LOCUS       40924_30630        4501 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial selection and production vector pMES4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Pellis M, Pardon E, Muyldermans S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2009) Cellular and Molecular Immunology, VUB-VIB, 
            Pleinlaan 2, Brussels, Brussels 1050, Belgium
REFERENCE   2  (bases 1 to 4501)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4501)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2009) Cellular and Molecular Immunology, VUB-VIB, Pleinlaan 
            2, Brussels, Brussels 1050, Belgium"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      211..215
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     225..290
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             340..357
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    361..390
                     /label=HA-tag
                     /note="HA-tag"
     gene            391..1623
                     /gene="M13 geneIII"
                     /label=M13 geneIII
     primer_bind     complement(1630..1646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1859..2314
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2596..2700
                     /label=AmpR promoter
     CDS             2701..3558
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3732..4320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacaagctta aggagacagt acatatgaaa tacctattgc
      241 ctacggcagc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc
      301 tgcaggagtc tagaggggac ccaggtcacc gtctcctcac accaccatca ccatcactag
      361 tacccgtacg acgttccgga ctacggttcc actgttgaaa gttgtttagc aaaacctcat
      421 acagaaaatt catttactaa cgtctggaaa gacgacaaaa ctttagatcg ttacgctaac
      481 tatgagggct gtctgtggaa tgctacaggc gttgtcgttt gtactggtga cgaaactcag
      541 tgttacggta catgggttcc tattgggctt gctatccctg aaaatgaggg tggtggctct
      601 gagggtggcg gttctgaggg tggcggttct gagggtggcg gtactaaacc tcctgagtac
      661 ggtgatacac ctattccggg ctatacttat atcaaccctc tcgacggcac ttatccgcct
      721 ggtactgagc aaaaccccgc taatcctaat ccttctcttg aggagtctca gcctcttaat
      781 actttcatgt ttcagaataa taggttccga aataggcagg gtgcattaac tgtttatacg
      841 ggcactgtta ctcaaggcac tgaccccgtt aaaacttatt accagtacac tcctgtatca
      901 tcaaaagcca tgtatgacgc ttactggaac ggtaaattca gagactgcgc tttccattct
      961 ggctttaatg aggatccatt cgtttgtgaa tatcaaggcc aatcgtctga cctgcctcaa
     1021 cctcctgtca atgctggcgg cggctctggt ggtggttctg gtggcggctc tgagggtggc
     1081 ggctctgagg gtggcggctc tgagggtggc ggttctgagg gtggcggctc tgagggtggc
     1141 ggttccggtg gcggctccgg ttccggtgat tttgattatg aaaaaatggc aaacgctaat
     1201 aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc taaaggcaaa
     1261 cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg tgacgtttcc
     1321 ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc ccaaatggct
     1381 caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata tttaccttct
     1441 ttacctcagt cggttgaatg tcgcccttat gtctttggcg ctggtaaacc atatgaattt
     1501 tctattgatt gtgacaaaat aaacttattc cgtggtgtct ttgcgtttct tttatatgtt
     1561 gccaccttta tgtatgtatt ttcgacgttt gctaacatac tgcgtaataa ggagtcttaa
     1621 taagaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1681 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1741 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     1801 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     1861 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1921 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1981 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2041 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     2101 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2161 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     2221 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2281 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     2341 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2521 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2581 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2641 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2701 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2761 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2821 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2881 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2941 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3001 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3061 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3121 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3181 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3241 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3301 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3361 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3421 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3481 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3601 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3841 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4501 g
//
