LOCUS       40924_30825        6384 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range expression vector pMHE3Tc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Fodor BD, Kovacs AT, Csaki R, Hunyadi-Gulyas E, Klement E, Maroti G,
            Meszaros LS, Medzihradszky KF, Rakhely G, Kovacs KL.
  TITLE     Modular broad-host-range expression vectors for single-protein and 
            protein complex purification
  JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
  PUBMED    14766546
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Fodor BD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of 
            Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
REFERENCE   3  (bases 1 to 6384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2004"; volume: "70"; issue: "2"; pages:
            "712-721"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, 
            Temesvari krt., Szeged, Csongrad 6701, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        198..216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    217..241
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             256..278
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             286..318
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             367..384
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      451..498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      535..963
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1526..2185)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2186..2955)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    3179..4172
                     /note="RP4 mob; oriT"
     regulatory      complement(4262..4377)
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"
     CDS             complement(4686..5873)
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      6258..6373
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
       61 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
      121 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
      181 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
      241 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagcatgact
      301 ggtggacagc aaatgggtcg ggatccgaat tcgagctccg tcgacaagct tgcggccgca
      361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      961 attttaacaa aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     1021 taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     1081 ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc
     1141 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1201 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1261 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1321 tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta gcgaggtgcc
     1381 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
     1441 gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca gcgcacttac
     1501 gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc ggcggctcca
     1561 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc
     1621 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc
     1681 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca
     1741 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat
     1801 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg
     1861 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg
     1921 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc
     1981 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg
     2041 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg
     2101 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc
     2161 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc gcacggttgc
     2221 ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta
     2281 aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc
     2341 cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt
     2401 tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg
     2461 ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg
     2521 cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc
     2581 gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact cgcataggct
     2641 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc
     2701 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt
     2761 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg
     2821 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg
     2881 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag
     2941 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag
     3001 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc gccgtaggcg
     3061 gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg gcccgccgga
     3121 ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag gggcaggcat
     3181 ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg tggcggccag
     3241 tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca ggacgccaga
     3301 gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc gcgagttgct
     3361 gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga cggccagccc
     3421 ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga aggcgcacaa
     3481 gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc accgtgacga
     3541 aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca ggctgtcggc
     3601 caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt ttgcggccgc
     3661 tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc acacgcgcat
     3721 tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca tcagcccgca
     3781 agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg gaatctcaaa
     3841 gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc ggcaggggta
     3901 tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg ccgatcaagc
     3961 ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg ccctggggcc
     4021 gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg gcgaaaagct
     4081 gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc aggaacgcgg
     4141 gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta ccgaacaact
     4201 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     4261 tagctttatg cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct
     4321 agggtcccca attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg
     4381 gatcaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     4441 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     4501 acccgcagtt gcaaaccctc actgatccgc cgagatgcgc cgcgtgcggc tgctggagat
     4561 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     4621 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
     4681 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
     4741 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
     4801 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat
     4861 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     4921 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     4981 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
     5041 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     5101 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     5161 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     5221 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     5281 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag
     5341 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
     5401 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     5461 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     5521 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     5581 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     5641 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     5701 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     5761 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     5821 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     5881 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctc gcgcagatca
     5941 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     6001 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     6061 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     6121 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
     6181 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct
     6241 ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
     6301 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta
     6361 caagcataaa gctcaaggag atgg
//
