LOCUS       .                       8042 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    189..210
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        225..255
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    263..279
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     287..303
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        875..977
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             978..1634
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     primer_bind     complement(2621..2637)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3585..4397)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        4748..4852
                     /label="AmpR promoter"
     CDS             complement(6339..7286)
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     rep_origin      complement(7334..7556)
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
       61 tgagcaccgc cgccgcaagg aatggtgcca atacgcaaac cgcctctccc cgcgcgttgg
      121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      301 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc catgaccctg
      361 aatatgatgc tcgataacgc cgtacccgag gcgattgccg gtgcgctgac tcaacaacat
      421 ccggggctat tttttacgat ggtcgaacag gcatcggtag ccatttccct caccgatgcc
      481 cgggcgaata ttatctacgc caacccggcg ttttgccgcc agagcggata ttcgctggcg
      541 caactgctca atcaaaaccc gcgcctgctg gccagcagcc agacgccgcg agaaatctac
      601 cagcagatgt ggcaaaccct gcttcagcgc cagccgtggc gcggccagct gattaatcag
      661 cgccgcgacg gcggcctgta tctggtggat atcgacatca cgccggtgct gaatccccag
      721 ggcgagctgg agcattatct ggcgatgcag cgggatatca gcgtcagcta taccctggaa
      781 cagcggctgc gcaatcatat gacgctgatg gaggcggtgc tcaacaacat ccccgccgcc
      841 gtggtcgtgg tggatgagca ggatcgggtg gtgatgatcg gcacgtaaga ggttccaact
      901 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      961 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1021 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1081 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1141 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     1201 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1261 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1321 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1381 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     1441 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1501 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1561 tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1621 ggcagggcgg ggcgtaatgg ataatctcgc ctacaaaacg tactgcgcgg actgcggcgg
     1681 aaaagagctg ctggtcgagc tccagatttc accgcgcaaa atggggcccg gcgcggagca
     1741 aatcctgccg gtggtggttc gcggcgcggt ccgctggctg tcggtgacct gctgggcgct
     1801 gcccggcgta agcgaagaag ccagccgcta ttttgtcgac agcgccccgg cgcgcacgct
     1861 ggtggtgatc gccgactgta cccagcagcg ccagcagcag gagcagggcc gtctcgaccg
     1921 tctgaagcag cagatgaccg ccggaaagct gctggccgcc attcgcgagt cgctggacgc
     1981 ggcgctgatt cagcttaatt gcccgatcaa tatgctggcg gcggcccgtc ggctgaacgg
     2041 cgaaggcagc ggcaacgtgg cgctggacgc ggcatggcgc gaaggcgaag aagccatggc
     2101 gcgactgcag cgctgccgcc cttctctcga gctggaaagc agcgccgtct ggccgcttca
     2161 gcccttcttt gacgacctgt acgccctcta ccgcacccgc tttgacgacc gcgcgcggct
     2221 gcgggttgac atggcatcgc cgcatctggt cggcttcggc cagcgtaccc agatgctggc
     2281 ctgcttaagt ttatggctcg atcggacgct ggccctcgcc gctgaactgc cctccgtacc
     2341 gctggagatc gagctttatg ccgaagagga cgagggctgg ctctcgctgt atctcaacga
     2401 caatgtaccg ctgctgcagg tgcgctacgc ccactcaccc gatgctttaa actctcccgg
     2461 caaagggatg gagctgcggc tgatccaaac gctggtcgcc taccatcgcg gcgcgattga
     2521 actggcttcg cgcccgcagg gaggcaccag cctcgttctg cgattcccgc tctttgatac
     2581 cctgaccgga gatgagcaat gaggtaccga gctcgaattc actggccgtc gttttacaac
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2761 gcctgaatgg cgaatgagct tatcgatgat aagctgtcaa acatggcctg tcgcttgcgg
     2821 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     2881 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     2941 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     3001 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     3061 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga
     3121 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
     3181 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
     3241 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccgttt ttatcaggct
     3301 ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc
     3361 aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     3421 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
     3481 gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag
     3541 caaccaggcg tttaagggca ccaataactg ccttaaaaaa attagaaaaa ctcatcgagc
     3601 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     3661 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     3721 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     3781 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     3841 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     3901 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     3961 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     4021 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     4081 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     4141 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     4201 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     4261 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     4321 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc
     4381 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     4441 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     4501 tggctttccc tgcagggttt gcagtcagag tagaatagaa gtatcacgtc tcattttcgc
     4561 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     4621 aagtgatctt ccgtcacagg tatttattcg aagacgaaag ggcctcgtga tacgcctatt
     4681 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5101 tccccacagc aagatgcaac tttcgccaga tacgacggcg ctctttgccg tgctttctga
     5161 ctttccattc gccttcacca aagaccttca gcccggtgga atcaatcacc aggtgtgcga
     5221 tttcaccccg ggtggacgtt ttgaaactga cattaaccga ctttgcccgc ttactgacac
     5281 tggtgtaatc cgggcagcgc aacggaacgt tcatcagggc aaaaatggaa tcaataaaac
     5341 cctgcgcagc ccgcagggtc agccggaata cgcgtttaat caccagaacg gtggtgatgg
     5401 cgagatcaga atagcgctgg ggccttcctc gtgatgaagg tgttgccgac tcataccagg
     5461 cctgaatcgc ctcatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt
     5521 tgtaggtgga ccagttggtg attctgaact tttgctttgc cacggaatgg tctgtgttgt
     5581 cgggaggatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc
     5641 actttcagta tggatttggg tgttacgggg gggggttggt gcttaaaatc gcgctgagga
     5701 tttctgatgg tgttaagcgg gcggttttga gatgtaaact cgcccgttta acataatgga
     5761 tcttgcgcgc accgcccgaa caccactcgc cacaaaaaac cgccggaacg tccaaaagta
     5821 cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca
     5881 gatccggctt cagccggttt gccggctgaa agcgctattt cttccagaat tgccatgatt
     5941 ttttccccac gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca
     6001 gcatcgcctg tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt
     6061 gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta
     6121 catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa
     6181 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     6241 acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg
     6301 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     6361 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     6421 tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     6481 gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg
     6541 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     6601 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     6661 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     6721 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     6781 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     6841 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     6901 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     6961 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     7021 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     7081 acaccataag cattttccct actgatgttc atcatctgaa cgtattggtt ataagtgaac
     7141 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     7201 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     7261 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     7321 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     7381 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     7441 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     7501 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     7561 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     7621 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     7681 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     7741 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     7801 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     7861 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     7921 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     7981 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcgggga
     8041 tc
//
