LOCUS       40924_36773        7266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRESQ101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Expression enhancement of a rice polyubiquitin gene promoter
  JOURNAL   Plant Mol. Biol. 60 (2), 225-239 (2006)
  PUBMED    16429261
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Sivamani E, Starmer J, Qu R.
  TITLE     Sequence analysis of rice rubi3 promoter gene expression cassettes 
            for improved transgene expression
  JOURNAL   Plant Sci. 177 (6), 549-556 (2009)
REFERENCE   3  (bases 1 to 7266)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2008) Crop Science, North Carolina State 
            University, 101, Derieux Place, Raleigh, NC 27695, USA
REFERENCE   4  (bases 1 to 7266)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2006"; volume: "60"; issue: "2"; pages: "225-239"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant 
            Sci."; date: "2009"; volume: "177"; issue: "6"; pages: "549-556"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2008) Crop Science, North Carolina State University, 101, 
            Derieux Place, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      325..1108
                     /gene="rubi3"
                     /regulatory_class="promoter"
     exon            1109..1175
                     /gene="rubi3"
                     /number=1
     intron          1176..2315
                     /gene="rubi3"
                     /number=1
     CDS             2322..4130
                     /label=GUS
                     /note="beta-glucuronidase"
     terminator      4135..4386
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4395..4411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4624..5079
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5361..5465
                     /label=AmpR promoter
     CDS             5466..6323
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6497..7085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agtaacggcc gccagtgtgc tggaattcgg cttagtcgac
      301 ccacccaacc ccatatcgac agaggatgtg aagaacaggt aaatcacgca gaagaaccca
      361 tctctgatag cagctatcga ttagaacaac gaatccatat tgggtccgtg ggaaatactt
      421 actgcacagg aaggggggcg atctgacgag gccccgccac cggcctcgac ccgaggccga
      481 ggccgacgaa gcgccggcga gtacggcgcc gcggcggcct ctgcccgtgc cctctgcgcg
      541 tgggagggag aggccgcggt ggtgggggcg cgcgcgcgcg cgcgcgcagc tggtgcggcg
      601 gcgcgggggt cagccgccga gccggcggcg acggaggagc agggcggcgt ggacgcgaac
      661 ttccgatcgg ttggtcagag tgcgcgagtt gggcttagcc aattaggtct caacaatcta
      721 ttgggccgta aaattcatgg gccctggttt gtctaggccc aatatcccgt tcatttcagc
      781 ccacaaatat ttccccagag gattattaag gcccacacgc agcttatagc agatcaagta
      841 cgatgtttcc tgatcgttgg atcggaaacg tacggtcttg atcaggcatg ccgacttcgt
      901 caaagagagg cggcatgacc tgacgcggag ttggttccgg gcaccgtctg gatggtcgta
      961 ccgggaccgg acacgtgtcg cgcctccaac tacatggaca cgtgtggtgc tgccattggg
     1021 ccgtacgcgt ggcggtgacc gcaccggatg ctgcctcgca ccgccttgcc cacgctttat
     1081 atagagaggt tttctctcca ttaatcgcat agcgagtcga atcgaccgaa ggggaggggg
     1141 agcgaagctt tgcgttctct aatcgcctcg tcaaggtaac taatcaatca cctcgtccta
     1201 atcctcgaat ctctcgtggt gcccgtctaa tctcgcgatt ttgatgctcg tggtggaaag
     1261 cgtaggagga tcccgtgcga gttagtctca atctctcagg gtttcgtgcg attttagggt
     1321 gatccacctc ttaatcgagt tacggtttcg tgcgatttta gggtaatcct cttaatctct
     1381 cattgattta gggtttcgtg agaatcgagg tagggatctg tgttatttat atcgatctaa
     1441 tagatggatt ggttttgaga ttgttctgtc agatggggat tgtttcgata tattacccta
     1501 atgatgtgtc agatggggat tgtttcgata tattacccta atgatgtgtc agatggggat
     1561 tgtttcgata tattacccta atgatggata ataagagtag ttcacagtta tgttttgatc
     1621 ctgccacata gtttgagttt tgtgatcaga tttagtttta cttatttgtg cttagttcgg
     1681 atgggattgt tctgatattg ttccaataga tgaatagctc gttaggttaa aatctttagg
     1741 ttgagttagg cgacacatag tttatttcct ctggatttgg attggaattg tgttcttagt
     1801 ttttttcccc tggatttgga ttggaattgt gtggagctgg gttagagaat tacatctgta
     1861 tcgtgtacac ctacttgaac tgtagagctt gggttctaag gtcaatttaa tctgtattgt
     1921 atctggctct ttgcctagtt gaactgtagt gctgatgttg tactgtgttt ttttacccgt
     1981 tttatttgct ttactcgtgc aaatcaaatc tgtcagatgc tagaactagg tggctttatt
     2041 ctgtgttctt acatagatct gttgtcctgt agttacttat gtcagttttg ttattatctg
     2101 aagatatttt tggttgttgc ttgttgatgt ggtgtgagct gtgagcagcg ctcttatgat
     2161 taatgatgct gtccaattgt agtgtagtat gatgtgattg atatgttcat ctattttgag
     2221 ctgacagtac cgatatcgta ggatctggtg ccaacttatt ctccagctgc ttttttttac
     2281 ctatgttaat tccaatcctt tcttgcctct tccagcccgg gatgttacgt cctgtagaaa
     2341 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     2401 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
     2461 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
     2521 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     2581 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     2641 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     2701 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     2761 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     2821 atgccggaat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     2881 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     2941 tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     3001 gacaaggcac tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     3061 gttatctcta tgaactcgaa gtcacagcca aaagccagac agagtctgat atctacccgc
     3121 ttcgcgtcgg catccggtca gtggcagtga agggccaaca gttcctgatt aaccacaaac
     3181 cgttctactt tactggcttt ggtcgtcatg aagatgcgga cttacgtggc aaaggattcg
     3241 ataacgtgct gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc
     3301 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     3361 tggtgattga tgaaactgct gctgtcggct ttcagctgtc tttaggcatt ggtttcgaag
     3421 cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag
     3481 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     3541 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     3601 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     3661 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     3721 gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     3781 aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     3841 tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     3901 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3961 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     4021 agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     4081 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgagctcgtt
     4141 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     4201 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4261 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4321 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     4381 tagatcggga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     4561 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4621 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4801 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5041 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5101 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5221 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5281 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5341 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5401 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5461 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5521 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5581 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5641 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5701 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5761 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5821 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5881 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5941 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6001 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6061 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6121 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6181 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6241 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6301 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6361 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6421 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6481 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6601 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7021 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7081 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7141 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7201 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7261 cggaag
//
