LOCUS       V004682                 9754 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004682
VERSION     V004682
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9754)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9754)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    51..75
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(504..1392)
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             complement(562..1350)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1923..2511
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2697..2837)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2942..3130)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      4398..5111
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    5277..5301
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      5602..6201
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           6202..6839
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
     CDS             6842..7069
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label="EPSPS CTP"
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17833.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             7070..8434
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      8444..8696
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9018..9362
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     3'UTR           9420..9734
                     /label="derived from G. barbadense E6"
                     /note="derived from G. barbadense E6"
ORIGIN
        1 gatggggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
       61 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
      121 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
      181 cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa acgtcggaaa
      241 gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc ggctagaagg
      301 tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa
      361 aaatcctcga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      421 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      481 gacaccacga tgcctgcagc atctaacgct tgagttaagc cgcgccgcga agcggcgtcg
      541 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
      601 gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata
      661 agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      721 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      781 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      841 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      901 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      961 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     1021 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     1081 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     1141 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     1201 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     1261 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     1321 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg
     1381 cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg acctgcagca
     1441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2041 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     2701 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     2761 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     2821 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2881 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     2941 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     3001 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     3061 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     3121 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     3181 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     3241 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     3301 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     3361 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     3421 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     3481 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     3541 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     3601 aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     3661 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     3721 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     3781 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     3841 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     3901 cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     3961 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     4021 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     4081 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     4141 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     4201 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     4261 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     4321 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     4381 gggcatcggt cgatcgaccc tttccgacgc tcaccgggct ggttgccctc gccgctgggc
     4441 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac
     4501 accgcggccg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     4561 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     4621 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     4681 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     4741 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     4801 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     4861 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     4921 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     4981 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     5041 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     5101 aaaaatggca gccaagctag gaaaagtccc atgtggatca ctccgttgcc ccgtcgctca
     5161 ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg
     5221 ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc
     5281 aggatatatt caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag
     5341 tatgatggtc aatatggaga aaaagaaaga gtaattacca attttttttc aattcaaaaa
     5401 tgtagatgtc cgcagcgtta ttataaaatg aaagtacatt ttgataaaac gacaaattac
     5461 gatccgtcgt atttataggc gaaagcaata aacaaattat tctaattcgg aaatctttat
     5521 ttcgacgtgt ctacattcac gtccaaatgg gggcttagat gagaaacttc acgatcgatg
     5581 cggccaccac tcgagaagct tactagtcaa caattggcca atctttgttc taaattgcta
     5641 ataaacgacc atttccgtca attctccttg gttgcaacag tctacccgtc aaatgtttac
     5701 taatttataa gtgtgaagtt tgaattatga aagacgaaat cgtattaaaa attcacaaga
     5761 ataaacaact ccatagattt tcaaaaaaac agtcacgaga aaaaaaccac agtccgtttg
     5821 tctgctcttc tagtttttat tatttttcta ttaatagttt tttgttattt cgagaataaa
     5881 atttgaacga tgtccgaacc acaaaagccg agccgataaa tcctaagccg agcctaactt
     5941 tagccgtaac catcagtcac ggctcccggg ctaattcatt tgaaccgaat cataatcaac
     6001 ggtttagatc aaactcaaaa caatctaacg gcaacataga cgcgtcggtg agctaaaaag
     6061 agtgtgaaag ccaggtcacc atagcattgt ctctcccaga ttttttattt gggaaataat
     6121 agaagaaata gaaaaaaata aaagagtgag aaaaatcgta gagctatata ttcgcacatg
     6181 tactcgtttc gctttcctta gtgttagctg ctgccgctgt tgtttctcct ccatttctct
     6241 atctttctct ctcgctgctt ctcgaatctt ctgtatcatc ttcttcttct tcaaggtgag
     6301 tctctagatc cgttcgcttg attttgctgc tcgttagtcg ttattgttga ttctctatgc
     6361 cgatttcgct agatctgttt agcatgcgtt gtggttttat gagaaaatct ttgttttggg
     6421 ggttgcttgt tatgtgattc gatccgtgct tgttggatcg atctgagcta attcttaagg
     6481 tttatgtgtt agatctatgg agtttgagga ttcttctcgc ttctgtcgat ctctcgctgt
     6541 tatttttgtt tttttcagtg aagtgaagtt gtttagttcg aaatgacttc gtgtatgctc
     6601 gattgatctg gttttaatct tcgatctgtt aggtgttgat gtttacaagt gaattctagt
     6661 gttttctcgt tgagatctgt gaagtttgaa cctagttttc tcaataatca acatatgaag
     6721 cgatgtttga gtttcaataa acgctgctaa tcttcgaaac taagttgtga tctgattcgt
     6781 gtttacttca tgagcttatc caattcattt cggtttcatt ttactttttt tttagtgaac
     6841 catggcgcaa gttagcagaa tctgcaatgg tgtgcagaac ccatctctta tctccaatct
     6901 ctcgaaatcc agtcaacgca aatctccctt atcggtttct ctgaagacgc agcagcatcc
     6961 acgagcttat ccgatttcgt cgtcgtgggg attgaagaag agtgggatga cgttaattgg
     7021 ctctgagctt cgtcctctta aggtcatgtc ttctgtttcc acggcgtgca tgcttcatgg
     7081 agcttcatct aggccagcta ctgccaggaa gtctagcggg ctcagtggca ccgtgcgcat
     7141 ccctggcgat aaaagtattt cacacaggag cttcatgttc ggaggacttg ctagtggaga
     7201 gacgagaatc actggtttgc ttgagggcga agatgttatc aacaccggta aggcgatgca
     7261 agcaatgggt gccagaatcc gaaaagaggg cgatacgtgg atcatcgacg gtgttggtaa
     7321 cggaggattg ctcgctcccg aagcgccact tgactttggg aacgcagcta cggggtgccg
     7381 tcttactatg ggactggtag gcgtgtatga ctttgactct accttcatcg gtgacgcgag
     7441 cctcactaag agaccaatgg gacgagtgct gaatcccctg agggagatgg gtgtccaggt
     7501 gaaatctgag gatggtgatc gtcttccggt tactctgcga ggccccaaga cccccacgcc
     7561 aatcacgtac agggttccga tggcgtcagc acaggtcaag tcagcggtac tcctggcggg
     7621 cctcaacaca cctggaatca caaccgtgat tgaacccatc atgactagag accacacgga
     7681 gaagatgttg cagggtttcg gcgctaatct aacggtcgaa accgacgccg acggcgtgag
     7741 gacaatccgc ttggagggca gaggtaaact gactggccaa gtcatcgatg tgcctggaga
     7801 tccctcgtcc acagcgtttc ccctcgtagc tgcgttgctc gtccctggat ctgatgtgac
     7861 gatcctgaat gtcctcatga atccaactag aaccggcctc atcctcacat tgcaggagat
     7921 gggtgctgac atcgaggtta tcaatcctag gttggcaggt ggagaggatg tggccgatct
     7981 gcgcgtgcgt tctagtacac tcaaaggcgt gaccgtccct gaggatcgcg ctccatccat
     8041 gatcgacgag taccccattc tcgccgttgc tgctgcgttt gccgagggcg caactgtaat
     8101 gaacggcctt gaggagttga gggttaagga gagtgacagg ctgtccgcgg tggcgaatgg
     8161 cctgaagcta aacggcgtgg actgcgacga aggtgaaacg tcccttgtag tccgtggtcg
     8221 cccagacggg aaggggttgg ggaatgcttc gggagctgct gtggcgacgc accttgatca
     8281 tagaatcgcc atgtcatttc tggtgatggg acttgtctcc gagaatccgg tgaccgttga
     8341 cgatgctacc atgatcgcca cctcctttcc tgagttcatg gacctcatgg caggcttggg
     8401 ggccaagatc gagctgtctg atactaaggc cgcttgaatt cccgatcgtt caaacatttg
     8461 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     8521 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     8581 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     8641 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggg
     8701 atcccacgtg cggaccgcct gcaggccgcg ttatcaagct aactgcaggt ccgatgtgag
     8761 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     8821 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     8881 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     8941 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     9001 tgatgtgatg gtccgattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     9061 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     9121 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     9181 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     9241 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     9301 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     9361 accaggtggt accggcgcgc caggcctggt agtctgatta attaagcgat cgcgggccct
     9421 gatcacctgt cgtacagtat ttctacattt gatgtgtgat ttgtgaagaa catcaaacaa
     9481 aacaagcact ggctttaata tgatgataag tattatggta attaattaat tggcaaaaac
     9541 aacaatgaag ctaaaatttt atttattgag ccttgcggtt aatttcttgt gatgatcttt
     9601 ttttttattt tctaattata tatagtttcc tttgctttga aatgctaaag gtttgagaga
     9661 gttatgctct ttttttcttc ctctttcttt tttaacttta tcatacaaat tttgaataaa
     9721 aatgtgagta cattgagctc atttaaataa gctt
//
