LOCUS       .                       2914 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     RBS             complement(521..543)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(575..593)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1104..1692)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1908..2012
                     /label="AmpR promoter"
     CDS             2013..2870
                     /label="AmpR"
                     /note="β-lactamase"
ORIGIN
        1 aattctcatg tttgacagct tatcatcgat gataagcttg gatggaacat ctgtcagcag
       61 atctcaagaa actgggagca aagatatttc ttaggcttca ggttcgtagt cttgataccc
      121 ttcctcagaa ggcatttcat aagcctcatt gtcaggatcc acaggcatat cttccagaat
      181 tccttcctgt ggggctcctt cttcattctt gcccaactgg tcctttttga caaagccagt
      241 ggctgctgca atgctccctg ctccctccac tgtcttctgg gctactgctg tcacacccgt
      301 caccactgct cctccaacat ttgtcacttg ctctttggtc ttctcagcca ctgttgccac
      361 accatgcacc actccctcct tggttttgga gcctacatag agaacaccct cttttgtctt
      421 tcctgctgct tctgccacac cctgtttggt tttctcagca gcagccacaa ctccctcctt
      481 ggcctttgaa agtcctttca tgaatacatc catatgtata tctccttctt aaagttaaac
      541 aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcga
      601 agtctatcag aagttcgaat cgctgggcct cgcgcgtttc ggtgatgacg gtgaaaacct
      661 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      721 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
      781 gtcacgtagc gatagcggag tgtatatact ggcttaacta tgcggcatca gagcagattg
      841 tactgagagt gcaccataca gatctgtatg cggtgtgaaa taccgcacag atgcgtaagg
      901 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1261 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     1321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1501 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1801 ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1861 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1921 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1981 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2041 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2101 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2161 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2221 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2281 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2341 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2401 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2461 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2521 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2581 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2641 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2701 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2761 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2821 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2881 accaagttta ctcatatata ctttagattg attt
//
