LOCUS       .                       5772 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      155..1137
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        1164..1268
                     /label="AmpR promoter"
     CDS             1269..2126
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2300..2888
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3176..3197
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3212..3242
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3250..3266
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3274..3290
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3347..3615
                     /label="SNR52 promoter"
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        3636..3710
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      3715..3734
                     /label="SUP4 terminator"
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      3740..3987
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(4006..4024)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4034..4050)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4728..5387)
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(5388..5574)
                     /label="HIS3 promoter"
ORIGIN
        1 ccgtgccatg ccatgccttc acatatagta caggcaacac gcagatatag gtgcgacgtg
       61 aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa
      121 cgctttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg
      181 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
      241 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
      301 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
      361 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
      421 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
      481 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
      541 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
      601 ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag
      661 taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca
      721 aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat
      781 tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct
      841 ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg
      901 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
      961 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
     1021 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     1081 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttcag
     1141 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1201 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1261 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1321 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1381 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1441 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1501 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1561 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1621 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1681 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1741 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1801 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1861 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1921 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1981 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2041 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2101 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2281 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2341 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2401 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2461 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2521 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2581 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2641 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2701 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2761 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2821 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2881 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2941 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3001 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3061 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     3241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     3301 ccaagcgcgc gattaaccct cactaaaggg aacaaaagct ggagcttctt tgaaaagata
     3361 atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct ttcgagtata
     3421 tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg ccctcttggg
     3481 ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa agattttggt
     3541 caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact tctccgcagt
     3601 gaaagataaa tgatcgatac gttctctatg gaggagtttt agagctagaa atagcaagtt
     3661 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg gtgctttttt
     3721 tgttttttat gtcttcgagt catgtaatta gttatgtcac gcttacgttc acgccctccc
     3781 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     3841 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     3901 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     3961 ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt
     4021 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4081 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4141 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     4201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4321 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cgttgaacat
     4441 tcttggctgg tcgaatcatt tagacacggg catcgtcctc tcgaaaggtg catctgtgcg
     4501 gtatttcaca ccgcatagat ccgtcgagtt caagagaaaa aaaaagaaaa agcaaaaaga
     4561 aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga atgatgcatt accttgtcat
     4621 cttcagtatc atactgttcg tatacatact tactgacatt cataggtata catatataca
     4681 catgtatata tatcgtatgc tgcagcttta aataatcggt gtcactacat aagaacacct
     4741 ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg cttctcttat ggcaaccgca
     4801 agagccttga acgcactctc actacggtga tgatcattct tgcctcgcag acaatcaacg
     4861 tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa aatgcgggat catctcgcaa
     4921 gagagatctc ctactttctc cctttgcaaa ccaagttcga caactgcgta cggcctgttc
     4981 gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag gcgcaaatcc tgatccaaac
     5041 ctttttactc cacgcacggc ccctagggcc tctttaaaag cttgaccgag agcaatcccg
     5101 cagtcttcag tggtgtgatg gtcgtctatg tgtaagtcac caatgcactc aacgattagc
     5161 gaccagccgg aatgcttggc cagagcatgt atcatatggt ccagaaaccc tatacctgtg
     5221 tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct
     5281 gggaagatcg agtgctctat cgctagggga ccacccttta aagagatcgc aatctgaatc
     5341 ttggtttcat ttgtaatacg ctttactagg gctttctgct ctgtcatctt tgccttcgtt
     5401 tatcttgcct gctcattttt tagtatattc ttcgaagaaa tcacattact ttatataatg
     5461 tataattcat tatgtgataa tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg
     5521 gaaaaaaaaa atgaaaatca ttaccgaggc ataaaaaaat atagagtgta ctagaggagg
     5581 ccaagagtaa tagaaaaaga aaattgcggg aaaggactgt gttatgactt ccctgactaa
     5641 tgccgtgttc aaacgatacc tggcagtgac tcctagcgct caccaagctc ttaaaacggg
     5701 aatttatggt gcactctcag tacaatctgc tctgatgccg cagtgcctat tgatgatctg
     5761 gcggaatgtc tg
//
