LOCUS       40924_38653        4790 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6.masP.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 4790)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        441..821
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    823..863
                     /note="multiple cloning site I; MCS I"
     intron          866..2214
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2217..2260
                     /note="multiple cloning site II; MCS II"
     terminator      2264..2516
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(2569..2585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2593..2609)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2617..2647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2662..2683)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2971..3559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3733..4590)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4591..4695)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggttgagat ttttcaaatc agtgcgcaag acgtgacgta agtatctgag
      481 ctagttttta tttttctact aatttggtcg tttatttcgg cgtgtaggac atggcaaccg
      541 ggcctgaatt tcgcgggtat tctgtttcta ttccaacttt ttcttgatcc gcagccatta
      601 acgacttttg aatagatacg ctgacacgcc aagcctcgct agtcaaaagt gtaccaaaca
      661 acgctttaca gcaagaacgg aatgcgcgtg acgctcgcgg tgacgccatt tcgccttttc
      721 agaaatggat aaatagcctt gcttcctatt atatcttccc aaattaccaa tacattacac
      781 tagcatctga atttcataac caatctcgat acaccaaatc gaccatggtc cggactcaga
      841 tctcgagctc aagcttcgaa ttcgtgtaag aatttcttat gttacattat tacattcaac
      901 gttttatctt aattggctct tcatttgatt gaaatttgac aattatttct tgtttttttt
      961 tttgtcacac tctttttggg ttggggtggc cgacgaattg tgggaaggta gaaagagggg
     1021 aggacttttg ttatactcca ttagtaatta ctgtttccgt ttcaatttat gtgacaatat
     1081 ttccttttta gtcggttcca aaagaaaatg tcagcattat aaacaattta attttgaaat
     1141 tacaattttg ccattaataa aatgatttac aaccacaaaa gtatctatga gcctgtttgg
     1201 gtgggcttat aagcagctta ttttaagtgg cttataagtc aaaaagtgac antttttgag
     1261 aagttagaaa atcctaactt ctcaaaaagt agcttttaag ccacttatga cttataagtc
     1321 caaaaatttt taagttacca aacatatatt aatgggttta taagcttata agccactttt
     1381 aagctcaccc aaacgggttc tatgtctcac tttagactac aaattttaaa agtcttcatt
     1441 tatttcttaa tctccgtggc gagtnaaact ataacacata aagtgaaacg gagggaataa
     1501 gatggagtca taaactaatc caaatctata ctctctccgt taatttgttt tttagtttga
     1561 tttggtacat taataaaaca gatttttcga aggttataaa cacagacaga tgtttcccag
     1621 cgagctagca aaattccaag atttctgtcg aaaattcgtg tgtttctagc tagtacttga
     1681 tgttatcttt aaccttttag taattttttg tccttttctt tctatttttc atcttacaat
     1741 gaattatgag caagttcctt aagtagcatc acacgtgaga tgttttttat gatattgact
     1801 aaatccaatc tttaccattc cttaactagt aaaatacaac acatgttaat tgatacattg
     1861 cttaacactg aggttagaaa attttagaaa ttagttgtcc aaatgctttg aaattagaaa
     1921 tctttaatcc cttatttttt tttaaaatgt tttttctcac tccaaagaaa gagaaactga
     1981 catgaaagct caaaagatca tgaatcttac taactttgtg gaactaaatg tacatcagaa
     2041 tgtttctgac atgtgaaaat gaaagctctt aattttcttc ttttatttat tgagggtttt
     2101 tgcatgctat gcattcaatt tgagtacttt aaagcaccta taaacactta cttacacttg
     2161 ccttggagtt tatgttttag tgttttcttc acatcttttt tggtcaattt gcaggtctgc
     2221 agtcgacggt accgcgggcc cgggatccac ctagtctaga aatcttggac tcccatgttg
     2281 gcaaaggcaa ccaaacaaac aatgaatgat ccgctcctgc atatggggcg gtttgagtat
     2341 ttcaactgcc atttgggctg aattgaagac atgctcctgt cagaaattcc gtgatcttac
     2401 tcaatattca gtaatctcgg ccaatatcct aaatgtgcgt ggctttatct gtctttgtat
     2461 tgtttcatca attcatgtaa cgtttgcttt tcttatgaat tttcaaataa attatcagat
     2521 cgcggccgca cccataatac ccataatagc tgtttgccag taatcatggt catagctgtt
     2581 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2641 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2701 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2761 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     3181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4621 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4681 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4741 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
