LOCUS       62056_16965        5150 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    2161..2216
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2577..2593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2601..2617)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2625..2655)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2670..2691)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2749..2945
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2951..3085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3324..3912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4086..4943)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4944..5048)
                     /label=AmpR promoter
     enhancer        5079..5150
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atcgccacca tgaagcggaa gcgcgagcag atgacactgt ggaaggccgc
      601 cttcgtgaac ggccaagaga cattcaagag ctggatcgac aaggcccgga tgctggaact
      661 gaactgcgac gtgtccagcg ccagcagcac acactacagc gacctgaacc tgaaaacaaa
      721 gtgcgccaag accgacgaca agttcatgtg caactacagc gtgtgcatca gacccaccag
      781 caagcagaag cggaccctga accagatgct gaaggtgtcc aactacgcct acaactggtg
      841 caattacctg gtcaaagaga aggacttcaa gcccaagcag ttcgacctgc agcggatcgt
      901 ggccaagacc aacagcacag atgtgcccgc cgagtacaga ctgcctggcg acgattggtt
      961 cttcgacaac aagatgagca gcatcaagct gaccgcctgc aagaacttct gcaccatgta
     1021 caagagcacc cagaccaacc agaaaaagac caaggtggac ctgcggaaca aggacatcgt
     1081 gcagctgaga gaaggcagct tcgaggtgca gagcaaatac gtgcggctgc tgaccgagaa
     1141 ggacatccct ggcgagagaa tccggcagag cagaatcgcc ctgatgcctg acagcttcag
     1201 caagagcaag aaggactgga aagagcggtt cctgcggctg agcaagaacg tgtccaagat
     1261 tcctccactg agccacgaca tgaaagtgtg caagcggccc aacggcaagt tcatcctgca
     1321 gatcagctgc gaccccatct gcaccagaca gatccaggtg cagaccagcg acagcatctg
     1381 cagcattgat cctggcggca gaaccttcgc cacctgttac gaccccagca acatcaagac
     1441 cttccagatc ggccctgagg ccgacaagaa agagatcatc cacgagttcc acaacaaaat
     1501 cgactacgtg caccggctgc tgtctcacgc ccaagagaag aaacagaccc aggccgtgca
     1561 ggacagaatc ggccagctga agaagctgca tctgaagctc aagacctacg tggacgacgt
     1621 gcacctgaaa ctgtgcagct acctcgtgaa gaactacaag ctggtggtgc tgggcaagat
     1681 cagcgtgtcc tctatcgtgc ggaaggacag acccaaccac ctggccaaga gcgccaacag
     1741 agatctgctg tgctggcagc actaccggtt cagacagagg ctgctgcata gagtgcgggg
     1801 caccgattgc gaagtgatca tccaggacga gcggtacacc tccaagacct gcggcaattg
     1861 cggcgagaag aacaacaaac tcggcggcaa agaaaccttt acctgcgaga gctgcaatta
     1921 caagacccac cgggatgtga acggcgccag aaacatcctg tgcaagtatc tgggactgtt
     1981 ccccttcgcc gcctgactag cataacccct tggggcgcac tcctcaggtg caggctgcct
     2041 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     2101 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     2161 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     2221 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     2281 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     2341 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     2401 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     2461 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     2521 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     2581 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2641 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2701 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     2761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2941 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3001 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3061 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     3121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3721 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5041 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtaa
     5101 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
