LOCUS       62056_19185        4654 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            387..496
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      611..999
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        complement(2102..2132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2147..2168)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2266..2293)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2385..2471)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2533..3324)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(3742..3789)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3809..4465)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4466..4568)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tttgggtgaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
       61 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
      121 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata
      181 ggaactaagg aggatattca catggaccat ggctaattcc catacgcgtc aattcgaggg
      241 ggatcaattc cgttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
      301 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
      361 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
      421 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
      481 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
      541 accaagccaa ccaggaaggg cagcccacct atcacggaat tgatccggcc acgatgcgtc
      601 cggcgtagag gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca
      661 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
      721 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
      781 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
      841 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt
      901 aacggttgtg gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa
      961 agcaattttg agtgacacag gaacacttaa cggctgacat gggaattccc ctccaccgcg
     1021 gtggcggccg cttattcaac atagttccct tcaagagcga tacaacgatt ataacgacct
     1081 tccaattttt tgataccatt ttggtagtac tccttcggtt ttgcctcaaa ataggcctca
     1141 gtttcggcga tcacctcttc attgcagcca aattttttcc ctgcgagcat ccttttgagg
     1201 tctgagaaca agaaaaagtc gctgggggcc agatctggag aatacggcgg gtggggaagc
     1261 aattcgaagc ccaattcatg aatttttgcc atcgttctca atgacttgtg gcacggtgcg
     1321 ttgtcttggt ggaacaacac ttttttcttc ttcatgtggg gccgttttgc cgcgatttcg
     1381 accttcaaac gctccaataa cgccatataa tagtcactgt tgatggtttt tcccttctca
     1441 agataatcga taaaaattat tccatgcgca tcccaaaaaa cagaggccat tactttgcca
     1501 gcggactttt gagtctttcc acgcttcgga gacggttcac cggtcgctgt ccactcagcc
     1561 gactgtcgat tggactcagg agtgtagtga tggagccatg tttcatccat tgtcacatat
     1621 cgacggaaaa actcgggtgt attacgagtt aacagctgca aacaccgctt agaatcatca
     1681 acacgtcgtt gtttttggtc aaatgtgagc tcgcgcggca cccatttcgc acagagcttc
     1741 cgcatatcca aatattgatg aatgatatga ccaacacgtt cctttgatat ctttaaggcc
     1801 tctgctatct cgatcaactt cattttacgg tcattcaaaa tcattttgtg gatttttttg
     1861 atgttttcgt cggtaaccac ctctttcggg cgtccactgc gttcaccgtc ctccgtgctc
     1921 atttcaccac gcttgaattt tgcataccaa tcaattattg ttgatttccc tggggcagag
     1981 tccggaaact cattatcaag ccaagttttt gcttccactg tattttttcc cttcagaaaa
     2041 cagtatttta tcaaaacacg aaattccttt ttttccatat gtatccgctc acaattccac
     2101 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2161 tcacattaat tgcgttgcgc tcactggatc ctaacaggtt ggatgataag tccccggtct
     2221 tcgtatgccg tcttctgctt ggcgcgccct gcaggtagaa acgcaaaaag gccatccgtc
     2281 aggatggcct tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     2341 cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     2401 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     2461 cgttttattt gatgcctggc agttccctac tctcgcattc tagagtcatt tcgaacccca
     2521 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     2581 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     2641 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     2701 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     2761 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     2821 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     2881 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     2941 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     3001 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     3061 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     3121 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     3181 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     3241 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     3301 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct
     3361 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     3421 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     3481 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     3541 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     3601 tttctgcgga ctggctttct acgtgttccg cttcctcaat tgctcgaggg cgcgccaagc
     3661 agaagacggc atacgaagac cggggactta tcatccaacc tgttaggtac cgaggacgcg
     3721 tgtgtaggct ggagctgctt cgaagttcct atactttcta gagaatagga acttcggaat
     3781 aggaacttca tttaaatggc gcgccttacg ccccgccctg ccactcatcg cagtactgtt
     3841 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     3901 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     3961 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4021 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4081 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4141 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4201 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgtaat tccggatgag
     4261 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4321 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4381 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4441 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacaact
     4501 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     4561 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     4621 caccaggatt tattatctgc gaagactacg ccgt
//
