LOCUS       .                       6818 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2088..3737
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(3781..3902)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4321..4909)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5083..5940)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5941..6045)
                     /label="AmpR promoter"
     rep_origin      6072..6527
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6658..6706
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6720..6811
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
ORIGIN
        1 ggtaccggtt ttttgtttgt tttttttaat ttagggttgc acctgcggca catggaagtt
       61 cccaggctag gggtcgaatc ggagctgcag acaccagcct acaccacagc cacagccacg
      121 caggatctaa gccatgtctg cgacctacac ccaagctccc ggcaacactg gatcctttaa
      181 ccgactgcac aaggccaagg gacgaacccg catcttcatg gataccggtc gggcgcctaa
      241 cccactgagc cacaatggaa attcctggaa gacagtgttt tgacctgacc cagtcaagct
      301 aaatccaaaa agctaaatgc accactgtaa cctagttaat aaattggttt ctcacagagg
      361 gatgggctgg caactctgcg atcaccctgt gtacgtggcg aggattgaac aaatacataa
      421 atgtagtgtc cataatgaga gggaaacttt tcgctgtctg aaaaagatct aacaataagg
      481 caaggaggaa agctagactc aacccttgtg gtgctggatt agaactggag gttatccgta
      541 tgaagccgtg gttttgattg ctcaagacag acgcaggcat gcatatccac acagtgttta
      601 cgtgtacata acggttcgtg tgtacgggtc tatgtgcaca caccccttgt ttactgaagg
      661 agcctcggag caacgtgaac ccaagagcag tgagctcacc tgccccccag atcttgtcta
      721 aacaccattc cgcctcgaag ggaaccaggg cccctcggag aagagctgat tccctgtagg
      781 tactcttcca acaggtcaaa gtcatgaaaa acaagggaaa attcaggtac ttctccaaac
      841 cagaggaggc tgaggagaga aatgaccatt aaatgcgacg tggaagcttc gattggatcc
      901 ttgaagaaaa aatagacaaa ctattgaaag tcaaataatg tctgtactgc agctaatgtt
      961 attgcaccaa ggttaatttt cagatttgga tcatcttact ctggttatct gaaatgttca
     1021 ttttattttt ttatttttta ttttcatttt tatttattta tttagtcttt ttagggccac
     1081 atcatcggca tatggaggtt cccaggctag gggtccaatc ggagctgtag ccaccggccc
     1141 acaccagagc cacagcaacg caggatccga gccgcgtctg caacctacac cacagctcac
     1201 ggcaacgccg gatcgttaac ccactgagca agggcaggga ccgaacccgc aacctcctgg
     1261 ttcctagtcg gattcgttaa ccactgcgcc atgacgggaa ctccactcac ttttttttag
     1321 aaagaaaaag aaatccgcag gctaaggcga tacccgttca agaactgaca tgaggatctc
     1381 ccgttgtggc tccgcaggaa cgaacccgac gagtatccct gaggacgtga gttcaatccg
     1441 agcatacacg gattcaagtc ccatctccgc cacttcccag ctgccacctt cgagccacct
     1501 accgctgttg tgcctcagtc ttcttgccag ttagctggtg gtcatcacct cggccccttg
     1561 gaacagtgag gacaactcaa taaattaatc cacgcgcagc atttagtgca gggccgcaca
     1621 cacagcaggc gtttaatcag tgccagggag agtatgcaaa ggaggccgaa gagacagcag
     1681 gagaaagagc ctctgattag tacggaattc cagacaacct gttttctcct ctaccccgcc
     1741 agcacgctcc ccaatgttaa aaaggcaata aatacacact aaatcatagg tattgacagt
     1801 attacagaga aaaagggaat tttaaaaatt ggtaaaagct ctgcctggcg tgtaaaacca
     1861 atcagatttt ctaacgagtc aaaaaaaatt tccccctaaa aagacacatg agtgtactcg
     1921 gcccggtagg gaaggaatac agaacttttt ggcaagaact actttcgttt tcaagataaa
     1981 acgaaactat cttgcaaact ctttgcctgg attgcactgg ccaataaaaa cctctgaatc
     2041 tgtcctttgc ataagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca
     2101 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     2161 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     2221 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     2281 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     2341 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     2401 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     2461 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     2521 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     2581 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     2641 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     2701 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     2761 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     2821 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     2881 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     2941 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     3001 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     3061 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     3121 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     3181 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     3241 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     3301 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     3361 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     3421 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     3481 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     3541 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     3601 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     3661 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     3721 gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     3781 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3841 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3901 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3961 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
     4021 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     4081 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4501 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4741 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6061 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6481 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt
     6541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     6601 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     6661 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     6721 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     6781 agtgcaagtg caggtgccag aacatttctc tatcgata
//
