LOCUS       62056_16875        7794 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1875..3524)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(3549..3752)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3753..4056)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(4178..4196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4208..4226)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4246..4262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4270..4286)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4294..4324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4339..4360)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4648..5236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5410..6267)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6268..6372)
                     /label=AmpR promoter
     polyA_signal    complement(6460..6541)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6774..7370)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(7467..7783)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgta acctcagcaa ctaaaaataa aaacttcagt atataacttg
      541 ggaacaaaaa agtagtaaag ggaagcaatt caagatctgt tattggtatt tgaatacatt
      601 tattgtgaca gaatgctgtt ataaatattc ataagacaaa ggccatcttt ttatctagga
      661 attgtcaaag agaagattcc aaattggaag gatacatctt ttgtaaaatc tgccaccaat
      721 tcctgctttg agaataagca cctattgtaa aatttctaac attataaatg gtcacagcac
      781 atgccacttg atgcaatcca aactttgaaa tgtttgactt ctcagtgggc tgtccctctc
      841 cactgcaacc ccccttcctc cagcctcctg aaacatcgca ctagcctttg gtaagcaatt
      901 ccatatagat acgctggggg agcgtggagt atgaagcctg acatagcatc aggtacatca
      961 ggtacattta tttctaagtc taatagagaa cagtttttac tgcttaatag taagaagcac
     1021 tgagagtgat tttatcgagc attcttaact ctttcaactc cacctagata gactaggatc
     1081 tacagatgat gcagaaaaga ggttagggga gtactttcca atagtttatt gtattttctt
     1141 aaatatcctt tctggaattt tcagaaacaa aacataaaaa aattatatac tttattacaa
     1201 atggtaaact cagagtgctc caaatctctt atttacaaac aacactgggc aggataccca
     1261 aacaaacaaa caagaaataa cttacaaagg catgaagctg tttattgaca gtaatcagct
     1321 ttaatcaaat taaaaaatat atatatgtac atacacagtt aacgaaagca ggccagaaag
     1381 agttcatctg taggctcagc ctcgctctca caaacctccc tcctgccgcc cctccccaac
     1441 cctccccccg ctttgtgttc ttaaggagta ctacagaagc aatctacagt ctctattgca
     1501 gtttgtaacc ccctccccct ccccccttta atactgaatg agatcgaatg ttaggtccat
     1561 gcagttcttg gtcaatgtta acgaaagtcc aacgttccgt tcgcgcgggg acagcccgtc
     1621 cgcaaagcgg ggcagcccgc aggcggccgc tccctggctc cacgccaagg gagggcgcga
     1681 ccaagtcctt cccactcgtg cacactgggg gcgccgtcag accgaaccca gtccaacttg
     1741 gatacccttg gctttagttc tcggacactt cttttttctc tccgtcgcaa cttgtcaagt
     1801 tctcaagtct gtctctctgt gttatttttt ttcttcgttg cccctcagcc cccgacggtc
     1861 tctcttcact cgagcaattt ggactttccg cccttcttgg cctttatgag gatctctctg
     1921 atttttcttg cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact
     1981 cctccgcgca actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt
     2041 tccgtcatcg tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca
     2101 tcgtcgggaa gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgat
     2161 tccaattcag cgggggccac ctgatatcct ttgtatttaa ttaaagactt caagcggtca
     2221 actatgaaga agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca
     2281 tccttgtcaa tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata
     2341 ggtcctctga cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga
     2401 tccacaacct tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc
     2461 ccctcgggtg taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc
     2521 cctggaagat ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc
     2581 ccagaagcaa tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg
     2641 aagaatgaaa atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat
     2701 cgtaaaaaca gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa
     2761 tatccgagtg tagtaaacat tccaaaaccg tgatggaatg ggacaacact taaaatcgca
     2821 gtatccggaa cgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag
     2881 gcagttctat gcggaagggc cacaccctta ggtaacccag tagatccaga ggaattcatt
     2941 atcagtgcaa ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa
     3001 ccgggaggta gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt
     3061 ttagaatcca tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt
     3121 ttttgcaacc cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg
     3181 agcaattcac gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac
     3241 gcgcccaaca ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga
     3301 tttgtattca gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat
     3361 tccgcgtacg tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc
     3421 agggcgtatc tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagagga
     3481 tagaatggcg ccgggccttt ctttatgttt ttggcgtctt ccatggtgga tccacggttc
     3541 actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     3601 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     3661 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     3721 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     3781 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     3841 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     3901 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     3961 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     4021 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     4081 actaatgacc ccgtaattga ttactattaa taactaagat ctggtacctt gaattcatgc
     4141 ttctcctccc tttagtgagg gtaattctct ctctctccct atagtgagtc gtattaattc
     4201 cttctcttct atagtgtcac ctaaatcgtt gcaattcgta atcatgtcat agctgtttcc
     4261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5161 caaaaaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6421 catgacatta acctataaaa ataggcgtat cacgagattg cagtgaaaaa aatgctttat
     6481 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6541 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6601 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagagtcggt
     6661 gggcctcggg ggcgggtgcg gggtcggcgg ggccgccccg ggtggcttcg gtcggagcca
     6721 tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg
     6781 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     6841 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     6901 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     6961 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     7021 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     7081 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     7141 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     7201 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg
     7261 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg
     7321 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggaaggtcgt
     7381 ctccttgtga ggggtcaggg gcgtgggtca ggggatggtg gcggcaccgg tcgtggcggc
     7441 cgacctgcag gcatgcaagc tagctttttg caaaagccta ggcctccaaa aaagcctcct
     7501 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     7561 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     7621 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     7681 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     7741 tgcatacttc tgcctgctgg ggagcctggg gactttccac accaatcttt cgtc
//
