LOCUS       pSecTag_B.        5170 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector for the secretion of C-terminally 
            6xHis-tagged proteins (replaced by pSecTag2 B). For other reading 
            frames, use pSecTag A or pSecTag C.
ACCESSION   .
VERSION     .
KEYWORDS    pSecTag B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     905..967
                     /label=Ig-kappa leader
                     /note="leader sequence from mouse immunoglobulin kappa 
                     light
                     chain"
     misc_feature    970..1092
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1093..1122
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1138..1155
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1184..1408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1454..1882
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1896..2225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2273..2320
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2339..2710
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2843..2976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3013..3029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3037..3053)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3061..3091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3106..3127)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3415..4003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4177..5034)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5035..5139)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      961 tggtgacgcg gcccagccgg ccaggcgcgc gcgccgtacg aagcttggta ccgagctcgg
     1021 atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag
     1081 tctagagggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
     1141 catcatcatc atcattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     1201 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1261 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1321 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1381 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     1441 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     1621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1801 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     1861 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     1921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2101 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2161 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2221 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     2281 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     2341 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     2401 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     2461 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     2521 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     2581 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     2641 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     2701 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     2761 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     2821 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     3001 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3061 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3601 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5161 acctgacgtc
//
