LOCUS       .                       4867 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(1466..2197)
                     /gene="ermC"
                     /label="ermC"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             3969..3986
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(4472..4483)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
ORIGIN
        1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
       61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat
      121 agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag
      181 aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg ctattaaaaa
      241 tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta atgattggaa
      301 agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg atatggacga
      361 aaaaaaagat aaagatacat ggaatagtag tgatgttata cgaaatggaa agcactataa
      421 aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag aaagcgttag
      481 gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga tacttgatta
      541 tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg ctaagaataa
      601 acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg accgctatat
      661 aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata tagtggatga
      721 ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg gagcggagtt
      781 tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta gcgcctttag
      841 attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa aggttcttga
      901 tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc tgaaaatgag
      961 gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag agaaatggaa
     1021 gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata aataaaagcc
     1081 ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta atagcaatac
     1141 aattgcaata aacaaaatga tcgacctcgg gacccctatc tagcgaactt ttagaaaaga
     1201 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
     1261 aagctagctt tttagacatc taaatctagg tactaaaaca attcatccag taaaatataa
     1321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     1381 tgttcttccc cgatcgaccc gattcacaaa aaataggcac acgaaaaaca agttaaggga
     1441 tgcagtttat gcatccctta acttacttat taaataattt atagctattg aaaagagata
     1501 agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt aaggaattgt
     1561 taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc ataacgaaat
     1621 aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt aatctgataa
     1681 gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata cttaatatag
     1741 aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt aataatcttt
     1801 tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca aaaacaattt
     1861 tgcgtattat atccgtactt atgttataag gtatattacc atatatttta taggattggt
     1921 ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta tcgtgatcaa
     1981 caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca gttacgaaat
     2041 tacacctctt tactaattca agggtaaaat ggccttttcc tgagccgatt tcaaagatat
     2101 tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt tttgaagtaa
     2161 taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt aatttggtta
     2221 tatgaatttt gcttattaac gattcattat aaccacttat tttttgtttg gttgataatg
     2281 aactgtgctg attacaaaaa tactaaaaat gcccatattt tttcctcctt ataaaattag
     2341 tataattata gcacggtcga tcttctatat aaaagatata ttatcttatc agtattgtca
     2401 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
     2461 aaatatgggt cgatcgaatt cggtcctcgg gatatgataa gattaatagt tttagctatt
     2521 aatctttttt tatttttatt taagaatggc ttaataaagc ggttactttg gatttttgtg
     2581 agcttggact agaaaaaaac ttcacaaaat gctatactag gtaggtaaaa aaatattcgg
     2641 aggaattttg aaatggcaat cgtttcagca gaaaaattcg taattcgagc tcgcccgggg
     2701 atcgatcctc tagaatgaaa aaaaagatta tctcagctat tttaatgtct acagtgatac
     2761 tttctgctgc agccccgttg tcaggtgttt acgccggatc catgcgacgg acggagcttc
     2821 ttcagctgga aatactaatt ctggtggctc gacaaccaca attacgaata ataattctgg
     2881 aaccaatagc agttcaacta cttataccgt caaatctggt gatactcttt ggggaatctc
     2941 acaaagatat ggaattagtg tcgctcaaat tcaaagtgcg aataatctta aaagtaccat
     3001 tatctacatt ggtcaaaaac ttgtacgtcg acatgaaaaa acatgctatt gcagtaatga
     3061 tgatcgccgt attttctgag tcggtttatg cggagtctgc cttatttatt ccggacgtct
     3121 ctcctgatag cgtcacgaca tccctttccg tgggtgtgtt aaatggtaaa tccagggagc
     3181 tggtttatga taccgacacc gggcggaagc tgagtcaact ggattggaaa ataaaaaatg
     3241 tcgccacgtt gcagggggat ttatcatggg aaccctattc gttcatgacg ctggacgccc
     3301 gcggctggac gtctttggcg tcgggatcgg gtcatatggt tgaccatgac tggatgagca
     3361 gtgagcagcc aggctggacc gatcgttcaa ttcatccgga caccagcgtc aactatgcta
     3421 atgaatacga tttgaacgtg aaaggttggt tattgcaggg cgataactac aaggcgggcg
     3481 taacagcggg ctatcaggaa acccgtttta gctggacggc aagaggcggg tcttatattt
     3541 atgataatgg tcgatatatt ggtaattttc ctcatggcgt gcgcggcata ggttatagcc
     3601 agcgtttcga aatgccctat atcgggctgg cgggtgatta tcgtattaat gactttgagt
     3661 gtaatgtact gtttaaatac agcgactggg taaatgcgca tgataatgac gaacactaca
     3721 tgcgcaaact taccttccgt gaaaaaacgg aaaattcacg atattatggc gcttctattg
     3781 acgccggata ttatattacc agtaatgcaa aaatctttgc tgagtttgct tacagtaaat
     3841 atgaagaagg taagggcggt acgcaaatca tagataaaac cagcggtgat acggcgtatt
     3901 ttggtggcga tgccgcaggt atagctaata ataactatac ggttaccgcg gggttgcagt
     3961 atcgcttcca ccaccaccac caccactaga agcttgcaaa gtctgaaaac gaaggtggca
     4021 gctgccgttg aagcggccaa gacagttggt aaaggcgacg gtacaaccgg tactagcgac
     4081 aaaggcggcg gtcaaggtac cccggcgcta cgatatttgg agttgaggtt caaagtcaaa
     4141 tggtactgat gaccggtaaa atttaatatt ttgaaccttg cttaggcagc tgacttcaca
     4201 ttgttgagat cagctgcctt ttgcttatag ttcattgagt agaaacggtt ctgttgcgaa
     4261 gtttgaaaat caaacgcaag ctcgattttt tattaaaacg tctcaaaatc gtttctgaga
     4321 cgttttagcg tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag
     4381 cgtaaaagcc cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagattatga
     4441 aagccgatga ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct
     4501 caagggagta tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat
     4561 tttgccgagc ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg
     4621 ggggaaagga agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa
     4681 tttttgtgcc aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg
     4741 ccaacgaatc gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta
     4801 ttgttgtttt tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt
     4861 ttttaat
//
