LOCUS       .                       9673 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        165..183
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             330..6656
                     /gene="L"
                     /label="L"
                     /note="RNA-directed RNA polymerase L from Vesicular
                     stomatitis Indiana virus (strain Mudd-Summers). Accession#:
                     Q98776"
     terminator      6912..6959
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(7027..7045)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7066..7082)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7090..7106)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7114..7144)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7159..7180)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7468..8056)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8230..9087)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9088..9192)
                     /label="AmpR promoter"
     rep_origin      complement(9218..9673)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      181 aggacgaaga caaacaaacc attattatca ttaaaaggct gctagccaga ttcttcatgt
      241 ttggaccaaa tcaacttgtg ataccatgct caaagaggcc tcaattatat ttgagttttt
      301 aatttttatg aaaaaaacta acagcaatca tggaagtcca cgattttgag accgacgagt
      361 tcaatgattt caatgaagat gactatgcca caagagaatt cctgaatccc gatgagcgca
      421 tgacgtactt gaatcatgct gattacaacc tgaattctcc tctaattagt gatgatattg
      481 acaatttaat caggaaattc aattctcttc caattccctc gatgtgggat agtaagaact
      541 gggatggagt tcttgagatg ttaacgtcat gtcaagccaa tcccatccca acatctcaga
      601 tgcataaatg gatgggaagt tggttaatgt ctgataatca tgatgccagt caagggtata
      661 gttttttaca tgaagtggac aaagaggcag aaataacatt tgacgtggtg gagaccttca
      721 tccgcggctg gggcaacaaa ccaattgaat acatcaaaaa ggaaagatgg actgactcat
      781 tcaaaattct cgcttatttg tgtcaaaagt ttttggactt acacaagttg acattaatct
      841 taaatgctgt ctctgaggtg gaattgctca acttggcgag gactttcaaa ggcaaagtca
      901 gaagaagttc tcatggaacg aacatatgca ggattagggt tcccagcttg ggtcctactt
      961 ttatttcaga aggatgggct tacttcaaga aacttgatat tctaatggac cgaaactttc
     1021 tgttaatggt caaagatgtg attataggga ggatgcaaac ggtgctatcc atggtatgta
     1081 gaatagacaa cctgttctca gagcaagaca tcttctccct tctaaatatc tacagaattg
     1141 gagataaaat tgtggagagg cagggaaatt tttcttatga cttgattaaa atggtggaac
     1201 cgatatgcaa cttgaagctg atgaaattag caagagaatc aaggccttta gtcccacaat
     1261 tccctcattt tgaaaatcat atcaagactt ctgttgatga aggggcaaaa attgaccgag
     1321 gtataagatt cctccatgat cagataatga gtgtgaaaac agtggatctc acactggtga
     1381 tttatggatc gttcagacat tggggtcatc cttttataga ttattacact ggactagaaa
     1441 aattacattc ccaagtaacc atgaagaaag atattgatgt gtcatatgca aaagcacttg
     1501 caagtgattt agctcggatt gttctatttc aacagttcaa tgatcataaa aagtggttcg
     1561 tgaatggaga cttgctccct catgatcatc cctttaaaag tcatgttaaa gaaaatacat
     1621 ggcccacagc tgctcaagtt caagattttg gagataaatg gcatgaactt ccgctgatta
     1681 aatgttttga aatacccgac ttactagacc catcgataat atactctgac aaaagtcatt
     1741 caatgaatag gtcagaggtg ttgaaacatg tccgaatgaa tccgaacact cctatcccta
     1801 gtaaaaaggt gttgcagact atgttggaca caaaggctac caattggaaa gaatttctta
     1861 aagagattga tgagaagggc ttagatgatg atgatctaat tattggtctt aaaggaaagg
     1921 agagggaact gaagttggca ggtagatttt tctccctaat gtcttggaaa ttgcgagaat
     1981 actttgtaat taccgaatat ttgataaaga ctcatttcgt ccctatgttt aaaggcctga
     2041 caatggcgga cgatctaact gcagtcatta aaaagatgtt agattcctca tccggccaag
     2101 gattgaagtc atatgaggca atttgcatag ccaatcacat tgattacgaa aaatggaata
     2161 accaccaaag gaagttatca aacggcccag tgttccgagt tatgggccag ttcttaggtt
     2221 atccatcctt aatcgagaga actcatgaat tttttgagaa aagtcttata tactacaatg
     2281 gaagaccaga cttgatgcgt gttcacaaca acacactgat caattcaacc tcccaacgag
     2341 tttgttggca aggacaagag ggtggactgg aaggtctacg gcaaaaagga tggagtatcc
     2401 tcaatctact ggttattcaa agagaggcta aaatcagaaa cactgctgtc aaagtcttgg
     2461 cacaaggtga taatcaagtt atttgcacac agtataaaac gaagaaatcg agaaacgttg
     2521 tagaattaca gggtgctctc aatcaaatgg tttctaataa tgagaaaatt atgactgcaa
     2581 tcaaaatagg gacagggaag ttaggacttt tgataaatga cgatgagact atgcaatctg
     2641 cagattactt gaattatgga aaaataccga ttttccgtgg agtgattaga gggttagaga
     2701 ccaagagatg gtcacgagtg acttgtgtca ccaatgacca aatacccact tgtgctaata
     2761 taatgagctc agtttccaca aatgctctca ccgtagctca ttttgctgag aacccaatca
     2821 atgccatgat acagtacaat tattttggga catttgctag actcttgttg atgatgcatg
     2881 atcctgctct tcgtcaatca ttgtatgaag ttcaagataa gataccgggc ttgcacagtt
     2941 ctactttcaa atacgccatg ttgtatttgg acccttccat tggaggagtg tcgggcatgt
     3001 ctttgtccag gtttttgatt agagccttcc cagatcccgt aacagaaagt ctctcattct
     3061 ggagattcat ccatgtacat gctcgaagtg agcatctgaa ggagatgagt gcagtatttg
     3121 gaaaccccga gatagccaag tttcgaataa ctcacataga caagctagta gaagatccaa
     3181 cctctctgaa catcgctatg ggaatgagtc cagcgaactt gttaaagact gaggttaaaa
     3241 aatgcttaat cgaatcaaga caaaccatca ggaaccaggt gattaaggat gcaaccatat
     3301 atttgtatca tgaagaggat cggctcagaa gtttcttatg gtcaataaat cctctgttcc
     3361 ctagattttt aagtgaattc aaatcaggca cttttttggg agtcgcagac gggctcatca
     3421 gtctatttca aaattctcgt actattcgga actcctttaa gaaaaagtat catagggaat
     3481 tggatgattt gattgtgagg agtgaggtat cctctttgac acatttaggg aaacttcatt
     3541 tgagaagggg atcatgtaaa atgtggacat gttcagctac tcatgctgac acattaagat
     3601 acaaatcctg gggccgtaca gttattggga caactgtacc ccatccatta gaaatgttgg
     3661 gtccacaaca tcgaaaagag actccttgtg caccatgtaa cacatcaggg ttcaattatg
     3721 tttctgtgca ttgtccagac gggatccatg acgtctttag ttcacgggga ccattgcctg
     3781 cttatctagg gtctaaaaca tctgaatcta catctatttt gcagccttgg gaaagggaaa
     3841 gcaaagtccc actgattaaa agagctacac gtcttagaga tgctatctct tggtttgttg
     3901 aacccgactc taaactagca atgactatac tttctaacat ccactcttta acaggcgaag
     3961 aatggaccaa aaggcagcat gggttcaaaa gaacagggtc tgcccttcat aggttttcga
     4021 catctcggat gagccatggt gggttcgcat ctcagagcac tgcagcattg accaggttga
     4081 tggcaactac agacaccatg agggatctgg gagatcagaa tttcgacttt ttattccaag
     4141 caacgttgct ctatgctcaa attaccacca ctgttgcaag agacggatgg atcaccagtt
     4201 gtacagatca ttatcatatt gcctgtaagt cctgtttgag acccatagaa gagatcaccc
     4261 tggactcaag tatggactac acgcccccag atgtatccca tgtgctgaag acatggagga
     4321 atggggaagg ttcgtgggga caagagataa aacagatcta tcctttagaa gggaattgga
     4381 agaatttagc acctgctgag caatcctatc aagtcggcag atgtataggt tttctatatg
     4441 gagacttggc gtatagaaaa tctactcatg ccgaggacag ttctctattt cctctatcta
     4501 tacaaggtcg tattagaggt cgaggtttct taaaagggtt gctagacgga ttaatgagag
     4561 caagttgctg ccaagtaata caccggagaa gtctggctca tttgaagagg ccggccaacg
     4621 cagtgtacgg aggtttgatt tacttgattg ataaattgag tgtatcacct ccattccttt
     4681 ctcttactag atcaggacct attagagacg aattagaaac gattccccac aagatcccaa
     4741 cctcctatcc gacaagcaac cgtgatatgg gggtgattgt cagaaattac ttcaaatacc
     4801 aatgccgtct aattgaaaag ggaaaataca gatcacatta ttcacaatta tggttattct
     4861 cagatgtctt atccatagac ttcattggac cattctctat ttccaccacc ctcttgcaaa
     4921 tcctatacaa gccattttta tctgggaaag ataagaatga gttgagagag ctggcaaatc
     4981 tttcttcatt gctaagatca ggagaggggt gggaagacat acatgtgaaa ttcttcacca
     5041 aggacatatt attgtgtcca gaggaaatca gacatgcttg caagttcggg attgctaagg
     5101 ataataataa agacatgagc tatccccctt ggggaaggga atccagaggg acaattacaa
     5161 caatccctgt ttattatacg accacccctt acccaaagat gctagagatg cctccaagaa
     5221 tccaaaatcc cctgctgtcc ggaatcaggt tgggccaatt accaactggc gctcattata
     5281 aaattcggag tatattacat ggaatgggaa tccattacag ggacttcttg agttgtggag
     5341 acggctccgg agggatgact gctgcattac tacgagaaaa tgtgcatagc agaggaatat
     5401 tcaatagtct gttagaatta tcagggtcag tcatgcgagg cgcctctcct gagcccccca
     5461 gtgccctaga aactttagga ggagataaat cgagatgtgt aaatggtgaa acatgttggg
     5521 aatatccatc tgacttatgt gacccaagga cttgggacta tttcctccga ctcaaagcag
     5581 gcttggggct tcaaattgat ttaattgtaa tggatatgga agttcgggat tcttctacta
     5641 gcctgaaaat tgagacgaat gttagaaatt atgtgcaccg gattttggat gagcaaggag
     5701 ttttaatcta caagacttat ggaacatata tttgtgagag cgaaaagaat gcagtaacaa
     5761 tccttggtcc catgttcaag acggtcgact tagttcaaac agaatttagt agttctcaaa
     5821 cgtctgaagt atatatggta tgtaaaggtt tgaagaaatt aatcgatgaa cccaatcccg
     5881 attggtcttc catcaatgaa tcctggaaaa acctgtacgc attccagtca tcagaacagg
     5941 aatttgccag agcaaagaag gttagtacat actttacctt gacaggtatt ccctcccaat
     6001 tcattcctga tccttttgta aacattgaga ctatgctaca aatattcgga gtacccacgg
     6061 gtgtgtctca tgcggctgcc ttaaaatcat ctgatagacc tgcagattta ttgaccatta
     6121 gcctttttta tatggcgatt atatcgtatt ataacatcaa tcatatcaga gtaggaccga
     6181 tacctccgaa ccccccatca gatggaattg cacaaaatgt ggggatcgct ataactggta
     6241 taagcttttg gctgagtttg atggagaaag acattccact atatcaacag tgtttagcag
     6301 ttatccagca atcattcccg attaggtggg aggctgtttc agtaaaagga ggatacaagc
     6361 agaagtggag tactagaggt gatgggctcc caaaagatac ccgaatttca gactccttgg
     6421 ccccaatcgg gaactggatc agatctctgg aattggtccg aaaccaagtt cgtctaaatc
     6481 cattcaatga gatcttgttc aatcagctat gtcgtacagt ggataatcat ttgaaatggt
     6541 caaatttgcg aagaaacaca ggaatgattg aatggatcaa tagacgaatt tcaaaagaag
     6601 accggtctat actgatgttg aagagtgacc tacacgagga aaactcttgg agagattaaa
     6661 aaatcatgag gagactccaa actttaagta tgaaaaaaac tttgatcctt aagaccctct
     6721 tgtggttttt attttttatc tggttttgtg gtcttcgtgg gtcggcatgg catctccacc
     6781 tcctcgcggt ccgacctggg catccgaagg aggacgtcgt ccactcggat ggctaaggga
     6841 ggggcccccg cggggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     6901 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     6961 tgaaaggagg aactatatcc ggatcgagac ctcgatacta gtgcggtgga gctccagctt
     7021 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     7081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9181 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     9241 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     9301 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     9361 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     9421 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     9481 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     9541 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     9601 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     9661 ctacagggcg cgt
//
