LOCUS       62056_17245        3540 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(141..998)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(999..1103)
                     /label=AmpR promoter
     primer_bind     1577..1593
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1669..1700
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             1733..2449
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2517..2533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2541..2557)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2565..2595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2610..2631)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2919..3507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
       61 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
      781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1141 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     1201 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     1261 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     1321 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     1381 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     1441 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1501 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1561 tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac ctgagctcgc
     1621 tagcctcgag gatatcaaga tctggcctcg gcggccaagc ttagacacta gagggtatat
     1681 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggtgag
     1741 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1801 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1861 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1921 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1981 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2041 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2101 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2161 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2221 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2281 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2341 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2401 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aatctagata
     2461 tcggatcccg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca
     2521 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2581 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2641 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2701 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2761 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2821 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2881 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2941 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3001 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3061 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3481 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
//
