LOCUS       V004418                 5254 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004418
VERSION     V004418
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Minton NP.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   J. Microbiol. Methods 78 (1), 79-85 (2009)
   PUBMED   19445976
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Pennington OJ, Heap JT, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The
            University of Nottingham, University Park, Nottingham NG7 2RD,
            United Kingdom
REFERENCE   3  (bases 1 to 5254)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5254)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2009"; volume: "78"; issue: "1"; pages:
            "79-85"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Centre for Biomolecular Sciences, The University of
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /note="terminator derived from CD0164 gene of Clostridium
                     difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(199..215)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      363..404
                     /note="terminator derived from fdx gene of Clostridium
                     pasteurianum"
                     /regulatory_class="terminator"
     CDS             925..1980
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label="repA"
                     /protein_id="ACR43886.1"
                     /translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNIE
                     RTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRKEE
                     NKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQKEKY
                     ITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRERWLE
                     LWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKALKGKQV
                     LVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTEDEKEEL
                     NQDLIDEIEID"
     gene            925..1980
                     /gene="repA"
                     /label="repA"
     CDS             complement(2071..2574)
                     /codon_start=1
                     /product="putative plasmid replication protein"
                     /label="putative plasmid replication protein"
                     /note="orf2"
                     /protein_id="ACR43887.1"
                     /translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYVG
                     PEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFATVT
                     IKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFKKEL
                     KTKN"
     CDS             2898..3518
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      3698..4286
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            4607..4716
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             4749..5117
                     /label="traJ"
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
      121 ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg
      181 cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcta
      361 gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgttctgaa
      421 tccttagcta atggttcaac aggtaactat gacgaagata gcaccctgga taagtctgta
      481 atggattcta aggcatttaa tgaagacgtg tatataaaat gtgctaatga aaaagaaaat
      541 gcgttaaaag agcctaaaat gagttcaaat ggttttgaaa ttgattggta gtttaattta
      601 atatattttt tctattggct atctcgatac ctatagaatc ttctgttcac ttttgttttt
      661 gaaatataaa aaggggcttt ttagcccctt ttttttaaaa ctccggagga gtttcttcat
      721 tcttgatact atacgtaact attttcgatt tgacttcatt gtcaattaag ctagtaaaat
      781 caatggttaa aaaacaaaaa acttgcattt ttctacctag taatttataa ttttaagtgt
      841 cgagtttaaa agtataattt accaggaaag gagcaagttt tttaataagg aaaaattttt
      901 ccttttaaaa ttctatttcg ttatatgact aattataatc aaaaaaatga aaataaacaa
      961 gaggtaaaaa ctgctttaga gaaatgtact gataaaaaaa gaaaaaatcc tagatttacg
     1021 tcatacatag cacctttaac tactaagaaa aatattgaaa ggacttccac ttgtggagat
     1081 tatttgttta tgttgagtga tgcagactta gaacatttta aattacataa aggtaatttt
     1141 tgcggtaata gattttgtcc aatgtgtagt tggcgacttg cttgtaagga tagtttagaa
     1201 atatctattc ttatggagca tttaagaaaa gaagaaaata aagagtttat atttttaact
     1261 cttacaactc caaatgtaaa aagttatgat cttaattatt ctattaaaca atataataaa
     1321 tcttttaaaa aattaatgga gcgtaaggaa gttaaggata taactaaagg ttatataaga
     1381 aaattagaag taacttacca aaaggaaaaa tacataacaa aggatttatg gaaaataaaa
     1441 aaagattatt atcaaaaaaa aggacttgaa attggtgatt tagaacctaa ttttgatact
     1501 tataatcctc attttcatgt agttattgca gttaataaaa gttattttac agataaaaat
     1561 tattatataa atcgagaaag atggttggaa ttatggaagt ttgctactaa ggatgattct
     1621 ataactcaag ttgatgttag aaaagcaaaa attaatgatt ataaagaggt ttacgaactt
     1681 gcgaaatatt cagctaaaga cactgattat ttaatatcga ggccagtatt tgaaattttt
     1741 tataaagcat taaaaggcaa gcaggtatta gtttttagtg gattttttaa agatgcacac
     1801 aaattgtaca agcaaggaaa acttgatgtt tataaaaaga aagatgaaat taaatatgtc
     1861 tatatagttt attataattg gtgcaaaaaa caatatgaaa aaactagaat aagggaactt
     1921 acggaagatg aaaaagaaga attaaatcaa gatttaatag atgaaataga aatagattaa
     1981 agtgtaacta tactttatat atatatgatt aaaaaaataa aaaacaacag cctattaggt
     2041 tgttgttttt tattttcttt attaattttt ttaattttta gtttttagtt cttttttaaa
     2101 ataagtttca gcctcttttt caatattttt taaagaagga gtatttgcat gaattgcctt
     2161 ttttctaaca gacttaggaa atattttaac agtatcttct tgcgccggtg attttggaac
     2221 ttcataactt actaatttat aattattatt ttctttttta attgtaacag ttgcaaaaga
     2281 agctgaacct gttccttcaa ctagtttatc atcttcaata taatattctt gacctatata
     2341 gtataaatat atttttatta tatttttact tttttctgaa tctattattt tataatcata
     2401 aaaagtttta ccaccaaaag aaggttgtac tccttctggt ccaacatatt tttttactat
     2461 attatctaaa taatttttgg gaactggtgt tgtaatttga ttaatcgaac aaccagttat
     2521 acttaaagga attataacta taaaaatata taggattatc tttttaaatt tcattattgg
     2581 cctccttttt attaaattta tgttaccata aaaaggacat aacgggaata tgtagaatat
     2641 ttttaatgta gacaaaattt tacataaata taaagaaagg aagtgtttgt ttaaatttta
     2701 tagcaaacta tcaaaaatta gggggataaa aatttatgaa aaaaaggttt tcgatgttat
     2761 ttttatgttt aactttaata gtttgtggtt tatttacaaa ttcggccggc cagtgggcaa
     2821 gttgaaaaat tcacaaaaat gtggtataat atctttgttc attagagcga taaacttgaa
     2881 tttgagaggg aacttagatg gtatttgaaa aaattgataa aaatagttgg aacagaaaag
     2941 agtattttga ccactacttt gcaagtgtac cttgtaccta cagcatgacc gttaaagtgg
     3001 atatcacaca aataaaggaa aagggaatga aactatatcc tgcaatgctt tattatattg
     3061 caatgattgt aaaccgccat tcagagttta ggacggcaat caatcaagat ggtgaattgg
     3121 ggatatatga tgagatgata ccaagctata caatatttca caatgatact gaaacatttt
     3181 ccagcctttg gactgagtgt aagtctgact ttaaatcatt tttagcagat tatgaaagtg
     3241 atacgcaacg gtatggaaac aatcatagaa tggaaggaaa gccaaatgct ccggaaaaca
     3301 tttttaatgt atctatgata ccgtggtcaa ccttcgatgg ctttaatctg aatttgcaga
     3361 aaggatatga ttatttgatt cctattttta ctatggggaa atattataaa gaagataaca
     3421 aaattatact tcctttggca attcaagttc atcacgcagt atgtgacgga tttcacattt
     3481 gccgttttgt aaacgaattg caggaattga taaatagtta acttcaggtt tgtctgtaac
     3541 taaaaacaag tatttaagca aaaacatcgt agaaatacgg tgttttttgt taccctaagt
     3601 ttaaactcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg cagggccccc tgcttcgggg
     4501 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     4561 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     4621 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     4681 tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg
     4741 gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt
     4801 actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc
     4861 tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg
     4921 agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga
     4981 aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc
     5041 tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc
     5101 gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt
     5161 gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac
     5221 atcaccgacg agcaaggcaa gaccgatcgg gccc
//
