LOCUS       V000975                 3907 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000975
VERSION     V000975
KEYWORDS    pTNT-B18R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR,
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi:
            10.1016/j.stem.2013.06.001.
   PUBMED   23910086
REFERENCE   2  (bases 1 to 3907)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date:
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..34
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        40..58
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      94..103
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..1155
                     /gene="OPG204"
                     /label="Soluble interferon alpha/beta receptor OPG204"
                     /note="Soluble interferon alpha/beta receptor OPG204 from
                     Vaccinia virus (strain Western Reserve). Accession#:
                     P25213"
     terminator      1197..1244
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1323..1778
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1845..1864)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     1964..1986
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2024..2042)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        2110..2214
                     /label="AmpR promoter"
     CDS             2215..3072
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3246..3834
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 taaggctaga gtacttaata cgactcacta taggctagca tttaggtgac actatagaat
       61 acaagctact tgttcttttt gcactcgaga attgccacca tgggcacgat gaaaatgatg
      121 gtacatatat atttcgtatc attattgtta ttgctattcc acagttacgc catagacatc
      181 gaaaatgaaa tcacagaatt cttcaataaa atgagagata ctctaccagc taaagactct
      241 aaatggttga atccagcatg tatgttcgga ggcacaatga atgatatagc cgctctagga
      301 gagccattca gcgcaaagtg tcctcctatt gaagacagtc ttttatcgca cagatataaa
      361 gactatgtgg ttaaatggga aaggctagaa aaaaatagac ggcgacaggt ttctaataaa
      421 cgtgttaaac atggtgattt atggatagcc aactatacat ctaaattcag taaccgtagg
      481 tatttgtgca ccgtaactac aaagaatggt gactgtgttc agggtatagt tagatctcat
      541 attagaaaac ctccttcatg cattccaaaa acatatgaac taggtactca tgataagtat
      601 ggcatagact tatactgtgg aattctttac gcaaaacatt ataataatat aacttggtat
      661 aaagataata aggaaattaa tatcgacgac attaagtatt cacaaacggg aaaggaatta
      721 attattcata atccagagtt agaagatagc ggaagatacg actgttacgt tcattacgac
      781 gacgttagaa tcaagaatga tatcgtagta tcaagatgta aaatacttac ggttataccg
      841 tcacaagacc acaggtttaa actaatacta gatccaaaaa tcaacgtaac gataggagaa
      901 cctgccaata taacatgcac tgctgtgtca acgtcattat tgattgacga tgtactgatt
      961 gaatgggaaa atccatccgg atggcttata ggattcgatt ttgatgtata ctctgtttta
     1021 actagtagag gcggtattac cgaggcgacc ttgtactttg aaaatgttac tgaagaatat
     1081 ataggtaata catataaatg tcgtggacac aactattatt ttgaaaaaac ccttacaact
     1141 acagtagtat tggagtaagc ggccgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaactag
     1201 cataacccct tggggcctct aaacgggtct tgaggggttt tttggatccg ggctggcgta
     1261 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1321 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1621 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1681 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1741 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     1801 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     1861 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1921 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1981 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     2041 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2101 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2161 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2221 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2281 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2341 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2401 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2461 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2521 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2581 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2641 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2701 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2761 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2821 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2881 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2941 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3001 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3061 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
     3901 cagatct
//
