LOCUS       V014300                 5957 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014300
VERSION     V014300
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             750..776
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             777..788
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1179..1832
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             1833..1853
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          1912..2484
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2488..2610
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2681..2736
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(3091..3109)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3116..3132)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3274..3729
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3755..3859
                     /label="AmpR promoter"
     misc_feature    4044..4423
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      4892..5480
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5768..5789
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5804..5834
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5842..5858
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5866..5882
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5903..5921
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctagagtcg acctgcagcc caagcttggg ctgaacatcg agcgccaggg ctccgtaaag
       61 ctactagagc acaggcggtg ccccaacgtc ctggggcctc tccactaata acggctactt
      121 ccaattgatt ggacgcgcca tcttgcctgc cttatgcata ttcagcggtg aactgaatat
      181 tcatgaacga ggcccgtccc gtccctccct cccttccccc ccccccggaa cccgctccgg
      241 aggacccgaa gggccccgcc ttcattaccg atgcgtagga caaaccattt tcccgatgtg
      301 tgtgggggga tactaatgag agactttagc tgaaaatgag cctgaactcc gaagctgagt
      361 aaaaatggcc taactttatc ctccgttctg taagtcctcg gtttgagtgc acgggaaacc
      421 cgaaaggagg acgacaggac caggaacatt ctcctcctcc tgtcgcgtca gaaagaacac
      481 ccaaccaggg agccggagcc ctagcgtcaa caactccgcc gcgcgcgctc cgtgtaggcc
      541 ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg acacaagggc
      601 gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag agcgcgaccg
      661 ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc gtaacctctg
      721 ccgttcagga tcactagtcc accatgggct acccctacga cgtgcccgac tacgccatcg
      781 aaggccgcca tatgctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg
      841 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
      901 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
      961 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
     1021 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
     1081 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
     1141 caaactccgg catctacgcg gatccaccgg tcgccaccat gtcccctata ctaggttatt
     1201 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
     1261 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
     1321 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
     1381 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
     1441 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
     1501 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
     1561 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
     1621 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
     1681 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     1741 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     1801 ccacgtttgg tggtggcgac catcctccaa aaccaaaaaa gaagagaaag gtataaatcg
     1861 attctgcagt agctcgccgg cagctgacta gctagagctg agaacttcag ggtgagtttg
     1921 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     1981 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca
     2041 tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc
     2101 ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa
     2161 ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc
     2221 aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa
     2281 tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt
     2341 agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca atgatataca
     2401 ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt
     2461 catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat
     2521 cattttggca aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     2581 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     2641 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     2701 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2761 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     2821 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     2881 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     2941 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     3001 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     3061 tccctcgagc ggccgcggta cccaattcgc cctatagtga gtcgtattac aattcactgg
     3121 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3181 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3241 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3301 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3361 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3421 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3481 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3541 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3601 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3661 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3721 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3841 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3901 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3961 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4021 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4081 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4141 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4201 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4261 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaaacatgg
     4321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5641 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5701 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5761 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5821 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5881 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc
     5941 accgcggtgg cggccgc
//
