LOCUS       40924_30810       10322 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pMHCluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 10322)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10322)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(705..716)
                     /codon_start=1
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
                     /translation="WELQ"
     CDS             5592..7241
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(7285..7406)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7825..8413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8587..9444)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9445..9549)
                     /label=AmpR promoter
     rep_origin      9576..10000
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    10162..10210
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    10224..10315
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ccgctgaagc cgagatcctg actccagact
       61 cttcttcact gccctgagga cccggggatg gggtctgggg aggtggaaat gcaggggagt
      121 gggggcaaat tcaaaggcct ggcctaggag gcttcgtgga gcagcactga aaaccccttt
      181 cacttccccc aaaccagcca aatgcagcag aggcgacacc tctcctccca gccttctccc
      241 tggtccagcc aggatttaaa ggagatttag ggagacccta aagttcacct agagcaccct
      301 gagcccatct actgtggacc tgcacccagc ttccgtcaac cctgccccac cacacatgga
      361 tactgcccag ggctgggcaa agggcagcgg ttcggggtgt gggtgctggc cagtgccctt
      421 tctgagcccg cagaagtgtg aaccactggg caggttgagg caatggctgc ctaagtgcca
      481 gtcccaggcg agtcttgaga gtacacaagg attcacagct gtagcctgca tcccctttta
      541 aatagagcca ccaccctctc cagccaccgc cagaagccag cataccccct gcaccacagg
      601 tgggagaaac acaccctcag cccacctgcc gtcctcctca gctcctcctg gtgccgagcc
      661 cccttggccc agggtgctcc cttcttccca ctcatttctt cccattgtaa ttcccattac
      721 caagcccttt gtcctctggg cttgtgggaa gatggggttc tcctggccca tttatgcaag
      781 ccatcttgag gggaggcaga cagatgaggg gctggggaag gttatgtgtg aacaggactc
      841 tggggagagg gagggaaggg atggctcccg agagggccag gaggtggagg aaagagtaag
      901 tctcctccaa ctaaacaagt tcagcccctt tggagttcct gccacagctg ggttgcttag
      961 gcaggcctgg gactgtgggc cacagaggtc aggggctgaa aggcactggc tgtcggcccc
     1021 cactgggaac ctgggaagca gtggttcagc cctggactgg atctccccca acccaccaac
     1081 atacccactc ggagcccact cctgctctgt acccagctct gagctcggag ctctgaggac
     1141 tgcggcacgc agagccaagg gccctaagcc ggagaaggag aaaccagtac tggacaatag
     1201 caggtgacag cagaggagaa aaaggggggt agggagggga caaggaaaga cctgaggggg
     1261 tggcagtgga caaagagatt gagcaaccac agggacaagg taaccaggag gaaacagaga
     1321 caaggaaaaa agatgcagga aatgaggagg ccccagaaag acctgcagcg ccgccacact
     1381 ttctcctcat agccaccatc cattctcctt ccctaggcca acccagacag agctccctca
     1441 aaccagggat gcttcctttt catttatcca gctcaattga accacacagc cccagaggcc
     1501 aaggtaattc agcttcctct gtgatttcta ggattctttc agcctttttg tttttgtttt
     1561 tgtttttgtg acggagtctc actctgcttg cccaggctgg agtgcagtgg cacaacctgg
     1621 cttgggccag atccctaggg ccattcctct ggctaggaac agacttggac agcaatgaag
     1681 agataactag aaacaaagga gagaaccgct gaaaaggagc tatgcagaga gacccagggc
     1741 acctaagcaa ccagagcaag agaggacgcg caagagagta cagatcctat gagaatggtc
     1801 gggggctgga tgcagacagc agggttgaga gagctctgag agccaagcca gggagacagc
     1861 aggggtgctg agggggatgg gggagagagg aaaagagagg gagccttgag ggagaagctt
     1921 gaagagctgc gggtggtgat cccgagtgaa ggaaagaatc ctgggtcact tgcaaatgca
     1981 aggagagtca ggaaggcgca aggagattgg agaggaggaa gagcctggag caagatgtgg
     2041 aggcccaggg cgagagaaga ggccagctcg gagagggcag agctcatgtt agaccctgag
     2101 ccgggtggag acacgggcca gggagaatcg gaggcaggcc ccactctgag atggagaagc
     2161 aggcagaggg aatgaaggtg agacagagac agatgcaacg gggagccagg cagaagacag
     2221 gcaacctgtg ccagggaggc cagccttgcc tccttggcac cctcacctgt ggccgaagcc
     2281 tccatccctg tccttatatt ccaggactcc cagcagaatg aggagctgtg tgcaaggcag
     2341 cttagggagg acggagagca atatgtgaga gcagggagag aaggagcctg gggaagggag
     2401 aggacggtac agcggcagac gggcagggct ggggctgagt gaacaccttc cttcctcctc
     2461 cttccacagg agcgggggac tacccccacc acctccactc cccgctgccc tgccaccacc
     2521 acctatacca agtgaggcct gctcagagca cagggttccc tgaggacaaa gcctggtctt
     2581 tgtcacctga ccccgaccca gactgacctg gcgttctcag ctccttatca tgtccccaga
     2641 gctatgggaa ccaagcagtg acgcactggc catgggctaa ccccccggct cgaattctgg
     2701 ggcctcgagt aacctagagc tggggacagg tggcctgcat cccctggggt aatgatttgg
     2761 cataagcacc tgcagaagat ggggatgtgg ggtcaactaa ggtccctcca aggacattga
     2821 gaaaaggtcc aaatgctctg gaaagagagg tgtgttcctg agcccaggaa tggggtgggc
     2881 agcgtcctag ggagctaggg gagaggccac atcttaatgg aaagtgctcc tacccagaga
     2941 agctgacccc tgtcgtccta cccacctcca caccctggag ctatattgag aggtgacagt
     3001 aaactggtgg gaggggggct gggagggtgt tcctgggtgt gagggtgggg ggaaggccag
     3061 agcaggggag tctggctttg tttcctgaac acaatgtcca cttagtcata acaggaacag
     3121 cctgctgaag acctgacacc tacagcctct gagagatggc agcattggag gatggggagc
     3181 ggggccttga gggctagggt gcttgaggac acccacaaag aagggaatgt gagtataagc
     3241 cctggagggc ttggaaaggg gcaacaaaat tctgcctggg gagggggcag gtgcagagcc
     3301 ccaccccgat ggaatgcagt gcctggggac cagatggtgt aaatccccag gcctgccagg
     3361 cacctgcacc ctctggcagc ccggtgaggc agaagggaca gcccctcacc cacccccctc
     3421 ctctctcagg ccctaggatt agcaccatct tcttctgccc accccacccc aggagcagag
     3481 ggtagcaggg gagggcaaaa gcctatgtgt gcaaatgcgg atgtgcaaat gtgtgcatcc
     3541 atgtacacaa aagaaagtag aaaagaaatc tgcaagcttg agtgggtaag tgtgaaaaat
     3601 ctgcatgtgt ggctgaagat gggcacagac acggtcaagt ctgtatgtga gagtgctgaa
     3661 ctggggttct gtgtgaaaat ctgcctgagg cggcagggag aatcactgcc attgcgtgag
     3721 caggttggat gttggccact ctatcaggag cattagggaa ggggtgggga ctccagacgt
     3781 gtccccaaac cagggtggcc tcaagacctt gggagaacac ttgtctgaag acttggggaa
     3841 cagaaggaga ccaggcatgg cacttatgca gactgaggcc aggacagaat ttcctgacaa
     3901 aagaaaactg agccatggag atggacaaca gatcccttcc ctgggcacat actgcagctt
     3961 ttagtcccta gcacttgggg ctccagtact aacagcagga agatgctccc agccttggga
     4021 ctgtgtgagg gatgtcagaa tggaaaggag aggctgggga acaggggagg aaagcccatg
     4081 gttgggaggc ggaggacagg catttggcct gcaggagaag gtgaccctca cccatgtttt
     4141 cagtctcact tcgggggaaa aataactgag gtaagggcca tggcagggtg ggagaggcgg
     4201 tgtgagaagg tcctgtcttc ccacatatct gctcatcagc cctttgaagg ggaggtaatg
     4261 tgccctaagg actaaaaaaa ggccgtggag ccagagaggc tggggcagca gacctttctt
     4321 gggcaaatca gggggccctg ctgtcctcct gtcacctcca gagccaaagg atcaaaggag
     4381 gaggagccag gaggggagag aggtgggagg gagggtcctc cggaaggact ccaaatttag
     4441 acagagggtg ggggaaacgg gatataaagg aactggagct ttgaggacag atagagagac
     4501 tctgcggccc aggtaagagg aggtttgggg tgggatgccc tgcagcccgt ccacagagcc
     4561 cccaccgtga gggacctcct tcaccaggag tggggtgcag gtcagttgga ggcctaaggg
     4621 ctctattaaa actgcctatc tccaggccca gggaagttcc ccctgacaca ggaggttcca
     4681 caggaaaccc agaaacctct tttctccttc tctgactctc catttctttc tctgcatcat
     4741 tctgagtctc ctgcatgttg tctccatctt tccatcttca cttcctcctt tggatggctt
     4801 ccttcccttg atcctggctt ttatcttgcc tcttggtctt catcgacact tgtcacaatc
     4861 atgcttcttt gtctctctcc cttgtccttc cttcttggca cttgttctca cctccctgcc
     4921 tctctgcttc taaccctgtt tccacacccc gtccctcgca ctcatattga ctcggtgccc
     4981 tttcttttct gcctctgcgt ctttcccttt ctgactccct ggtctgtcct gcctgtctgc
     5041 gctctggggc tgcctccatc cccgggtggc ctgcctctgt tgttcttcac tctcctcatc
     5101 tgttcttctc tctgcccggc tctacctctg ttgttccttg ctccacccac ggtccagatt
     5161 cttcaggatt ctccgtgaag ggataaccag gtgagaactg cccccatttt ctctgcagag
     5221 actggggcat gcttctcctg ggagccggat tgctggacca ggggtctgct gtcccaagca
     5281 ctcagcgcca acccttagca tactccagcc aatgccaccc cagggaaacc ccttacagag
     5341 attgtccttc agcatcacct cagagggcag gagaagcaga gccctgagta ggggagggtg
     5401 caacagcagg tgcctctccc agggtggagg agaggagcgg gggtagggag ggggctgcag
     5461 aggacaaagc cactcgctga gcctgggctc cctcaggagt aacatagccc tcctgtctct
     5521 gacccagggg aagcaccaag gatctgcgat ctaagtaagc ttggcattcc ggtactgttg
     5581 gtaaagccac catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
     5641 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     5701 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
     5761 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     5821 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     5881 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     5941 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
     6001 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
     6061 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     6121 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
     6181 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
     6241 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     6301 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     6361 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
     6421 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
     6481 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
     6541 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
     6601 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     6661 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     6721 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
     6781 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     6841 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
     6901 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
     6961 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
     7021 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     7081 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     7141 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     7201 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg
     7261 gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     7321 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     7381 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7441 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     7501 tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     7561 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     7621 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7681 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7741 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7801 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7861 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7921 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7981 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     8041 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8101 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8161 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8221 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8281 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8341 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8401 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8821 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8881 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8941 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9001 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9061 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9121 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9181 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9241 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9301 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9361 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9421 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9481 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9541 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     9601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    10021 gtttacaatt tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10081 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg
    10141 ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt
    10201 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
    10261 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga
    10321 ta
//
