LOCUS       .                       5107 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(5106..5107,1..17))
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        805..909
                     /label="AmpR promoter"
     CDS             910..1767
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1941..2529
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2715..2857)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             3094..4743
                     /label="luciferase"
                     /note="firefly luciferase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
      121 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
      181 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
      241 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      301 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      361 tgagcgcttg tttcggcgtg ggtatggtgg caggcccgtg gccgggggac tgttgggcgc
      421 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      481 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      661 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      721 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      781 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      841 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      901 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      961 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1021 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1081 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1141 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1201 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1261 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1321 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1381 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1441 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1501 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1561 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1621 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1681 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1741 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     2341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2701 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2761 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatata
     2821 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     2881 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2941 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcccag
     3001 ctggcttatc gaaattaata cgactcacta taaggagacc ggaattcact agtattcttc
     3061 tggtccccac agactcagag agaacccgcc accatggaag acgccaaaaa cataaagaaa
     3121 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
     3181 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
     3241 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
     3301 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
     3361 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
     3421 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
     3481 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
     3541 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
     3601 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
     3661 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
     3721 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
     3781 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     3841 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     3901 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
     3961 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     4021 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     4081 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     4141 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     4201 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     4261 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     4321 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     4381 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     4441 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     4501 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     4561 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     4621 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     4681 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
     4741 gtgtaagctg gagcctcggt ggcctagctt cttgcccctt gggcctcccc ccagcccctc
     4801 ctccccttcc tgcacccgta cccccgtggt ctttgaataa agtctgagtg ggcggcgggc
     4861 gtgatgcgaa agctgacccg ggcgtgatgc gaaagctgac ccgctctgac cgaaaggcgt
     4921 gatgagcgct ctgaccgaaa ggcgtgatga gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4981 aaagggtacc actaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5041 aaaaaaaaaa aaaaaaaaaa aaaaaggatc ctctagagtc gacctgcagg catgcaagct
     5101 tgtattc
//
