LOCUS       62056_17320        8116 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             727..1243
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     misc_feature    1307..1648
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             1715..2131
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    2164..2740
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2741..4390
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             4460..5167
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     LTR             5237..5751
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(5913..5929)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5937..5967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5982..6003)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6291..6879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7053..7910)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7911..8015)
                     /label=AmpR promoter
ORIGIN
        1 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
       61 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      121 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
      181 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
      241 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      301 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      361 agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa
      421 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
      481 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
      541 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt
      601 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
      661 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
      721 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
      781 caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag
      841 agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      901 ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc
      961 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     1021 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     1081 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat
     1141 tcccaataaa gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg
     1201 tctcctcaga ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga
     1261 tttggagacc cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg
     1321 tcgtttcgtg tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct
     1381 gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1441 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     1501 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     1561 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     1621 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     1681 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
     1741 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1801 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1861 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1921 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1981 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     2041 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     2101 ttatccagcc ctcactcctt ctctaggcgc cggaattaga tctctcgagg ttaacgaatt
     2161 ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     2221 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     2281 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2341 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2401 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2461 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     2521 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     2581 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     2641 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     2701 gttttccttt gaaaaacacg atgataatat ggccacaacc atggaagacg ccaaaaacat
     2761 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
     2821 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
     2881 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
     2941 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
     3001 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
     3061 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
     3121 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
     3181 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
     3241 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
     3301 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
     3361 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
     3421 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
     3481 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     3541 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     3601 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     3661 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     3721 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     3781 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     3841 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     3901 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     3961 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     4021 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
     4081 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
     4141 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     4201 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
     4261 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     4321 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     4381 gatcgccgtg tattctagga tcgctactaa cttcagcctg ctgaagcagg ctggagacgt
     4441 ggaggagaac cctggaccta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     4501 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     4561 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4621 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     4681 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     4741 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     4801 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     4861 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     4921 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     4981 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     5041 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     5101 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     5161 gtacaagtaa gtcgacctgc agccaagctt atcgataaaa taaaagattt tatttagtct
     5221 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     5281 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     5341 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5401 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     5461 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     5521 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     5581 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     5641 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5701 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     5761 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     5821 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     5881 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     5941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6061 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7021 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7081 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7141 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7201 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7261 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7321 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7381 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7441 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7501 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7561 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7621 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7681 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7741 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7801 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7861 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7921 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7981 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8041 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8101 ccctttcgtc tcgcgc
//
