LOCUS       .                       6949 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    complement(115..236)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        481..838
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             889..1821
                     /label="hRluc"
                     /note="Renilla luciferase"
     CDS             1873..2661
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2728..2776
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        2926..3030
                     /label="AmpR promoter"
     CDS             3031..3888
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      complement(4049..4637)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    4753..5036
                     /label="cer region"
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     promoter        5202..5233
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             5244..6893
                     /label="luciferase"
                     /note="firefly luciferase"
ORIGIN
        1 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
       61 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
      121 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      181 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      241 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
      301 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
      361 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
      421 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
      481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      841 gcttgattct tctgacacaa cagtctcgaa ccaaaggctg gagccaccat ggcttccaag
      901 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
      961 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     1021 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     1081 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     1141 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     1201 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     1261 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     1321 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     1381 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     1441 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     1501 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     1561 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     1621 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     1681 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     1741 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     1801 cgcgtgctga agaacgagca gaccggtggt gggagcggag gtggcggatc aggtggcgga
     1861 ggctccggag ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2701 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2761 gttggttttt tgtgtgaatc gatagcgata atctttgcgc ttgcgttttc ccttgtccag
     2821 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     2881 gtaatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2941 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3001 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc
     3241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3421 actgcggcca acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg
     3481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg
     3901 accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca
     3961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4621 gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4741 tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga
     4801 aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg
     4861 gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt
     4921 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg
     4981 cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata
     5041 aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatctc gagctcaagc
     5101 ttcgaatttg gccggtacct gaaactaggg taaagttcat gaaactaggg taaagttcat
     5161 gaaactaggg taaagttcaa gatctgcaga agcttagaca ctagagggta tataatggaa
     5221 gctcgacttc cagggatccc accatggaag atgccaaaaa cattaagaag ggcccagcgc
     5281 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
     5341 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
     5401 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
     5461 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     5521 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     5581 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     5641 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     5701 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     5761 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     5821 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     5881 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     5941 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     6001 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     6061 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     6121 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     6181 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     6241 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     6301 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     6361 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     6421 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     6481 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     6541 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     6601 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     6661 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     6721 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     6781 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     6841 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaattct
     6901 agttgtttaa acgagctcgc tagcctcgag tctagagtcg acctgcagg
//
