LOCUS       V013029                 6416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013029
VERSION     V013029
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    334..350
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             420..1136
                     /label="Cycle 3 GFP"
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     misc_feature    2685..2825
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3011..3599)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3773..4630)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4631..4735)
                     /label="AmpR promoter"
     CDS             5115..5849
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     terminator      6163..6249
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6268..6295
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      6371..6405
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 aagaacgttg ctctagaata attctacaca gcccagtcca gactattcgg cactgaaatt
       61 atgggaattc tacacagccc agtccagact attcggcact gaaattatgg gtgaagtggt
      121 caagacctca ctaggcacct taaaaatagc gcaccctgaa gaagatttat ttgaggtagc
      181 ccttgcctac ctagcttcca agaaagatat cctaacagca caagagcgga aagatgtttt
      241 gttctacatc cagaacaacc tctgctaaaa ttcctgaaaa attttgcaaa aagttgttga
      301 ctttatctac aaggtgtggc ataatgtgtg gaattgtgag cggataacaa ttaagcttaa
      361 ggaggtgatc tagagtcgag ggtaccagcg ctatcgatcg gccggaagga gatatacata
      421 tggctagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      481 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg
      541 gaaagcttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      601 ttgtcactac tttgacctat ggtgttcaat gcttttcccg ttatccggat catatgaaac
      661 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      721 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      781 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca
      841 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      901 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag
      961 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1021 acctgtcgac acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
     1081 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagccc tacaaataat
     1141 gaattcgagc actagtgcag cccgcctaat gagcgggctt ttttccatgc aagctaattc
     1201 ggtggaaacg aggtcatcat ttccttccga aaaaacggtt gcatttaaat cttacatatg
     1261 taatactttc aaagactaca tttgtaagat ttgatgtttg agtcggctga aagatcgtac
     1321 gtaccaatta ttgtttcgtg attgttcaag ccataacact gtagggatag tggaaagagt
     1381 gcttcatctg gttacgatca atcaaatatt caaacggagg gagacgattt tgatgaaacc
     1441 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     1501 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aagtggaagc ggcgatggcg
     1561 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     1621 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     1681 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     1741 gtcgaagcct gtaaaacggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     1801 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     1861 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     1921 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     1981 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     2041 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     2101 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     2161 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     2221 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     2281 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     2341 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     2401 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2521 cgcaattaat gtgagttagg atcctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2581 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2641 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     2701 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     2761 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2821 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3421 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3721 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3781 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3841 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3901 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3961 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4021 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4081 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4141 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4201 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4261 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4321 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4381 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4441 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4501 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4561 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4621 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4741 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4801 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt gatcctctag cacaaaaaga
     4861 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
     4921 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
     4981 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta
     5041 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
     5101 gaaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     5161 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     5221 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     5281 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     5341 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     5401 aaattgttgg gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     5461 aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     5521 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     5581 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     5641 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     5701 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     5761 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     5821 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     5881 aagttacacg ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc
     5941 aaggagctaa agaggtccct agactctaga attcggatcc cgggaggaaa taattctatg
     6001 agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta
     6061 ggtatactac tgacagcttc caaggagcta aagaggtccc tagactctag acccggggat
     6121 ctctgcagtc ggatctggta atgactctct agcttgaggc atcaaataaa acgaaaggct
     6181 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     6241 aggacaaatc cgccgctcta gctaagcaga aggccatcct gacggatggc ctttttgcgt
     6301 ttctacaaac tcttgttaac tctagagctg cctgccgcgt ttcggtgatg aagatccgat
     6361 gattaattaa ttcagaacgc tcggttgccg ccgggcgttt tttatgcagc aatggc
//
