LOCUS       40924_40987        9275 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSoup, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Hellens RP.
  TITLE     An update on the pGreen vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   Hellens RP.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2007) Horticulture and Food Research Institute of 
            New Zealand (HortResearch), 120 Mt. Albert Road, Auckland, NZ 1003, 
            New Zealand
REFERENCE   3  (bases 1 to 9275)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2007) Horticulture and Food Research Institute of New 
            Zealand (HortResearch), 120 Mt. Albert Road, Auckland, NZ 1003, New 
            Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            618..727
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             760..1128
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1258..1969
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    2442..2498
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(2505..2523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2541..2557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2565..2581)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2589..2619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2634..2655)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2902..2930
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2978..4165
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             4667..5812
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             7196..8164
                     /codon_start=1
                     /label=RepA
                     /note="replication protein of plasmid pSa"
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
     CDS             8562..9209
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
       61 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
      121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      481 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
      541 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
      601 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
      661 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
      721 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
      781 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
      841 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
      901 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
      961 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     1021 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     1081 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     1141 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     1201 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     1261 cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     1321 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     1381 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     1441 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     1501 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     1561 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     1621 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     1681 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     1741 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     1801 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     1861 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     1921 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     1981 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     2041 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     2101 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     2161 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     2221 gtgctcgtgt tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     2281 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     2341 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     2401 ttgacaatac tgataagata atatatcttt tatatagaag ataattcgag ctcggtaccc
     2461 ggggatcctc tagagtcgac ctgcaggcat gcaagcttga gtattctata gtgtcaccta
     2521 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2581 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2641 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2701 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2881 aacatgaatt aattctcatg tttgacagct tatcatcgat tagctttaat gcggtagttt
     2941 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     3001 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     3061 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     3121 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
     3181 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
     3241 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggccggcat
     3301 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     3361 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     3421 cgtggccggg ggactgttgg gcgccatctc cttacatgca ccattccttg cggcggcggt
     3481 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     3541 gcgccgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     3601 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     3661 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     3721 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     3781 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     3841 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     3901 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     3961 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     4021 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     4081 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     4141 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
     4201 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     4261 ccttgtatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     4321 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagatgtg
     4381 gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca agtgttttgg
     4441 ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg
     4501 caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga ccgacttcat
     4561 tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc aggaataagg
     4621 gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga atcggacgtt
     4681 tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg aggatgccga
     4741 aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc
     4801 gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc
     4861 cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg
     4921 tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga agcgaaaaac
     4981 cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt tgctgaaaca
     5041 cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc cgtggccgga
     5101 cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa
     5161 gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa
     5221 gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt ggcagcaggt
     5281 gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt tctacgagct
     5341 ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg aggaatgcct
     5401 gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc acctggaatc
     5461 ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca
     5521 ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca cgaaattcat
     5581 atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact atttcagctc
     5641 gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg gatcggattc
     5701 cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag
     5761 cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac gctagggcct
     5821 tgtggggtca gttccggctg ggggttcagc agccagcgct ttactggcat ttcaggaaca
     5881 agcgggcact gctcgacgca cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc
     5941 tacgaactgc cgataaacag aggattaaaa ttgacaattg tgattaaggc tcagattcga
     6001 cggcttggag cggccgacgt gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa
     6061 gctccagaga tgttcgggtc cgtttacgag cacgaggaga aaaagcccat ggaggcgttc
     6121 gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca tcgacggcga gatcattggg
     6181 ctgtcggtct tcaaacagga ggacggcccc aaggacgctc acaaggcgca tctgtccggc
     6241 gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg
     6301 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
     6361 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
     6421 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
     6481 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
     6541 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
     6601 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     6661 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     6721 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     6781 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     6841 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     6901 cccagatcct agaggatcga gcggcatttc attcaggcac gtaccaccga ggcgctacaa
     6961 gtcgccagag agcgcggcaa gacgctcggg cgacccaagg gcagcaaatc gagcgccttg
     7021 aagctggaca gccgtattga tgaagtacag gcatacgtga accttggctt gccgcaaagt
     7081 cgcgcagccg agttgttagg cgtcagccct cacaccttgc gcctgttcat caaacgccgg
     7141 aacatcaaac ccacaaacac tagaccaacc atcaccatgc cggggaggga acaacatgcc
     7201 taagaacaac aaagcccccg gccatcgtat caacgagatc atcaagacga gcctcgcgct
     7261 cgaaatggag gatgcccgcg aagctggctt agtcggctac atggcccgtt gccttgtgca
     7321 agcgaccatg ccccacaccg accccaagac cagctacttt gagcgcacca atggcatcgt
     7381 caccttgtcg atcatgggca agccgagcat cggcctgccc tacggttcta tgccgcgcac
     7441 cttgcttgct tggatatgca ccgaggccgt gcgaacgaaa gaccccgtgt tgaaccttgg
     7501 ccggtcgcaa tcggaatttc tacaaaggct cggaatgcac accgatggcc gttacacggc
     7561 cacccttcgc aatcaggcgc aacgcctgtt ttcatccatg atttcgcttg ccggcgagca
     7621 aggcaatgac ttcggcattg agaacgtcgt cattgccaag cgcgcttttc tattctggaa
     7681 tcccaagcgg ccagaagatc gggcgctatg ggatagcacc ctcaccctca caggcgattt
     7741 cttcgaggaa gtcacccgct caccggttcc tatccgaatc gactacctgc acgccttgcg
     7801 gcagtctccg cttgcgatgg acatttacac gtggctgacc tatcgcgtgt tcctgttgcg
     7861 ggccaagggc cgccccttcg tgcaaatccc ttgggtcgcc ctgcaagcgc aattcggctc
     7921 atcctatggc agccgcgcac gcaactcgcc cgaactggac gataaggccc gagagcgggc
     7981 agagcgggca gcactcgcca gcttcaaata caacttcaaa aagcgcctac gcgaagtgtt
     8041 gattgtctat cccgaggcaa gcgactgcat cgaagatgac ggcgaatgcc tgcgcatcaa
     8101 atccacacgc ctgcatgtca cccgcgcacc cggcaagggc gctcgcatcg gcccccctcc
     8161 gacttgacca ggccaacgct acgcttggct tggtcaagga tcgatctggc gccggccagc
     8221 gagacgagca agattggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg
     8281 caggcaaatt gcaccaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg
     8341 ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg
     8401 acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtatgttggg tttcacgtct
     8461 ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag
     8521 ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga
     8581 atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga
     8641 cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga
     8701 acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt
     8761 cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca
     8821 ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg
     8881 caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg
     8941 ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg
     9001 aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc
     9061 tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt
     9121 tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga
     9181 acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac
     9241 tcactacagc agagccatgt agacaacatc ccctc
//
