LOCUS       62056_16710       10566 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..1240)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1346..1993
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     promoter        complement(3964..4041)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        4275..4303
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             4415..5131
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     rep_origin      complement(5741..6286)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      complement(6996..7190)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7259..8329)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8761..9387)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tagttctagc tagttctaga gcggcctatc gtttccacga tcagcgatcg gctcgttgcc
       61 ctgcgccgct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag agggcagcgc
      121 ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc gccgtgaaga
      181 ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct tgcagctgcc
      241 cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc attccgatgc
      301 cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc gcgaaggcaa
      361 gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc ctttcgccga
      421 gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga atgccaaatg
      481 cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg ccgaaaatga
      541 cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc gccatcaggg
      601 cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg agagcctccc
      661 ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag cccgtcagga
      721 aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag aaaccgccca
      781 tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg ctcatgaagc
      841 cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg ccggctaccg
      901 ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc caaaggaaag
      961 gcgctgtcgc catgatggcg tagtcgacag tggcgccggc cagcgagacg agcaagattg
     1021 gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca
     1081 acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat
     1141 cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga
     1201 cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc
     1261 tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat
     1321 gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc
     1381 cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga
     1441 acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct
     1501 cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga
     1561 cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc
     1621 ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac
     1681 ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa
     1741 tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga
     1801 cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg
     1861 ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc
     1921 ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag
     1981 aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc
     2041 atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg
     2101 ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg
     2161 gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt ccgcgcgccg
     2221 gcctcgtgcg cctggcgctg aggtctgcct cgtgaagaag gtgttgctga ctcataccag
     2281 gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat gagagctttg
     2341 ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg
     2401 tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc
     2461 cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
     2521 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2581 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2641 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2701 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2761 ctgaatccgg tgagaatggc aaaagccgcc acggcgatat cggatccgaa cgccagcaag
     2821 acgtagccca gcgcgtcggc cagcttgcaa ttcgcgctaa cttacattaa ttgcgttgcg
     2881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2941 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3001 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3061 ccacgtggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     3121 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     3181 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     3241 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     3301 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     3361 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     3421 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     3481 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     3541 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     3601 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     3661 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     3721 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     3781 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     3841 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     3901 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     3961 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     4021 tttgcaccat tcgatggtgt caacgtaaat gcatgccgct tcgccttcgc gcgcgaattg
     4081 caagctgatc cgggcttatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca
     4141 tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag
     4201 gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat
     4261 tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg
     4321 ataacaattt cacacaggaa acagaattcg agctcccggg taccggaagc ttcgtactcg
     4381 aggttcacta gtgtctgcag cgagagatct gcccatggtg agcaagggcg aggagctgtt
     4441 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4501 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4561 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4621 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4681 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4741 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4801 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4861 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4921 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4981 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5041 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5101 gatcactctc ggcatggacg agctgtacaa gtagtcgagg cctcggacta gcggatcagt
     5161 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     5221 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     5281 cgcgagcagg ggaattgatc cggtggatga ccttttgaat gacctttaat agattatatt
     5341 actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt ttcacaaaac
     5401 ggtttacaag cataaagctc ctcggactag cctggtatga gtcagcaaca ccttcttcac
     5461 gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     5521 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     5581 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     5641 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     5701 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     5761 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     5821 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     5881 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     5941 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     6001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     6061 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     6121 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     6181 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     6241 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     6301 taaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgccctgg tatgagtcag
     6781 caacaccttc ttcacgaggc agacctcagc gccggcggtc gagtggcgac ggcgcggctt
     6841 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     6901 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     6961 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     7021 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     7081 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     7141 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     7201 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     7261 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     7321 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     7381 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     7441 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     7501 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     7561 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     7621 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     7681 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     7741 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     7801 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     7861 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     7921 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     7981 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     8041 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     8101 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
     8161 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     8221 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     8281 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
     8341 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
     8401 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
     8461 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
     8521 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
     8581 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
     8641 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
     8701 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
     8761 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
     8821 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
     8881 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
     8941 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
     9001 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
     9061 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
     9121 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
     9181 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
     9241 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
     9301 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
     9361 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
     9421 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
     9481 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
     9541 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
     9601 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
     9661 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
     9721 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
     9781 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
     9841 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
     9901 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
     9961 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    10021 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    10081 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    10141 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    10201 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    10261 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    10321 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    10381 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    10441 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    10501 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    10561 ggatca
//
