LOCUS       V004731                13561 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004731
VERSION     V004731
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13561)
  AUTHORS   Lee MH, Nittayajarn A, Ross RP, Rothschild CB, Parsonage D,
            Claiborne A, Rubens CE.
  TITLE     Characterization of Enterococcus faecalis alkaline phosphatase and
            use in identifying Streptococcus agalactiae secreted proteins
  JOURNAL   J. Bacteriol. 181 (18), 5790-5799 (1999)
   PUBMED   10482522
REFERENCE   2  (bases 1 to 13561)
  AUTHORS   Clancy A, Lee MH, Jones AL, Rubens CE.
  TITLE     Construction and characterization of transposon TnphoZ for the
            identification of genes encoding exported proteins in Streptococcus
            agalactiae
  JOURNAL   FEMS Microbiol. Lett. 241 (2), 257-264 (2004)
   PUBMED   15598541
REFERENCE   3  (bases 1 to 13561)
  AUTHORS   Lee MH, Clancy A, Jones AL, Nittayajarn A, Rubens CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2001) Infectious Disease, Children's Hospital and
            Regional Medical Center, 4800 Sand Point Way NE, Seattle, WA 98105,
            USA
REFERENCE   4  (bases 1 to 13561)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 13561)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1999"; volume: "181"; issue: "18"; pages:
            "5790-5799"
            SGRef: number: 2; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2004"; volume: "241"; issue: "2"; pages:
            "257-264"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-MAR-2001) Infectious Disease, Children's Hospital and Regional
            Medical Center, 4800 Sand Point Way NE, Seattle, WA 98105, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             3821..3829
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4170..4904)
                     /gene="ermB"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis (strain ATCC 700802 / V583). Accession#: P20173"
     rep_origin      5356..5944
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6130..6270)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6375..6563)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(7204..8391)
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(8439..8467)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     gene            complement(8683..10032)
                     /gene="phoZ"
                     /allele="phoZ2"
                     /label="phoZ"
                     /note="alkaline phosphatase; derived from Enterococcus
                     faecalis; expression is dependent upon integration event to
                     create in-frame start codon"
     CDS             10619..11410
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(11817..12515)
                     /codon_start=1
                     /gene="repAts"
                     /product="temperature-sensitive RepA"
                     /label="repAts"
                     /protein_id="AAK27832.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            complement(11817..12515)
                     /gene="repAts"
                     /label="repAts"
     CDS             13127..13138
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
ORIGIN
        1 cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg gtttgtagac
       61 aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc taaaaagaac
      121 gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg
      181 agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt
      241 tctttcttat cttgatacat ataggggtcc cgagcgctta gtgggaattt gtatcgataa
      301 ggggtacaaa ttcccactaa accaatgttt caaggcctat ttatttttta tattcaattc
      361 tcttaagtgt ttaggaatag ataacaagtc aaatttatac tctccaagaa aagtgatatg
      421 ctcccaatta ataggagata catgcttcat atacttagtt acttcgggat ctattttgac
      481 gagataatta taagctgctt gtaagtagac ggcgttccat atacttattg catttattaa
      541 cacattaagc gcactagcac tttgaagttg tcggcgaata tcgcgctcca taaattttcc
      601 gcgtcgccca aaaaatagtt ctctacgtaa agcattaatc gcttctgtct tatttagtcc
      661 atgagtgatc cttcgccgta gctcactatc tgtaatataa tctatcataa aaatgctctt
      721 ttcaatgcga cctagttctc tcagtgcaag agctactcta ttcttacgtg cgtatgagcc
      781 tagctttcct aatagtaaag aactagatac ttttcctgtt tgaatcgaat aggcgattcg
      841 tttaatttca tcatagtttt cttcaataat ttttacattg atttttccgc ttatatcttc
      901 tgataagtta gggtagtagg aaggtgattt gatagaaaat aattgtgatt tttttatatt
      961 tctgatgcga ggttcaaaat caaagcctag taatgcggtc attccaaaca cctgatcaga
     1021 atacccattt gtatcagtaa aatgttcctc aatatctaga tctgtttcat gataaagtag
     1081 gccatcaagg gtatgagtag cttcccttgt attagttgaa gcaacctcga tatgatgagt
     1141 cgtatgccta tcatttattg atcgaatcat tgtagctcct ttttccatac ttttgtaatg
     1201 tggattaaca tcggatttta gagctgagac gcccactggg acgcgcattc cgtctgaagc
     1261 agtggttttt ccttcacccc aaaagtctgc aacaggaagc tttaactgat aattaaccaa
     1321 aacagattga gcacgagtca gagcttcttt ataaaagcgc cattgtttgg cattggctaa
     1381 ctgagaataa gaaattccag gagttgattg ggccattttt tcaagaccaa tattcatccc
     1441 taaacccagc aaagcagcaa aaataatttt tctttcttgt tcactcggcg gtttccctgt
     1501 agaatcatga ctaaattctt gtgaaaagtt ggtccaactg tccacctcaa ttaaaagatc
     1561 actaagcctt atcttaggaa ttattgaata aagttttttt ctatatattt ctgcttcgct
     1621 aggtgtaact ttttccaact ttttaaggtt tgctcttgaa attctcttat caactgtcga
     1681 ataaaattga agctgtaaat ctaaaattat ttcccgatcc tttaaatagt cctcaaacgt
     1741 gtctggaata gtttctgaat caatacaagc agataaatca actaagtaat catcaatatt
     1801 tcgatggatc atacttcctt caactgaaat atctcctgat cgaatattgt tctttagctc
     1861 ggtgaaagct actaactcat agtaagaccg atctattttc ccctcttctg gccgaacaag
     1921 gcttttccat tttttactca caaaatcagt agaagtatct gccggtattt ttcttttacc
     1981 actattgtgt aaatcagtta gttgagttag ggccatgagg actggatttg ctgccggagt
     2041 tgctttgaac gaaagggtcc tcaataacat tggcgtgtat cttcggaggt aattagcttt
     2101 atttcgaacc atttctaaat agccatgatt tttattacct gtaatagctt ttttagcttc
     2161 ttctccatct tgtactaaat cttcccaagg catgattcgt tcaatttcgt caaaaggatt
     2221 actatcatta tcttttgcaa agtgaagagc atcaattaaa gaagcataat gttccaattt
     2281 tttagtagcc aattttcctt tttcttttaa ctgttcttgt gaatcacgtg tccctttgcg
     2341 tttaatactt gctaaaatgc ggtcattaat ctcaatcagt tgatcgatca gatattgatg
     2401 atgattgact aaaaaagcaa taagtaaaga gtatcttttt tcaagctcaa aacgggagaa
     2461 gtcatatgca tcataatttt cccctagtct agctagctga agaaacctgt tccgattaat
     2521 atgggagaca ttaattgtcc cgagtcccat ggaagcaatc acttccactt ttttacaaat
     2581 actcataaag ctttctggat ttgccttacc tggaatgtct tttagccaag cgagtttagt
     2641 tattttcgtc tcttcataaa tttgaaacaa actctctagt ttttcaattt gtatatctgt
     2701 taatgaacag agtaatattg aaaataagtt gttttctgct ttatctcgac agcggcttat
     2761 aatgtcttca agtgtagcta tagatggaaa aataattctt tttcgagtta agaaatcaat
     2821 tgtttttttc attagataga tagagtcatc attttctaaa gctagttcaa ttaaatattc
     2881 tattaactgt ttttgtgtat tagcactacc gaatcgatga tagttgaata cttctaagat
     2941 ctcgttgaag tgatttgcac gtgtatttct atgatcatat gaatttaaat caattgcatc
     3001 aagatggagc tgtcgactca cataagaagt tagtctggtt gatttaatcg gccaattact
     3061 taaagaacac ccaggatacc gggccaaaca aagttgtatc gcaaatccta gtttattgac
     3121 ctttccacgg tgttgattaa taacctccag atcataatca gaaaaactaa aatacgcttt
     3181 aaaatcctct tctgataagt ggtctacaga aagaagttgt tcacgctgtg aagtagttaa
     3241 aattcttttc atagccatat cttaagcata ccttttattg aggtaacggt aaaaggttgt
     3301 tttttaattt agaggcatca agaatttgac gaatagaata ctctttgctg tcatacattt
     3361 ttaaagctaa atcaattgat agcttacctt tacttggacg gccccctttt tttcctcgga
     3421 ctctggctgc cttaagacca gagttagttc gttcaataat aatatcccgt tccagttctg
     3481 ctaaactagc cataactcgg aaaaagaatc ttcccataga cgttgaagta tctacgttat
     3541 cttgaataga tataaaattg acactaagtt cttcaaataa ttcagaaagt tcaatcaaat
     3601 gtttagttga tcgtgaaatt cgatctaact tgtaaatgac aacagaatct ccttcacgta
     3661 gtaggttgat catttcttct aattgcggtc ggtctttttt cgcaccagtt actttttctt
     3721 gaaataattt atcaattcca taatgagtaa gtgcatcaat ttgtaaacta agattttgat
     3781 catccgtact cactcgagca tagccaaaaa tcataaaaaa acctccttaa ttttataatt
     3841 taaatgtacc ataactcatt aagtatacta tataggaact atgaaaaagg aacggatttt
     3901 ggtacttgat ttacttaaaa aacatttaga aaaagcagga aaatgaaaag taccataaac
     3961 ggtcgtttat ggtaccattc aaatttatcc ttattgtaca aaataacagc gaaattttta
     4021 aatctattcc ttatcgatac aaattccccg taggcgctag ggacctcttt agctccttgg
     4081 aagctgtcag tagtatacct aataatttat ctacattccc tttagtaacg tgtaactttc
     4141 caaatttaca aaagcgactc atagaattat ttcctcccgt taaataatag ataactatta
     4201 aaaatagaca atacttgctc ataagtaacg gtacttaaat tgtttacttt ggcgtgtttc
     4261 attgcttgat gaaactgatt tttagtaaac agttgacgat attctcgatt gacccatttt
     4321 gaaacaaagt acgtatatag cttccaatat ttatctggaa catctgtggt atggcgggta
     4381 agttttatta agacactgtt tacttttggt ttaggatgaa agcattccgc tggcagctta
     4441 agcaattgct gaatcgagac ttgagtgtgc aagagcaacc ctagtgttcg gtgaatatcc
     4501 aaggtacgct tgtagaatcc ttcttcaaca atcagataga tgtcagacgc atggctttca
     4561 aaaaccactt ttttaataat ttgtgtgctt aaatggtaag gaatattccc aacaatttta
     4621 tacctctgtt tgttagggaa ttgaaactgt agaatatctt ggtgaattaa agtgacacga
     4681 atgttcagtt ttaatttttc tgacgataag ttgaatagat gactgtctaa ttcaatagac
     4741 gttacctgtt tacttatttt agccagtttc gtcgttaaat gccctttacc tgttccaatt
     4801 tcgtaaacgg tatcggtttc ttttaaattc aattgtttta ttatttggtt gagtaccttt
     4861 tcattcgtta aaaagttttg agaatatttt atatttttgt tcatgtaatc actcctgaag
     4921 tgatacatct ataaataaat acagaagtta aacgatttgt ttgtaatttt agttatctgt
     4981 ttaaaaagtc ataagattag tcactggtag gaattaatct aacgtattta tctgcgtaat
     5041 cactgttttt agtctgtttc aaaacagtag atgttttatc tacattacgc atttggaata
     5101 ccaacatgac gaatccctcc ttcttaatta caaattttta gcatctaatt taacttcaat
     5161 tcctattata caaaatttta agataatgca ctatcaacac actcttaagt ttgcttctaa
     5221 gtcttatttc cataacttta gggttaaaag gatctaggtg aagatccttt ttgataatct
     5281 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5341 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5401 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5461 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5521 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5581 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5641 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5701 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5761 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5821 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5881 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5941 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6001 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6061 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6121 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6181 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     6241 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     6301 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6361 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     6421 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     6481 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     6541 gcggtttttt cctgtttggt catgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6601 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6661 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6721 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     6781 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     6841 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     6901 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     6961 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     7021 gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg
     7081 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     7141 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat
     7201 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
     7261 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
     7321 cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc
     7381 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     7441 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     7501 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
     7561 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     7621 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     7681 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     7741 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     7801 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
     7861 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     7921 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     7981 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     8041 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     8101 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
     8161 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     8221 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     8281 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     8341 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
     8401 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag
     8461 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     8521 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8641 aataaccctg ataaatgctt caataataac ccgcgggggc ccttatttac caataccttt
     8701 atctttaatc tcagcttttt gcccggtgcc atctactaag ccaaaaataa tcttcgcttg
     8761 gtctgtatta tcaatttgtc ctgaaaacgc ttctgcttgt gggccataag catagacatt
     8821 tacatcttct cctgtgtgtc cgccagtagt ccaaccagta ttcgaacgtt catcaaaaat
     8881 ctttcttaat gcctgagcga ttttttcaac atcttttgac tccgccgctg ttttcactgc
     8941 tttcaattca gcctcactta attgaaaatc aatatactca gtcactgttt tttccacatt
     9001 accattttta ataatttctt ctgccatgaa atcaggcgtg cgttttgccg catgtaaagg
     9061 ctccgtcaac cagttgtatt gatctccttt gcctaaagac aagccacctg ttgaatgatc
     9121 agcagttgta accaccaatg tttcaccatc ttttttggca aaatcgatgg ccttctcaaa
     9181 cgccgcttcg aagtcttgca tttcgctcat cgcgccaaca atatcattgc tatgcccggc
     9241 ccaatcaatt tgactacctt caaccattaa aaagaaacct ttttcatttt tatctaaccg
     9301 ttgaagagcc gcttctgtca tatcagctaa tgaagggact tcttccgttc ggtcaatcat
     9361 tttaggtagc ccgcctggtg caaacaagcc taaaattttg tcgtcttggt tctcatttaa
     9421 cgactttttg tctgtgacat gaccataacc cgcttgggaa aattctttga ctaaatcacg
     9481 atctttccgc gcaaataatt cggagccgcc gccaagtaac acatcgactt tgtgttgtcc
     9541 gtcgatttga tcatcaaaat agtcatcggc gatttctgcc atatttttgc gtgaaacatt
     9601 atgtgcgcca tatgcagcag gggttgcatg tgttatttca gatgttgcta caagacccgt
     9661 tgatttcccc acttttttcg cacgttccag cactgtttct gttttggact tgtcattatc
     9721 gagcgcaata gcattattgt aggttttcac tccagcagcc atcgctgtcg ctgcggacgc
     9781 tgaatcggtg acattctctt cttcatcttc tggataagtg gcttgttgcc caactaaata
     9841 ggtatcaaaa gctgtttggg gaacacgctt gtctgagtga ttggctttga aatagcgata
     9901 gcccgttgta tacggattcc ccatgccatc tccaatcata aaaataacat tttttactgg
     9961 tgctttttca gattcagttg ccttcgcttc agcgcatgca acctctgttt gtttttcgcc
    10021 gcttttttgt tcgggatcct tatcgataca aattcctcgt aggcgctcgg gacccctgca
    10081 ggtcgataaa cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa
    10141 cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga
    10201 cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa
    10261 gataatatat cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca
    10321 taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac
    10381 taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt
    10441 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tcaaaacaac tttgaaaaag
    10501 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
    10561 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
    10621 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
    10681 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
    10741 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
    10801 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
    10861 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
    10921 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
    10981 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
    11041 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
    11101 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
    11161 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
    11221 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
    11281 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
    11341 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
    11401 tgaattgttt tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga
    11461 catctaatct tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt
    11521 ctaaaagttc gctagatagg ggtcccgagc gcctacgagg aatttgtatc gatctagagg
    11581 atcattttgt ttattgcaat tgtattgcta ttaatcgcaa catcaaacca aaataaaaac
    11641 ccccttcgac tttcgtcagg gggcttttat ttattcaata atccctcctc tcaataaatc
    11701 tattgttgta cttaattcaa cttccatttc tctgtatctt tcaatacgct cttttaagtc
    11761 cttaatttct ttttttaatt cctcattttc agcaaataac tctttttctt tgtttgtcat
    11821 tttatttccc ccgtttcagc atcaagaacc tttgcataac ttgctctata tccacactga
    11881 taattgccct caaaccataa tctaaaggcg ctagagtttg ttgaaacaat atcttttaca
    11941 tcattcgtat ttaaaattcc aaactccgct cccctaaggc gaataaaagc cattaaatct
    12001 tttgtattta ccaaattata gtcatccact atatctaaaa gtaaattctt caattctctt
    12061 ttttggcttt catcaagtgt tatatagcgg tcaatatcaa aatcattaat gttcaaaata
    12121 tcttttttgt cgtatatatg tttattctta gcaatagcgt cctttgattc atgagtcaaa
    12181 tattcatatg aacctttgat ataatcaagt atctcaacat gagcaactga actattcccc
    12241 aattttcgct taatcttgtt cctaacgctt tctattgtta caggatttcg tgcaatatat
    12301 ataacgtgat agtgtggttt tttatagtgc tttccatttt gtataatatt actattattc
    12361 catgtatctt tatctttttt ttcgtccata tcgtgtaaag gactgacagc catagatacg
    12421 cccaaactct ctaatttttc tttccaatca ttaggaattg agtcaggata taataaaaat
    12481 ccaaaatttc tagctttagt atttttaata gccatgatat aattacctta tcaaaaacaa
    12541 gtagcgaaaa ctcgtatcct tctaaaaacg cgagctttcg cttatttttt ttgttctgat
    12601 tcctttcttg catattcttc tatagctaac gccgcaaccg cagattttga aaaacctttt
    12661 tgtttcgcca tatctgttaa ttttttatct tgctcttttg tcagagaaat cataactctt
    12721 tttttcgatt ctgaaatcac catttaaaaa actccaatca aataatttta taaagttagt
    12781 gtatcacttt gtaatcataa aaacaacaat aaagctactt aaatatagat ttataaaaaa
    12841 cgttggcgaa aacgttggcg attcgttggc gattgaaaaa ccccttaaac ccttgagcca
    12901 gttgggatag agcgtttttg gcacaaaaat tggcactcgg cacttaatgg ggggtcgtag
    12961 tacggaagca aaattcgctt cctttccccc catttttttc caaattccaa atttttttca
    13021 aaaattttcc agcgctaccg ctcggcaaaa ttgcaagcaa tttttaaaat caaacccatg
    13081 agggaatttc attccctcat actcccttga gcctcctcca accgaaatag aagggcgctg
    13141 cgcttattat ttcattcagt catcggcttt cataatctaa cagacaacat cttcgctgca
    13201 aagccacgct acgctcaagg gcttttacgc tacgataacg cctgttttaa cgattatgcc
    13261 gataactaaa cgaaataaac gctaaaacgt ctcagaaacg attttgagac gttttaataa
    13321 aaaatcggat aataaatata tataaacgta tatagatttc ataaagtcta acacactaga
    13381 cttatttact tcgtaattaa gtcgttaaac cgtgtgctct acgaccaaaa ctataaaacc
    13441 tttaagaact ttcttttttt acaagaaaaa agaaattaga taaatctctc atatctttta
    13501 ttcaataatc gcatccgatt gcagtataaa tttaacgatc ccgggtaccg agctcgaatt
    13561 c
//
