LOCUS       62056_16845        6738 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(819..2468)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(2493..2696)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2697..3000)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(3122..3140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3152..3170)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3190..3206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3214..3230)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3238..3268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3283..3304)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3592..4180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4354..5211)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5212..5316)
                     /label=AmpR promoter
     polyA_signal    complement(5404..5485)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5718..6314)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(6411..6727)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgtt aaagccaagg gcgtggccgg ctctcccaac acagcgactc
      541 ctggaggcca ggtgcccatg ggcctacatc ccctctcagc actgaacagt gagttgattt
      601 ttctttttac aataaaaaaa gctgagtaat attgcatagg agtaccagaa actgcctcat
      661 tggaaacaaa aactatttac attaaataaa aagcctggcc gcaggctgcg tctgccacat
      721 ttacagcacg gtgcgatgca cacggtgacc aaaccacgga ggcagcttct ggcactcaca
      781 ccacgagccg cacgtttgcc acatgagagt cactcgagca atttggactt tccgcccttc
      841 ttggccttta tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc
      901 ggtacttcgt ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg
      961 gcgacgtaat ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg
     1021 gcggcgggaa gttcaccggc gtcatcgtcg ggaagacctg ccacgcccgc gtcgaagatg
     1081 ttggggtgtt gtaacaatat cgattccaat tcagcggggg ccacctgata tcctttgtat
     1141 ttaattaaag acttcaagcg gtcaactatg aagaagtgtt cgtcttcgtc ccagtaagct
     1201 atgtctccag aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg
     1261 tttacataac cggacataat cataggtcct ctgacacata attcgcctct ctgattaacg
     1321 cccagcgttt tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta
     1381 ccgaccgcgc ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca
     1441 gtgagcccat atccttgtcg tatccctgga agatggaagc gttttgcaac cgcttccccg
     1501 acttctttcg aaagaggtgc gcccccagaa gcaatttcgt gtaaattaga taaatcgtat
     1561 ttgtcaatca gagtgctttt ggcgaagaat gaaaataggg ttggtactag caacgcactt
     1621 tgaattttgt aatcctgaag ggatcgtaaa aacagctctt cttcaaatct atacattaag
     1681 acgactcgaa atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg
     1741 aatgggacaa cacttaaaat cgcagtatcc ggaacgattt gattgccaaa aataggatct
     1801 ctggcatgcg agaatctgac gcaggcagtt ctatgcggaa gggccacacc cttaggtaac
     1861 ccagtagatc cagaggaatt cattatcagt gcaattgttt tgtcacgatc aaaggactct
     1921 ggtacaaaat cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc
     1981 gactgaaatc cctggtaatc cgttttagaa tccatgataa taattttctg gattattggt
     2041 aatttttttt gcacgttcaa aattttttgc aacccctttt tggaaacaaa cactacggta
     2101 ggctgcgaaa tgttcatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc
     2161 gcaactgcaa ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt
     2221 tcactgcata cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc
     2281 aaccgaacgg acatttcgaa gtattccgcg tacgtgatgt tcacctcgat atgtgcatct
     2341 gtaaaagcaa ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct
     2401 ccagcggttc catcctctag aggatagaat ggcgccgggc ctttctttat gtttttggcg
     2461 tcttccatgg tggatccacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt
     2521 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     2581 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     2641 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     2701 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     2761 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     2821 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     2881 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     2941 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     3001 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     3061 agatctggta ccttgaattc atgcttctcc tccctttagt gagggtaatt ctctctctct
     3121 ccctatagtg agtcgtatta attccttctc ttctatagtg tcacctaaat cgttgcaatt
     3181 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3241 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3301 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3361 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3421 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3481 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4021 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4081 aagagttggt agctcttgat ccggcaaaaa aaccaccgct ggtagcggtg gtttttttgt
     4141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4441 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4621 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4681 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4981 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5221 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5281 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5341 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5401 attgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5461 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5521 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     5581 tgattatgat cctctagagt cggtgggcct cgggggcggg tgcggggtcg gcggggccgc
     5641 cccgggtggc ttcggtcgga gccatggggt cgtgcgctcc tttcggtcgg gcgctgcggg
     5701 tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     5761 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     5821 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
     5881 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
     5941 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     6001 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     6061 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
     6121 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     6181 cgctcgatgt ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     6241 gcggcgaggg tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc
     6301 ttgtactcgg tcatggaagg tcgtctcctt gtgaggggtc aggggcgtgg gtcaggggat
     6361 ggtggcggca ccggtcgtgg cggccgacct gcaggcatgc aagctagctt tttgcaaaag
     6421 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     6481 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     6541 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     6601 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     6661 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     6721 ccacaccaat ctttcgtc
//
