LOCUS       40924_43518        4143 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway Entry vector pTK185, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Rozwadowski K, Yang W, Kagale S.
  TITLE     Homologous recombination-mediated cloning and manipulation of 
            genomic DNA regions using Gateway and recombineering systems
  JOURNAL   BMC Biotechnol. 8, 88 (2008)
  PUBMED    19014699
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Rozwadowski KL, Yang W, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2008) Molecular Genetics, Agriculture and 
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 
            0X2, Canada
REFERENCE   3  (bases 1 to 4143)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 8, 88 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2008) Molecular Genetics, Agriculture and Agri-Food Canada, 
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    67..166
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             200..2008
                     /codon_start=1
                     /gene="GUS"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /protein_id="ACL35319.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            200..2008
                     /gene="GUS"
                     /label=GUS
     protein_bind    complement(2084..2183)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2397..2768)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2787..2834)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     rep_origin      3112..3217
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      3202..3790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      3955..4041
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4133..4143
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat
      181 tcagtcgact ggatccacca tggtacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa
      241 actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg
      301 gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca
      361 gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt
      421 tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
      481 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
      541 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
      601 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
      661 caagaaaaag cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt
      721 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
      781 gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt
      841 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt
      901 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
      961 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
     1021 ggcagtgaag ggccaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
     1081 tcgtcatgaa gatgcggact tacgtggcaa aggattcgat aacgtgctga tggtgcacga
     1141 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
     1201 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
     1261 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
     1321 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
     1381 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
     1441 ggatacccgt ccgcaagtgc acgggaatat ttcgccactg gcggaagcaa cgcgtaaact
     1501 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     1561 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     1621 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     1681 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     1741 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     1801 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     1861 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     1921 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     1981 aaaaccgcag cagggaggca aacaatgaat caacaactct cctggcgcac catcgtcggc
     2041 tacagcctcg ggaattgcta ccgagctcgg taccttttac tagacccagc tttcttgtac
     2101 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     2161 agtcaaaata aaatcattat ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct
     2221 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     2281 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2341 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgaa ttctcagtcc
     2401 tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     2461 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     2521 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     2581 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     2641 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     2701 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     2761 ttggccatgg tttagttcct caccttgtcg tattatacta tgccgatata ctatgccgat
     2821 gattaattgt caacacgtgc tcgataaact tttgccattc tcaccggatt cagtcgtcac
     2881 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2941 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     3001 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3061 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     3121 ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt cagaccccgt
     3181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag
     3901 catggatctc ggggacgtct aactactaag cgagagtagg gaactgccag gcatcaaata
     3961 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     4021 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
     4081 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
     4141 atc
//
