LOCUS       .                       3211 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    56..112
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        504..532
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             721..1251
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     oriT            1451..1559
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(1573..2232)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2233..3004)
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     terminator      complement(3119..3205)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
      301 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      361 acagtaatac aaggggtgtt atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      421 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggacgc acaccgtgga
      481 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca
      541 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      601 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg
      661 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      721 atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaggt
      781 ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt caaatccatg
      841 cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta ctcccaacat
      901 cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat cgcgcttgct
      961 gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc caggtttgag
     1021 cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca ccggaggcag
     1081 ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct tggtgcttat
     1141 gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta tacaaagttg
     1201 ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac ctaacaattc
     1261 gttcaagccg agatcggctt cccgtaatta attggaccgc ggtccgcgcg ttgtcctttt
     1321 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     1381 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     1441 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     1501 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt
     1561 aggccggccc taccggcgcg gcagcgttac ccgtgtcggc ggctccaacg gctcgccatc
     1621 gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc
     1681 ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg
     1741 cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg
     1801 gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc
     1861 ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt
     1921 gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc
     1981 ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga
     2041 aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg
     2101 cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt
     2161 gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga
     2221 ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc
     2281 aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc
     2341 aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg
     2401 ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc
     2461 tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg
     2521 ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg
     2581 ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg
     2641 gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc
     2701 aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact
     2761 tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa
     2821 aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg
     2881 gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc
     2941 tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg
     3001 ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag
     3061 ggcgaagccc ggagagcaag cccgtagggg gggcgcgccc agctgtctag ggcggcggat
     3121 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3181 tcgactgagc ctttcgtttt atttgatgcc t
//
