LOCUS       62056_17390        5824 bp DNA     circular SYN 30-JUN-2020
DEFINITION  Cloning vector pMSx230, complete sequence.
ACCESSION   MT267331
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P, 
            Fussenegger M.
  TITLE     Phosphoregulated orthogonal signal transduction in mammalian cells
  JOURNAL   Nat Commun 11 (1), 3085 (2020)
  PUBMED    32555187
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Scheller L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment 
            AI), Station 19, Lausanne 1015, Switzerland
REFERENCE   3  (bases 1 to 5824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station
            19, Lausanne 1015, Switzerland"
FEATURES             Location/Qualifiers
     source          1..5824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    158..291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(328..344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(352..368)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(376..406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(421..442)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(730..1315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1489..2346)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2347..2451)
                     /label=AmpR promoter
     misc_feature    2502
                     /label=Promoter Region
                     /note="Promoter Region"
     misc_feature    2520..2723
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
     promoter        2773..2791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2820..2825
                     /label=Kozak+ATG
                     /note="Kozak+ATG"
     CDS             2835..3152
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     misc_feature    3180..3839
                     /label=DcuS truncation
                     /note="DcuS truncation"
     polyA_signal    3906..4130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4176..4604
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4618..4947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5014..5805
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
       61 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
      121 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
      181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
      301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1261 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2461 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc cacgcgtggt accctcgagg
     2521 cgatctgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2581 cctaactccg cccagttccg cccattctcc gccccatcgc tgactaattt tttttattta
     2641 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2701 tggaggccta ggcttttgca aaagagctct ctggctaact agagaaccca ctgcttactg
     2761 gcttatcgaa attaatacga ctcactatag ggagacccaa gctgttcgaa gcggaattca
     2821 ccatgactag tgctgttcag gttgaaacca ttagcccggg agatggccgt acatttccga
     2881 aacgtggcca gacatgtgtt gttcactata ccggcatgct ggaggatggt aaaaaatttg
     2941 atagcagccg tgaccgtaat aaaccgttca aattcatgct gggcaaacag gaagtcattc
     3001 gcggttggga agaaggtgtc gcacagatga gtgtgggtca acgtgccaaa ctgacgatta
     3061 gtcctgacta tgcctatgga gcaacaggtc atccagggat tattccgcct cacgctactc
     3121 tggtctttga tgtggaactg ctgaaactgg aagctagtgg cggcggcggc agtgctagta
     3181 aaactgaagt acgtaaactg atgcagcgac tcgacggtct ggtcaactat gctgacgcac
     3241 ttcgtgaacg atcccacgaa tttatgaata aattgcatgt gattctcgga ttattgcatc
     3301 tgaagagtta taagcagttg gaagattaca ttctcaaaac agccaataac tatcaggaag
     3361 agattggctc tctgctgggt aagatcaaat ctccggttat cgctggtttt ttaatcagca
     3421 agattaaccg cgcgaccgat ttaggccata cgctgatttt aaacagtgaa agccagctgc
     3481 cagacagcgg cagtgaggac caggtcgcga cgctgattac cacgttggga aatctgatag
     3541 aaaacgcgct ggaggcatta gggccggaac ccggaggcga aattagcgta acattgcact
     3601 accgtcacgg ctggctgcac tgtgaagtta atgatgatgg accggggatc gcacccgata
     3661 aaatcgatca catttttgac aaaggtgtct cgacaaaagg aagcgagcga ggcgtcggtt
     3721 tagcacttgt caaacaacag gtagaaaatc tcggcggcag catcgccgtg gaatcggaac
     3781 ccgggatttt cacacaattt tttgtccaga taccctggga cggggagagg tcgaacagag
     3841 ctagtgctag cggatccacc ggtgtctaga aagctttgag gccggcctgc agggatcagc
     3901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4021 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4081 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4141 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4501 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4561 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4621 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4681 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4741 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4801 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4861 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4921 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4981 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5161 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5221 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5281 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5341 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5401 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5461 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5521 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5581 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5641 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5701 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5761 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5821 ggtt
//
