LOCUS       V004684                 9837 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004684
VERSION     V004684
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9837)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..621
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5'UTR           629..717
                     /label="derived from P. hybrida DnaK"
                     /note="derived from P. hybrida DnaK"
     3'UTR           796..1110
                     /label="derived from G. barbadense E6"
                     /note="derived from G. barbadense E6"
     misc_feature    1181..1205
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(1634..2522)
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             complement(1692..2480)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3053..3641
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3827..3967)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4072..4260)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      5528..6241
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    6407..6431
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      6732..7331
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           7332..7969
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
     CDS             7972..8199
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label="EPSPS CTP"
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17831.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             8200..9564
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      9574..9826
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gggcaggtcc gatgtgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
       61 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
      121 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
      181 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      241 tcttcaaagc aagtggattg atgtgatggt ccgattgaga cttttcaaca aagggtaata
      301 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      361 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      421 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      481 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      541 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      601 tcatttcatt tggagaggac actctagaca gaaaaatttg ctacattgtt tcacaaactt
      661 caaatattat tcatttattt gtcagctttc aaactctttg tttcttgttt gttgattaac
      721 ccttaattaa tccgggcgcg cctcagcagt cgctgtcgtt aacccagcgg tactcgctga
      781 ggcgatcgcg ggccctgatc acctgtcgta cagtatttct acatttgatg tgtgatttgt
      841 gaagaacatc aaacaaaaca agcactggct ttaatatgat gataagtatt atggtaatta
      901 attaattggc aaaaacaaca atgaagctaa aattttattt attgagcctt gcggttaatt
      961 tcttgtgatg atcttttttt ttattttcta attatatata gtttcctttg ctttgaaatg
     1021 ctaaaggttt gagagagtta tgctcttttt ttcttcctct ttctttttta actttatcat
     1081 acaaattttg aataaaaatg tgagtacatt gagctcattt aaataagctt gatggggatc
     1141 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     1201 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     1261 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct
     1321 tggcattccg tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac
     1381 ggagcagcat tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc
     1441 tgctgtggct tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga
     1501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1621 tgcctgcagc atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga
     1681 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtgaacaaat
     1741 tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata agcctgccta
     1801 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     1861 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     1921 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     1981 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     2041 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     2101 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     2161 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     2221 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     2281 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     2341 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     2401 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     2461 gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg
     2521 tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg acctgcagca atggcaacaa
     2581 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2641 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2701 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2761 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2821 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2881 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2941 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3001 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3061 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3181 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3721 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3781 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     3841 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     3901 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     3961 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4021 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4081 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     4141 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     4201 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     4261 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     4321 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     4381 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     4441 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     4501 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     4561 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     4621 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     4681 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac
     4741 gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     4801 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     4861 agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc
     4921 catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat
     4981 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
     5041 atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     5101 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     5161 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     5221 cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     5281 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     5341 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     5401 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     5461 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     5521 cgatcgaccc tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt
     5581 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
     5641 gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg
     5701 acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc
     5761 gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt
     5821 acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac
     5881 acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga
     5941 gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat
     6001 ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg
     6061 cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac
     6121 cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc
     6181 ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca
     6241 gccaagctag gaaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg
     6301 gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg
     6361 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc aggatatatt
     6421 caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag tatgatggtc
     6481 aatatggaga aaaagaaaga gtaattacca attttttttc aattcaaaaa tgtagatgtc
     6541 cgcagcgtta ttataaaatg aaagtacatt ttgataaaac gacaaattac gatccgtcgt
     6601 atttataggc gaaagcaata aacaaattat tctaattcgg aaatctttat ttcgacgtgt
     6661 ctacattcac gtccaaatgg gggcttagat gagaaacttc acgatcgatg cggccaccac
     6721 tcgagaagct tactagtcaa caattggcca atctttgttc taaattgcta ataaacgacc
     6781 atttccgtca attctccttg gttgcaacag tctacccgtc aaatgtttac taatttataa
     6841 gtgtgaagtt tgaattatga aagacgaaat cgtattaaaa attcacaaga ataaacaact
     6901 ccatagattt tcaaaaaaac agtcacgaga aaaaaaccac agtccgtttg tctgctcttc
     6961 tagtttttat tatttttcta ttaatagttt tttgttattt cgagaataaa atttgaacga
     7021 tgtccgaacc acaaaagccg agccgataaa tcctaagccg agcctaactt tagccgtaac
     7081 catcagtcac ggctcccggg ctaattcatt tgaaccgaat cataatcaac ggtttagatc
     7141 aaactcaaaa caatctaacg gcaacataga cgcgtcggtg agctaaaaag agtgtgaaag
     7201 ccaggtcacc atagcattgt ctctcccaga ttttttattt gggaaataat agaagaaata
     7261 gaaaaaaata aaagagtgag aaaaatcgta gagctatata ttcgcacatg tactcgtttc
     7321 gctttcctta gtgttagctg ctgccgctgt tgtttctcct ccatttctct atctttctct
     7381 ctcgctgctt ctcgaatctt ctgtatcatc ttcttcttct tcaaggtgag tctctagatc
     7441 cgttcgcttg attttgctgc tcgttagtcg ttattgttga ttctctatgc cgatttcgct
     7501 agatctgttt agcatgcgtt gtggttttat gagaaaatct ttgttttggg ggttgcttgt
     7561 tatgtgattc gatccgtgct tgttggatcg atctgagcta attcttaagg tttatgtgtt
     7621 agatctatgg agtttgagga ttcttctcgc ttctgtcgat ctctcgctgt tatttttgtt
     7681 tttttcagtg aagtgaagtt gtttagttcg aaatgacttc gtgtatgctc gattgatctg
     7741 gttttaatct tcgatctgtt aggtgttgat gtttacaagt gaattctagt gttttctcgt
     7801 tgagatctgt gaagtttgaa cctagttttc tcaataatca acatatgaag cgatgtttga
     7861 gtttcaataa acgctgctaa tcttcgaaac taagttgtga tctgattcgt gtttacttca
     7921 tgagcttatc caattcattt cggtttcatt ttactttttt tttagtgaac catggcgcaa
     7981 gttagcagaa tctgcaatgg tgtgcagaac ccatctctta tctccaatct ctcgaaatcc
     8041 agtcaacgca aatctccctt atcggtttct ctgaagacgc agcagcatcc acgagcttat
     8101 ccgatttcgt cgtcgtgggg attgaagaag agtgggatga cgttaattgg ctctgagctt
     8161 cgtcctctta aggtcatgtc ttctgtttcc acggcgtgca tgcttcatgg agcttcatct
     8221 aggccagcta ctgccaggaa gtctagcggg ctcagtggca ccgtgcgcat ccctggcgat
     8281 aaaagtattt cacacaggag cttcatgttc ggaggacttg ctagtggaga gacgagaatc
     8341 actggtttgc ttgagggcga agatgttatc aacaccggta aggcgatgca agcaatgggt
     8401 gccagaatcc gaaaagaggg cgatacgtgg atcatcgacg gtgttggtaa cggaggattg
     8461 ctcgctcccg aagcgccact tgactttggg aacgcagcta cggggtgccg tcttactatg
     8521 ggactggtag gcgtgtatga ctttgactct accttcatcg gtgacgcgag cctcactaag
     8581 agaccaatgg gacgagtgct gaatcccctg agggagatgg gtgtccaggt gaaatctgag
     8641 gatggtgatc gtcttccggt tactctgcga ggccccaaga cccccacgcc aatcacgtac
     8701 agggttccga tggcgtcagc acaggtcaag tcagcggtac tcctggcggg cctcaacaca
     8761 cctggaatca caaccgtgat tgaacccatc atgactagag accacacgga gaagatgttg
     8821 cagggtttcg gcgctaatct aacggtcgaa accgacgccg acggcgtgag gacaatccgc
     8881 ttggagggca gaggtaaact gactggccaa gtcatcgatg tgcctggaga tccctcgtcc
     8941 acagcgtttc ccctcgtagc tgcgttgctc gtccctggat ctgatgtgac gatcctgaat
     9001 gtcctcatga atccaactag aaccggcctc atcctcacat tgcaggagat gggtgctgac
     9061 atcgaggtta tcaatcctag gttggcaggt ggagaggatg tggccgatct gcgcgtgcgt
     9121 tctagtacac tcaaaggcgt gaccgtccct gaggatcgcg ctccatccat gatcgacgag
     9181 taccccattc tcgccgttgc tgctgcgttt gccgagggcg caactgtaat gaacggcctt
     9241 gaggagttga gggttaagga gagtgacagg ctgtccgcgg tggcgaatgg cctgaagcta
     9301 aacggcgtgg actgcgacga aggtgaaacg tcccttgtag tccgtggtcg cccagacggg
     9361 aaggggttgg ggaatgcttc gggagctgct gtggcgacgc accttgatca tagaatcgcc
     9421 atgtcatttc tggtgatggg acttgtctcc gagaatccgg tgaccgttga cgatgctacc
     9481 atgatcgcca cctcctttcc tgagttcatg gacctcatgg caggcttggg ggccaagatc
     9541 gagctgtctg atactaaggc cgcttgaatt cccgatcgtt caaacatttg gcaataaagt
     9601 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     9661 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     9721 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     9781 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggg atcccac
//
