LOCUS       .                       4497 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        165..183
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             247..1512
                     /gene="N"
                     /label="N"
                     /note="Nucleoprotein from Vesicular stomatitis Indiana
                     virus (strain San Juan). Accession#: P03521"
     terminator      1736..1783
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(1851..1869)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1890..1906)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1914..1930)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1938..1968)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1983..2004)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2292..2880)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3054..3911)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3912..4016)
                     /label="AmpR promoter"
     rep_origin      complement(4042..4497)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      181 aggacgaaga caaacaaacc attattatca ttaaaaggct caggagaaac tttaacagta
      241 atcaaaatgt ctgttacagt caagagaatc attgacaaca cagtcgtagt tccaaaactt
      301 cctgcaaatg aggatccagt ggaatacccg gcagattact tcagaaaatc aaaggagatt
      361 cctctttaca tcaatactac aaaaagtttg tcagatctaa gaggatatgt ctaccaaggc
      421 ctcaaatccg gaaatgtatc aatcatacat gtcaacagct acttgtatgg agcattaaag
      481 gacatccggg gtaagttgga taaagattgg tcaagtttcg gaataaacat cgggaaagca
      541 ggggatacaa tcggaatatt tgaccttgta tccttgaaag ccctggacgg cgtacttcca
      601 gatggagtat cggatgcttc cagaaccagc gcagatgaca aatggttgcc tttgtatcta
      661 cttggcttat acagagtggg cagaacacaa atgcctgaat acagaaaaaa gctcatggat
      721 gggctgacaa atcaatgcaa aatgatcaat gaacagtttg aacctcttgt gccagaaggt
      781 cgtgacattt ttgatgtgtg gggaaatgac agtaattaca caaaaattgt cgctgcagtg
      841 gacatgttct tccacatgtt caaaaaacat gaatgtgcct cgttcagata cggaactatt
      901 gtttccagat tcaaagattg tgctgcattg gcaacatttg gacacctctg caaaataacc
      961 ggaatgtcta cagaagatgt aacgacctgg atcttgaacc gagaagttgc agatgaaatg
     1021 gtccaaatga tgcttccagg ccaagaaatt gacaaggccg attcatacat gccttatttg
     1081 atcgactttg gattgtcttc taagtctcca tattcttccg tcaaaaaccc tgccttccac
     1141 ttctgggggc aattgacagc tcttctgctc agatccacca gagcaaggaa tgcccgacag
     1201 cctgatgaca ttgagtatac atctcttact acagcaggtt tgttgtacgc ttatgcagta
     1261 ggatcctctg ccgacttggc acaacagttt tgtgttggag ataacaaata cactccagat
     1321 gatagtaccg gaggattgac gactaatgca ccgccacaag gcagagatgt ggtcgaatgg
     1381 ctcggatggt ttgaagatca aaacagaaaa ccgactcctg atatgatgca gtatgcgaaa
     1441 agagcagtca tgtcactgca aggcctaaga gagaagacaa ttggcaagta tgctaagtca
     1501 gaatttgaca aatgacccta taattctcag acttaagacc ctcttgtggt ttttattttt
     1561 tatctggttt tgtggtcttc gtgggtcggc atggcatctc cacctcctcg cggtccgacc
     1621 tgggcatccg aaggaggacg tcgtccactc ggatggctaa gggaggggcc cccgcggggc
     1681 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     1741 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1801 atccggatcg agacctcgat actagtgcgg tggagctcca gcttttgttc cctttagtga
     1861 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1921 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1981 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2041 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2101 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2161 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2701 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4021 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4081 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4141 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4201 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4261 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4321 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4381 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4441 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgt
//
