LOCUS       V004681                10411 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004681
VERSION     V004681
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10411)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    156..180
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      481..1080
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           1081..1718
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
     CDS             1721..1948
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label="EPSPS CTP"
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17828.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             1949..3313
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      3323..3575
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3897..4241
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4264..4938
                     /gene="BBX32"
                     /label="B-box zinc finger protein 32"
                     /note="B-box zinc finger protein 32 from Arabidopsis
                     thaliana. Accession#: Q9LJB7"
     3'UTR           4956..5270
                     /label="derived from G. barbadense E6"
                     /note="derived from G. barbadense E6"
     misc_feature    5341..5365
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5794..6682
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             complement(5852..6640)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7213..7801
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(7987..8127)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(8232..8420)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      9709..10323
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      9800..10331
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 aaaagtccca tgtggatcac tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca
       61 catggctcag ttctcaatgg aaattatctg cctaaccggc tcagttctgc gtagaaacca
      121 acatgcaagc tccaccgggt gcaaagcggc agcggcggca ggatatattc aattgtaaat
      181 ggcttcatgt ccgggaaatc tacatggatc agcaatgagt atgatggtca atatggagaa
      241 aaagaaagag taattaccaa ttttttttca attcaaaaat gtagatgtcc gcagcgttat
      301 tataaaatga aagtacattt tgataaaacg acaaattacg atccgtcgta tttataggcg
      361 aaagcaataa acaaattatt ctaattcgga aatctttatt tcgacgtgtc tacattcacg
      421 tccaaatggg ggcttagatg agaaacttca cgatcgatgc ggccaccact cgagaagctt
      481 actagtcaac aattggccaa tctttgttct aaattgctaa taaacgacca tttccgtcaa
      541 ttctccttgg ttgcaacagt ctacccgtca aatgtttact aatttataag tgtgaagttt
      601 gaattatgaa agacgaaatc gtattaaaaa ttcacaagaa taaacaactc catagatttt
      661 caaaaaaaca gtcacgagaa aaaaaccaca gtccgtttgt ctgctcttct agtttttatt
      721 atttttctat taatagtttt ttgttatttc gagaataaaa tttgaacgat gtccgaacca
      781 caaaagccga gccgataaat cctaagccga gcctaacttt agccgtaacc atcagtcacg
      841 gctcccgggc taattcattt gaaccgaatc ataatcaacg gtttagatca aactcaaaac
      901 aatctaacgg caacatagac gcgtcggtga gctaaaaaga gtgtgaaagc caggtcacca
      961 tagcattgtc tctcccagat tttttatttg ggaaataata gaagaaatag aaaaaaataa
     1021 aagagtgaga aaaatcgtag agctatatat tcgcacatgt actcgtttcg ctttccttag
     1081 tgttagctgc tgccgctgtt gtttctcctc catttctcta tctttctctc tcgctgcttc
     1141 tcgaatcttc tgtatcatct tcttcttctt caaggtgagt ctctagatcc gttcgcttga
     1201 ttttgctgct cgttagtcgt tattgttgat tctctatgcc gatttcgcta gatctgttta
     1261 gcatgcgttg tggttttatg agaaaatctt tgttttgggg gttgcttgtt atgtgattcg
     1321 atccgtgctt gttggatcga tctgagctaa ttcttaaggt ttatgtgtta gatctatgga
     1381 gtttgaggat tcttctcgct tctgtcgatc tctcgctgtt atttttgttt ttttcagtga
     1441 agtgaagttg tttagttcga aatgacttcg tgtatgctcg attgatctgg ttttaatctt
     1501 cgatctgtta ggtgttgatg tttacaagtg aattctagtg ttttctcgtt gagatctgtg
     1561 aagtttgaac ctagttttct caataatcaa catatgaagc gatgtttgag tttcaataaa
     1621 cgctgctaat cttcgaaact aagttgtgat ctgattcgtg tttacttcat gagcttatcc
     1681 aattcatttc ggtttcattt tacttttttt ttagtgaacc atggcgcaag ttagcagaat
     1741 ctgcaatggt gtgcagaacc catctcttat ctccaatctc tcgaaatcca gtcaacgcaa
     1801 atctccctta tcggtttctc tgaagacgca gcagcatcca cgagcttatc cgatttcgtc
     1861 gtcgtgggga ttgaagaaga gtgggatgac gttaattggc tctgagcttc gtcctcttaa
     1921 ggtcatgtct tctgtttcca cggcgtgcat gcttcatgga gcttcatcta ggccagctac
     1981 tgccaggaag tctagcgggc tcagtggcac cgtgcgcatc cctggcgata aaagtatttc
     2041 acacaggagc ttcatgttcg gaggacttgc tagtggagag acgagaatca ctggtttgct
     2101 tgagggcgaa gatgttatca acaccggtaa ggcgatgcaa gcaatgggtg ccagaatccg
     2161 aaaagagggc gatacgtgga tcatcgacgg tgttggtaac ggaggattgc tcgctcccga
     2221 agcgccactt gactttggga acgcagctac ggggtgccgt cttactatgg gactggtagg
     2281 cgtgtatgac tttgactcta ccttcatcgg tgacgcgagc ctcactaaga gaccaatggg
     2341 acgagtgctg aatcccctga gggagatggg tgtccaggtg aaatctgagg atggtgatcg
     2401 tcttccggtt actctgcgag gccccaagac ccccacgcca atcacgtaca gggttccgat
     2461 ggcgtcagca caggtcaagt cagcggtact cctggcgggc ctcaacacac ctggaatcac
     2521 aaccgtgatt gaacccatca tgactagaga ccacacggag aagatgttgc agggtttcgg
     2581 cgctaatcta acggtcgaaa ccgacgccga cggcgtgagg acaatccgct tggagggcag
     2641 aggtaaactg actggccaag tcatcgatgt gcctggagat ccctcgtcca cagcgtttcc
     2701 cctcgtagct gcgttgctcg tccctggatc tgatgtgacg atcctgaatg tcctcatgaa
     2761 tccaactaga accggcctca tcctcacatt gcaggagatg ggtgctgaca tcgaggttat
     2821 caatcctagg ttggcaggtg gagaggatgt ggccgatctg cgcgtgcgtt ctagtacact
     2881 caaaggcgtg accgtccctg aggatcgcgc tccatccatg atcgacgagt accccattct
     2941 cgccgttgct gctgcgtttg ccgagggcgc aactgtaatg aacggccttg aggagttgag
     3001 ggttaaggag agtgacaggc tgtccgcggt ggcgaatggc ctgaagctaa acggcgtgga
     3061 ctgcgacgaa ggtgaaacgt cccttgtagt ccgtggtcgc ccagacggga aggggttggg
     3121 gaatgcttcg ggagctgctg tggcgacgca ccttgatcat agaatcgcca tgtcatttct
     3181 ggtgatggga cttgtctccg agaatccggt gaccgttgac gatgctacca tgatcgccac
     3241 ctcctttcct gagttcatgg acctcatggc aggcttgggg gccaagatcg agctgtctga
     3301 tactaaggcc gcttgaattc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3361 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3421 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3481 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3541 attatcgcgc gcggtgtcat ctatgttact agatcgggga tcccacgtgc ggaccgcctg
     3601 caggccgcgt tatcaagcta actgcaggtc cgatgtgaga cttttcaaca aagggtaata
     3661 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     3721 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     3781 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     3841 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tccgattgag
     3901 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     3961 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     4021 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     4081 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     4141 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     4201 ccttcctcta tataaggaag ttcatttcat ttggagagga ccaggtggta ccggcgcgcc
     4261 accatggtga gcttttgcga gctttgtggt gccgaagctg atctccattg tgccgcggac
     4321 tctgccttcc tctgccgttc ttgtgacgct aagttccatg cctcaaattt tctcttcgct
     4381 cgtcatttcc ggcgtgtcat ctgcccaaat tgcaaatctc ttactcaaaa tttcgtttct
     4441 ggtcctcttc ttccttggcc tccacgaaca acatgttgtt cagaatcgtc gtcttcttct
     4501 tgctgctcgt ctcttgactg tgtctcaagc tccgagctat cgtcaacgac gcgtgacgta
     4561 aacagagcgc gagggaggga aaacagagtg aatgccaagg ccgttgcggt tacggtggcg
     4621 gatggcattt ttgtaaattg gtgtggtaag ttaggactaa acagggattt aacaaacgct
     4681 gtcgtttcat atgcgtcttt ggctttggct gtggagacga ggccaagagc gacgaagaga
     4741 gtgttcttag cggcggcgtt ttggttcggc gttaagaaca cgacgacgtg gcagaattta
     4801 aagaaagtag aagatgtgac tggagtttca gctgggatga ttcgagcggt tgaaagcaaa
     4861 ttggcgcgtg caatgacgca gcagcttaga cggtggcgcg tggattcgga ggaaggatgg
     4921 gctgaaaacg acaacgttta ggcgatcgcg ggccctgatc acctgtcgta cagtatttct
     4981 acatttgatg tgtgatttgt gaagaacatc aaacaaaaca agcactggct ttaatatgat
     5041 gataagtatt atggtaatta attaattggc aaaaacaaca atgaagctaa aattttattt
     5101 attgagcctt gcggttaatt tcttgtgatg atcttttttt ttattttcta attatatata
     5161 gtttcctttg ctttgaaatg ctaaaggttt gagagagtta tgctcttttt ttcttcctct
     5221 ttctttttta actttatcat acaaattttg aataaaaatg tgagtacatt gagctcattt
     5281 aaataagctt gatggggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     5341 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     5401 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     5461 gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa
     5521 acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc
     5581 ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg
     5641 cagcgccgaa aaatcctcga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5701 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5761 cgacgagcgt gacaccacga tgcctgcagc atctaacgct tgagttaagc cgcgccgcga
     5821 agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     5881 ctttcacgta gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     5941 gtccaagata agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     6001 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     6061 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
     6121 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     6181 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     6241 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     6301 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     6361 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     6421 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     6481 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     6541 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     6601 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta
     6661 agccgcgccg cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg
     6721 acctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaacgc tttttccgaa
     7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7981 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     8041 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     8101 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     8161 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8221 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     8281 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     8341 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     8401 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     8461 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     8521 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     8581 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     8641 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     8701 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     8761 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     8821 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     8881 acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg
     8941 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     9001 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc
     9061 aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata
     9121 gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg
     9181 ccgtgacgat cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt
     9241 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     9301 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     9361 cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct
     9421 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     9481 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     9541 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     9601 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     9661 aggctctcaa gggcatcggt cgatcgaccc tttccgacgc tcaccgggct ggttgccctc
     9721 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg
     9781 tgtgcgagac accgcggccg gccgccggcg ttgtggatac ctcgcggaaa acttggccct
     9841 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
     9901 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
     9961 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
    10021 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
    10081 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
    10141 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
    10201 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
    10261 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
    10321 cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg
    10381 gcctcacccc aaaaatggca gccaagctag g
//
