LOCUS       V017142                 7948 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017142
VERSION     V017142
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1138..1378)
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        1617..1996
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1997..2200
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2245..2263
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2289..3545
                     /gene="pylS"
                     /label="Pyrrolysine--tRNA ligase"
                     /note="Pyrrolysine--tRNA ligase from Methanosarcina
                     barkeri. Accession#: Q6WRH6"
     polyA_signal    3581..3805
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3851..4279
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4293..4622
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4689..5480
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5657..5790
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5825..5841)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5849..5865)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5873..5903)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5918..5939)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6227..6815)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6989..7846)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(7847..7948,1..3))
                     /label="AmpR promoter"
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctga cgtcgacaaa aaacggaaac cccgggaatc
       61 gaacccggct gaacggattt agagtccgtt cggtctccct gaccaggttt ccggtgtttc
      121 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
      181 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      241 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      301 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
      361 cttcctgccc ctcgacaaaa aacggaaacc ccgggaatcg aacccggctg aacggattta
      421 gagtccgttc ggtctccctg accaggtttc cggtgtttcg tcctttccac aagatatata
      481 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      541 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      601 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      661 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccc tcgacaaaaa
      721 acggaaaccc cgggaatcga acccggctga acggatttag agtccgttcg gtctccctga
      781 ccaggtttcc ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
      841 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
      901 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
      961 tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
     1021 tcatgggaaa taggccctct tcctgcccct cgacaaaaaa cggaaacccc gggaatcgaa
     1081 cccggctgaa cggatttaga gtccgttcgg tctccctgac caggtttccg gtgtttcgtc
     1141 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
     1201 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
     1261 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct
     1321 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt
     1381 cctgcccctc gagtctagac ccgatcccct atggtgcact ctcagtacaa tctgctctga
     1441 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     1501 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     1561 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     1621 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1681 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1741 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1801 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1861 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1921 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1981 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2041 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2101 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2161 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     2221 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     2281 gcgccaccat ggacaagaaa cccctggacg tgctgatctc cgccaccggc ctgtggatga
     2341 gcagaaccgg caccctgcac aagatcaagc accacgaggt gtccagaagc aagatctaca
     2401 tcgagatggc ctgcggcgac cacctggtcg tgaacaacag cagaagctgc cggaccgcca
     2461 gagccttccg gcaccacaag tacagaaaga cctgcaagcg gtgccgggtg tccgacgagg
     2521 atatcaacaa cttcctgacc agaagcaccg agagcaagaa cagcgtgaaa gtgcgggtgg
     2581 tgtccgcccc caaagtgaag aaagccatgc ccaagagcgt gtccagagcc cccaagcccc
     2641 tggaaaattc cgtgtccgcc aaggccagca ccaacaccag cagatccgtg cccagccctg
     2701 ccaagagcac ccccaatagc tctgtgcctg cctctgcccc tgcccccagc ctgacaagat
     2761 cccagctgga cagagtggaa gccctgctga gccccgagga caagatcagc ctgaacatgg
     2821 ccaagccctt cagagagctg gaacccgagc tcgtgacccg gcggaagaac gacttccagc
     2881 ggctgtacac caacgacaga gaggactacc tgggcaagct ggaacgggac atcaccaagt
     2941 tcttcgtgga ccggggcttc ctggaaatca agagccccat cctgatcccc gccgagtacg
     3001 tggaacggat gggcatcaac aacgacaccg agctgagcaa gcagatcttc cgggtggaca
     3061 aaaacctgtg cctgaggccc atgctggccc ccaccctggc caactacctg cggaaactgg
     3121 accggatcct gcctggcccc atcaagatct tcgaagtggg cccctgctac cggaaagaga
     3181 gcgacggcaa agagcacctg gaagagttta caatggtcaa cttctgccag atgggcagcg
     3241 gctgcaccag agagaatctg gaagctctga tcaaagagtt cctggactat ctggaaatcg
     3301 acttcgagat cgtgggcgac agctgcatgg tgttcggcga caccctggac atcatgcacg
     3361 gcgacctgga actgagcagc gctgtcgtgg gacccgtgtc cctggataga gagtggggca
     3421 tcgacaagcc ctggatcgga gccggcttcg gcctggaaag actgctgaaa gtgatgcacg
     3481 gcttcaagaa catcaagcgg gcctcccgca gcgagagcta ctacaacggc atctccacca
     3541 acctgtgagc ggccgcttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     3601 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3661 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3721 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3781 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3841 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4261 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4561 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4621 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     4681 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4741 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4801 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4861 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4921 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4981 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5041 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5101 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5161 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5221 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5281 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5341 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5401 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5461 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5521 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5581 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5641 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5761 actcatcaat gtatcttatc atgtctgtat accggtcctc tagctagagc ttggcgtaat
     5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5881 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7921 tttagaaaaa taaacaaata ggggttcc
//
