LOCUS       62056_16885        7110 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1191..2840)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(2865..3068)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3069..3372)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(3494..3512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3524..3542)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3562..3578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3586..3602)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3610..3640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3655..3676)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3964..4552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4726..5583)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5584..5688)
                     /label=AmpR promoter
     polyA_signal    complement(5776..5857)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6090..6686)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(6783..7099)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgta agtgcaaggg taatcattta ttgaacagga agaggaagaa
      541 attcatgaaa aattgtcatg ggtatatcaa agagaaattt aaagaagtct ttgattcttg
      601 atgaggtatc ctaggtaaca tgtggataga ctggaattgg acttctcagt gagacaggat
      661 ggaatgcaga tcacagaatt tatactgggg atgctgtatg gataggaagg gatgatggtg
      721 gagtcctggt gagaagtctc cactcaacac tggaggctca ggccattctt cattctcggg
      781 cctggaggca gcgattggca ttggataggt caatgatatt ttctctaagg attccatgct
      841 cctcacatag ttttgcaaac ctttccttga tgtctgaact caaatctggt tctcggccat
      901 agagccccat cagctggaag gtttccccat ccttttcgtt aatgagatga gccataagaa
      961 agttatcata gtctgtctta ggtatagtaa atgtattgaa tccatcatac gtcacagaat
     1021 attcaccagc cttttctgtt ttgtcagcaa ccatagataa ttcggagcac tcttcatctc
     1081 ttacagtatg gaatttaaga actaaggatt tctccaagac atggatttgc tccagaaaaa
     1141 gtctaaagtt gccattatct tctatctttt ctcttttgtc atcactcgag caatttggac
     1201 tttccgccct tcttggcctt tatgaggatc tctctgattt ttcttgcgtc gagttttccg
     1261 gtaagacctt tcggtacttc gtccacaaac acaactcctc cgcgcaactt tttcgcggtt
     1321 gttacttgac tggcgacgta atccacgatc tctttttccg tcatcgtctt tccgtgctcc
     1381 aaaacaacaa cggcggcggg aagttcaccg gcgtcatcgt cgggaagacc tgccacgccc
     1441 gcgtcgaaga tgttggggtg ttgtaacaat atcgattcca attcagcggg ggccacctga
     1501 tatcctttgt atttaattaa agacttcaag cggtcaacta tgaagaagtg ttcgtcttcg
     1561 tcccagtaag ctatgtctcc agaatgtagc catccatcct tgtcaatcaa ggcgttggtc
     1621 gcttccggat tgtttacata accggacata atcataggtc ctctgacaca taattcgcct
     1681 ctctgattaa cgcccagcgt tttcccggta tccagatcca caaccttcgc ttcaaaaaat
     1741 ggaacaactt taccgaccgc gcccggttta tcatccccct cgggtgtaat cagaatagct
     1801 gatgtagtct cagtgagccc atatccttgt cgtatccctg gaagatggaa gcgttttgca
     1861 accgcttccc cgacttcttt cgaaagaggt gcgcccccag aagcaatttc gtgtaaatta
     1921 gataaatcgt atttgtcaat cagagtgctt ttggcgaaga atgaaaatag ggttggtact
     1981 agcaacgcac tttgaatttt gtaatcctga agggatcgta aaaacagctc ttcttcaaat
     2041 ctatacatta agacgactcg aaatccacat atcaaatatc cgagtgtagt aaacattcca
     2101 aaaccgtgat ggaatgggac aacacttaaa atcgcagtat ccggaacgat ttgattgcca
     2161 aaaataggat ctctggcatg cgagaatctg acgcaggcag ttctatgcgg aagggccaca
     2221 cccttaggta acccagtaga tccagaggaa ttcattatca gtgcaattgt tttgtcacga
     2281 tcaaaggact ctggtacaaa atcgtattca ttaaaaccgg gaggtagatg agatgtgacg
     2341 aacgtgtaca tcgactgaaa tccctggtaa tccgttttag aatccatgat aataattttc
     2401 tggattattg gtaatttttt ttgcacgttc aaaatttttt gcaacccctt tttggaaaca
     2461 aacactacgg taggctgcga aatgttcata ctgttgagca attcacgttc attataaatg
     2521 tcgttcgcgg gcgcaactgc aactccgata aataacgcgc ccaacaccgg cataaagaat
     2581 tgaagagagt tttcactgca tacgacgatt ctgtgatttg tattcagccc atatcgtttc
     2641 atagcttctg ccaaccgaac ggacatttcg aagtattccg cgtacgtgat gttcacctcg
     2701 atatgtgcat ctgtaaaagc aattgttcca ggaaccaggg cgtatctctt catagcctta
     2761 tgcagttgct ctccagcggt tccatcctct agaggataga atggcgccgg gcctttcttt
     2821 atgtttttgg cgtcttccat ggtggatcca cggttcacta aaccagctct gcttatatag
     2881 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     2941 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     3001 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     3061 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     3121 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     3181 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     3241 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     3301 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     3361 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     3421 tattaataac taagatctgg taccttgaat tcatgcttct cctcccttta gtgagggtaa
     3481 ttctctctct ctccctatag tgagtcgtat taattccttc tcttctatag tgtcacctaa
     3541 atcgttgcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4441 taccttcgga aaaagagttg gtagctcttg atccggcaaa aaaaccaccg ctggtagcgg
     4501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5701 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5761 gcgtatcacg agattgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5821 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5881 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     5941 atgtggtatg gctgattatg atcctctaga gtcggtgggc ctcgggggcg ggtgcggggt
     6001 cggcggggcc gccccgggtg gcttcggtcg gagccatggg gtcgtgcgct cctttcggtc
     6061 gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg
     6121 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     6181 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     6241 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     6301 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     6361 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     6421 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     6481 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     6541 agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag
     6601 tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg
     6661 cgcaccgtgg gcttgtactc ggtcatggaa ggtcgtctcc ttgtgagggg tcaggggcgt
     6721 gggtcagggg atggtggcgg caccggtcgt ggcggccgac ctgcaggcat gcaagctagc
     6781 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     6841 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     6901 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     6961 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7021 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     7081 cctggggact ttccacacca atctttcgtc
//
