LOCUS       .                       7547 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..517
                     /label="5' LTR"
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1020..1427
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1437..1807
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     CDS             1909..1938
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3319..3372
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3373..3858
                     /label="Thy1.1"
                     /note="mouse GPI-anchored cell surface glycoprotein of the
                     immunoglobulin superfamily"
     LTR             3932..4446
                     /label="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4618..4634)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4642..4672)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4687..4708)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4996..5584)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5758..6615)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6616..6720)
                     /label="AmpR promoter"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
      781 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
      841 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
      901 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
      961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg
     1021 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
     1081 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
     1141 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
     1201 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
     1261 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
     1321 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
     1381 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
     1441 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
     1501 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
     1561 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
     1621 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
     1681 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
     1741 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
     1801 cctctagccc tcgagaagct tgccaccatg gcctcaccgt tgacccgctt tctgtcgctg
     1861 aacctgctgc tgctgggtga gtcgattatc ctggggagtg gagaagctga gcagaaactt
     1921 atttcagagg aagacctgga ggttcaactc gtcgaatcag gaggtgggct cgttcagcca
     1981 ggaagaagta tgaagctctc atgtgccgtg agcggcttca ccttttcaga ttacgggatg
     2041 gcttgggttc tgcaagcacc taccaagggt ttggagtggg tcgcttctat tagctatgac
     2101 ggtggtcgga catactacag ggatagtgtg aaaggaagat ttaccatctc tcgggataac
     2161 gccaaaaaca cactctatct gcaaatggag tctttgaggt ctgaagatac tgccacttat
     2221 tattgcgcta aggtgctcat acctcactat tttgattact ggggtcaggg agtgatggtc
     2281 accgtgtcct ctggcggagg cggaagtgga ggcggaggat ctggcggcgg aggctctgat
     2341 atacagatga cacaaagtcc agctagtctc tctgcttctc tgggtgagac tgtcaccatt
     2401 gaatgccgcg cctcagagga tatttatagt aatctcgcct ggtaccagca gaagcctggc
     2461 aactcacccc aactgttgat atatgatgcc aacaatcttg ctgatggggt tccttcacgg
     2521 ttttccggat caggatccgg aacccaatat tccttgaaaa tcaactcatt gcaaagcgag
     2581 gatgtggcat catacttttg ccaacaatat aataattatc cacccacttt tggggctggt
     2641 accaaacttg aattgaaaat cgagttcatg tacccccctc cctacctgga caacgagaga
     2701 agcaacggca ccatcatcca catcaaagaa aagcacctgt gccacaccca gagcagcccc
     2761 aagctgttct gggccctggt ggtggtggcc ggcgtgctgt tctgttacgg cctgctggtc
     2821 acagtggccc tgtgcgtgat ctggaccaac agcagaagaa acagaggcgg ccagagcgac
     2881 tacatgaaca tgacccccag aaggccaggc ctgaccagaa agccctacca gccctacgcc
     2941 cctgccagag acttcgccgc ctacagaccc agagccaagt tcagcagatc cgccgagaca
     3001 gccgccaacc tgcaggatcc caaccagctg tacaacgagc tgaacctggg cagacgggag
     3061 gaatacgacg tgctggaaaa gaagagagcc agggaccccg agatgggcgg caagcagcag
     3121 agaagaagaa accctcagga aggcgtctac aacgccctgc agaaagacaa gatggccgag
     3181 gcctacagcg agatcggcac caagggcgag agaagaaggg gcaagggcca cgatggcctg
     3241 taccagggcc tgtccaccgc caccaaggac acctacgacg ccctgcacat gcagaccctg
     3301 gcccccagag gcagtggaga gggcagagga agtcttctaa catgcggtga cgtggaggag
     3361 aatcccggcc ctatgaaccc agccatcagc gtcgctctcc tgctctcagt cttgcaggtg
     3421 tcccgagggc agaaggtgac cagcctgaca gcctgcctgg tgaaccaaaa ccttcgcctg
     3481 gactgccgcc atgagaataa caccaaggat aactccatcc agcatgagtt cagcctgacc
     3541 cgagagaaga ggaagcacgt gctctcaggc acccttggga tacccgagca cacgtaccgc
     3601 tcccgcgtca ccctctccaa ccagccctat atcaaggtcc ttaccctagc caacttcacc
     3661 accaaggatg agggcgacta cttttgtgag cttcgcgtct cgggcgcgaa tcccatgagc
     3721 tccaataaaa gtatcagtgt gtatagagac aagctggtca agtgtggcgg cataagcctg
     3781 ctggttcaga acacatcctg gatgctgctg ctgctgcttt ccctctccct cctccaagcc
     3841 ctggacttca tttctctgtg agtcgacggt accgcgggcc cgggatccga taaaataaaa
     3901 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     3961 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     4021 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     4081 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
     4141 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     4201 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     4261 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
     4321 actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     4381 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     4441 tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata
     4501 ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa
     4561 atccatcgat ggagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6721 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6781 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6841 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6901 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6961 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     7021 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     7081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7141 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7201 gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     7261 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     7321 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     7381 ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca
     7441 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
     7501 ccatagataa aataaaagat tttatttagt ctccagaaaa agggggg
//
