LOCUS       .                       4804 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        177..208
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             241..954
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     polyA_signal    complement(1001..1122)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        1316..1673
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1704..2495
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2523..2571
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2898..3486)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3689..4546)
                     /label="AmpR"
                     /note="β-lactamase"
     misc_feature    4713..4804
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg tacctgagct cgctagcctc gagggcctaa ctggccggta
       61 ccgctagcct cgatagagga aaattgctga ttgcaagagg aaaattgctg attgcagcgc
      121 gtagatctgc agaagcttag acacaagatc tggcctcggc ggccaagctt agacactaga
      181 gggtatataa tggaagctcg acttccagct tggcaatccg gtactgttgg taaagccacc
      241 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      301 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      361 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      421 gtcactactt tcacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
      481 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      541 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      601 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      661 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      721 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      781 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      841 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      901 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatagtaa
      961 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
     1021 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1081 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1141 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     1201 tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt gggcgctctt ccgctgatct
     1261 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttagct accttctgag
     1321 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     1381 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     1441 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1501 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1561 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     1621 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcg
     1681 attcttctga cactagcgcc accatgatcg aacaagacgg cctccatgct ggcagtcccg
     1741 cagcttgggt cgaacgcttg ttcgggtacg actgggccca gcagaccatc ggatgtagcg
     1801 atgcggccgt gttccgtcta agcgctcaag gccggcccgt gctgttcgtg aagaccgacc
     1861 tgagcggcgc cctgaacgag cttcaagacg aggctgcccg cctgagctgg ctggccacca
     1921 ccggcgtacc ctgcgccgct gtgttggatg ttgtgaccga agccggccgg gactggctgc
     1981 tgctgggcga ggtccctggc caggatctgc tgagcagcca ccttgccccc gctgagaagg
     2041 tttctatcat ggccgatgca atgcggcgcc tgcacaccct ggaccccgct acctgcccct
     2101 tcgaccacca ggctaagcat cggatcgagc gtgctcggac ccgcatggag gccggcctgg
     2161 tggaccagga cgacctggac gaggagcatc agggcctggc ccccgctgaa ctgttcgccc
     2221 gactgaaagc ccgcatgccg gacggtgagg acctggttgt cacacacgga gatgcctgcc
     2281 tccctaacat catggtcgag aatggccgct tctccggctt catcgactgc ggtcgcctag
     2341 gagttgccga ccgctaccag gacatcgccc tggccacccg cgacatcgct gaggagcttg
     2401 gcggcgagtg ggccgaccgc ttcttagtct tgtacggcat cgcagctccc gacagccagc
     2461 gcatcgcctt ctaccgcttg ctcgacgagt tcttttaatg atctagaacc ggtcatggcc
     2521 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
     2581 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     2641 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     2701 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2761 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2821 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3061 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3301 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3541 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     3661 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     3721 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     3781 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     3841 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     3901 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     3961 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     4021 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     4081 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     4141 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     4201 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     4261 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     4321 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     4381 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     4441 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     4501 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     4561 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
     4621 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
     4681 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     4741 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     4801 ctct
//
