LOCUS       40924_30925        5407 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMJ016c, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 5407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      552..1140
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1428..1449
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1464..1494
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1502..1518
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1526..1542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3857..3873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4086..4541
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4823..4927
                     /label=AmpR promoter
     CDS             join(4928..5407,1..378)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
       61 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct
      241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
      541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      661 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1201 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1261 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1321 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1381 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1441 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1501 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     1561 cttcctcttc cagcccttcc tctttcctcg aggagtcccc gggttggatg cactagtcac
     1621 acgagccctc gtcagaaaca cgtctccgcc acgctctccc tctggtctcc atcaggcagc
     1681 tcgctgaggc ttgacatgat tggtgcgtat gtttgtatga agctacagga ctgatttggc
     1741 gggctatgag ggcgggggaa gctctggaag ggccgcgatg gggcgcgcgg cgtccagaag
     1801 gcgccatacg gcccgctggc ggcacccatc cggtataaaa gcccgcgacc ccgaacggtg
     1861 acctccactt tcagcgacaa acgagcactt atacatacgc gactattctg ccgctataca
     1921 taaccactca gctggcttaa gatcccatca ggcttgcatg ccgggcgcgc cagaaggagc
     1981 gcagccaaac caggatgatg tttgatgggg tatttgagca cttgcaaccc ttatccggaa
     2041 gccccctggc ccacaaaggc taggcgccaa tgcaagcagt tcgcatgcag cccctggagc
     2101 ggtgccctcc tgataaaccg gccagggggc ctatgttctt tactttttta caagagaagt
     2161 cactcaacat cttaaaatgg ccaggtaagt cgacgagcaa gcccggcgga tcaggcagcg
     2221 tgcttgcaga tttgacttgc aacgcccgca ttgtgtcgac gaaggctttt ggctcctctg
     2281 tcgctgtctc aagcagcatc taaccctgcg tcgccgtttc catttgcagg atggccactc
     2341 cgccctcccc ggtgctgaag aatttcgaca tatggacgac gccctgagag ccctgcgcgg
     2401 ccgctaccca ggctgcgagt gggttgtggt tgaagatggc gcttccggtg ctggtgttta
     2461 ccgcctgcgt ggtggtggtc gtgaactgtt tgtgaaggtc gctgccctcg gcgctggcgt
     2521 cggtctgctg ggtgaggcgg agcgtctggt ctggctggct gaggttggca tccccgtccc
     2581 gcgcgtggtc gaaggtggtg gtgatgaacg cgttgcttgg ctcgtgaccg aggctgtccc
     2641 tggccgtccc gcctccgctc gctggccgcg tgagcaacgt ctggatgtcg cggtcgctct
     2701 ggctggcctc gcccgctcgc tgcacgctct cgactgggaa cgttgcccct ttgaccgctc
     2761 cctggccgtc acagtgccac aggccgctcg tgctgtcgct gaaggctcgg ttgacctgga
     2821 ggatctggac gaagaacgta aaggctggag cggcgagcgc ctcctggctg aactcgaacg
     2881 cacacgccct gccgatgagg acctggccgt ctgccacggt gacctgtgcc ccgacaacgt
     2941 cctgctcgac ccccgtacct gtgaagtgac tggtctgatc gatgttggcc gcgtgggccg
     3001 cgctgaccgt cattcggacc tggccctggt gctgcgcgaa ctggctcacg aggaggatcc
     3061 ctggtttggt cccgagtgct ccgccgcttt tctgcgcgag tacggccgtg gctgggacgg
     3121 cgctgtttcc gaggagaaac tggccttcta ccgcctgctg gacgaatttt tttaatctag
     3181 atggcagcag ctggaccgcc tgtacaatgg agaagagctt tacttgccgg gatggccgat
     3241 ttcgctgatt gatacgggat cgcagctcgg aggctttcgc gctaggggct aggcgaaggg
     3301 cagtggtgac cagggtcggt gtggggtcgg cccacggtca attagccaca ggaggatcag
     3361 ggggaggtag gcacgtcgac ttggtttgcg accccgcagt tttggcggac gtgctgttgt
     3421 agatgttagc gtgtgcgtga gccagtggcc aacgtgccac acccattgag aagaccaacc
     3481 aacttactgg caatatctgc caatgccata ctgcatgtaa tggccaggcc atgtgagagt
     3541 ttgccgtgcc tgcgcgcgcc ccgggggcgg gtaccgaatc tgctgccggc tgcccccgcc
     3601 cccgctgctg aggctgaatc tactccgctc tccaccccag tcccaggaag agaggcgcat
     3661 ttacttcgca cagacgttac agcacaccct tgatcatcat cagctgctct tccctgccgc
     3721 tgcaacacgc cccgcgctag agacccagag tggcgcccat gactgacgtc gagccttctg
     3781 gcagactagt tgctcctgct tccaacacgt ggactcgata tcgaaagtcc tctccactga
     3841 ctgtagcctc caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3901 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3961 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4021 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4081 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4141 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4201 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4261 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4321 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4381 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4441 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4501 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4561 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4621 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtcaacgg
     4681 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaccc gaaagggcct
     4741 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4801 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5161 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5401 tcgcctt
//
