggcgccggta cctgcggatc ccaggtgcaa ttggtagagt gtgggggagg cttggtgcag
      541 cctggggggt ctctgagact ctcctgtgta gcctctggat tcaccttcag tagcgcctac
      601 atgagctggg tccgccaggc tccaggaaag gggcccgagt gggtctcaac tattagtaca
      661 ggtggtggta tcgtaaacta tgcagactcc gtgaagggcc gattcgccat ctccagagac
      721 aacgccaaga acaccctgta tctgcaaatg aacaaactga aacctgagga cacggccctc
      781 tattactgtg cgagcaataa aggcccgcat taccatagcg actattttga ttcgaatctg
      841 tatgacttct ggggccaggg gacccgggtc acagtctcat catgttaagc ttcttaatta
      901 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      961 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagccatga
     1021 aaataaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1081 ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1141 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     1201 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1261 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1321 ttttatccgt actcctgatg atgcttggtt actcacgact gcgatccccg gcaaaacagc
     1381 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1441 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1501 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     1561 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc acaaactttt
     1621 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1681 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     1741 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     1801 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     1861 gctcgatgag tttttctaag aattaattca tgggcaaata ttatacgcaa ggcgacaagg
     1921 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1981 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     2041 aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt gaggcatcaa
     2101 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2161 aacgctctcc tgagtaggac aaatccgccc tctagattac gtgcagtcga tgataagctg
     2221 tcaaacatga gaattgtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     2281 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2341 cggggagagg cggtttgcgt attgggggcc agggtggttt ttcttttcac cagtgagacg
     2401 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     2461 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     2521 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     2581 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     2641 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     2701 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     2761 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     2821 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     2881 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     2941 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     3001 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     3061 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     3121 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     3181 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     3241 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     3301 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     3361 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     3421 caccattcga tggtgtcgga atttcgggca gcgttgggtc ctggccacgg gtgcgcatga
     3481 tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag cagacaagcc cgtcagggcg
     3601 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     3661 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3721 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     3781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac
     4621 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agaaa
//
